Novel_circ_003686 regulates the osteogenic differentiation of MSCs in patients with myeloma bone disease through miR-142-5p/IGF1 axis
Objectives: Circ_003686 is a novel_circRNA with abnormally low expression found in the samples of multiple myeloma bone disease (MBD) patients. The current research intended to investigate the effects of novel_circ_003686 in osteogenesis-induced differentiation of bone marrow mesenchymal stem cells...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
|
| Series: | Journal of Bone Oncology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212137423000428 |
| _version_ | 1797390476527009792 |
|---|---|
| author | Ling Qiu Lei Ma Dan Chen Nan Zhang Jiao Cai Qian Zhang Xiao Wang Hai Yi Hao Yao Fang-Yi Fan |
| author_facet | Ling Qiu Lei Ma Dan Chen Nan Zhang Jiao Cai Qian Zhang Xiao Wang Hai Yi Hao Yao Fang-Yi Fan |
| author_sort | Ling Qiu |
| collection | DOAJ |
| description | Objectives: Circ_003686 is a novel_circRNA with abnormally low expression found in the samples of multiple myeloma bone disease (MBD) patients. The current research intended to investigate the effects of novel_circ_003686 in osteogenesis-induced differentiation of bone marrow mesenchymal stem cells (BMSCs) in MBD. Methods: BMSCs were extracted from MBD patients and normal participants, the pcDNA3.1 encoding the circ_003686 (ov-circ_003686), miR-142-5p-mimic/inhibitor and siRNA oligonucleotides targeting insulin like growth factor 1 (IGF1, si-IGF1) were applied to intervene circ_003686, miR-142-5p and IGF1 levels, respectively. Results: Results showed that ov-circ_003686 could mediate the osteogenesis-induced differentiation of MBD-BMSC, and luciferase assay and RIP experiments confirmed that circ_003686 could bind to miR-142-5p. MiR-142-5p-inhibitor helped osteogenesis-induced differentiation, while miR-142-5p-mimic inhibited osteogenesis-induced differentiation and reversed the promoting effect of ov-circ_003686, suggesting that circ_003686/miR-142-5p axis participated in osteogenesis-induced differentiation of MBD-BMSC. In addition, miR-142-5p binds to the target gene IGF1 and negatively adjust its expression. Si-IGF1 significantly inhibited the osteogenesis-induced differentiation and reversed the promotion effects of miR-142-5p-inhibitor and ov-circ_003686. Moreover, circ_003686/miR-142-5p/IGF1 axis meaningfully regulates protein expressions in the PI3K/AKT pathway. Conclusion: In conclusion, this research confirmed that circ_003686 regulated the osteogenesis-induced differentiation of MBD-BMSC by sponging miR-142-5p and mediating IGF1, and the PI3K/AKT pathway may also be involved. |
| first_indexed | 2024-03-08T23:12:24Z |
| format | Article |
| id | doaj.art-4d53983fd950438c8b032bfa7256d576 |
| institution | Directory Open Access Journal |
| issn | 2212-1374 |
| language | English |
| last_indexed | 2024-03-08T23:12:24Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Bone Oncology |
| spelling | doaj.art-4d53983fd950438c8b032bfa7256d5762023-12-15T07:23:58ZengElsevierJournal of Bone Oncology2212-13742023-12-0143100509Novel_circ_003686 regulates the osteogenic differentiation of MSCs in patients with myeloma bone disease through miR-142-5p/IGF1 axisLing Qiu0Lei Ma1Dan Chen2Nan Zhang3Jiao Cai4Qian Zhang5Xiao Wang6Hai Yi7Hao Yao8Fang-Yi Fan9Department of Hematology and Hematopoietic Stem Cell Transplantation Center, General Hospital of the Chinese People’s Liberation Army Western Theatre, Chengdu, SiChuan, ChinaDepartment of Hematology and Hematopoietic Stem Cell Transplantation Center, General Hospital of the Chinese People’s Liberation Army Western Theatre, Chengdu, SiChuan, ChinaDepartment of Hematology and Hematopoietic Stem Cell Transplantation Center, General Hospital of the Chinese People’s Liberation Army Western Theatre, Chengdu, SiChuan, ChinaDepartment of Hematology and Hematopoietic Stem Cell Transplantation Center, General Hospital of the Chinese People’s Liberation Army Western Theatre, Chengdu, SiChuan, ChinaDepartment of Hematology and Hematopoietic Stem Cell Transplantation Center, General Hospital of the Chinese People’s Liberation Army Western Theatre, Chengdu, SiChuan, ChinaDepartment of Hematology and Hematopoietic Stem Cell Transplantation Center, General Hospital of the Chinese People’s Liberation Army Western Theatre, Chengdu, SiChuan, ChinaDepartment of Hematology and Hematopoietic Stem Cell Transplantation Center, General Hospital of the Chinese People’s Liberation Army Western Theatre, Chengdu, SiChuan, ChinaDepartment of Hematology and Hematopoietic Stem Cell Transplantation Center, General Hospital of the Chinese People’s Liberation Army Western Theatre, Chengdu, SiChuan, ChinaCorresponding authors.; Department of Hematology and Hematopoietic Stem Cell Transplantation Center, General Hospital of the Chinese People’s Liberation Army Western Theatre, Chengdu, SiChuan, ChinaCorresponding authors.; Department of Hematology and Hematopoietic Stem Cell Transplantation Center, General Hospital of the Chinese People’s Liberation Army Western Theatre, Chengdu, SiChuan, ChinaObjectives: Circ_003686 is a novel_circRNA with abnormally low expression found in the samples of multiple myeloma bone disease (MBD) patients. The current research intended to investigate the effects of novel_circ_003686 in osteogenesis-induced differentiation of bone marrow mesenchymal stem cells (BMSCs) in MBD. Methods: BMSCs were extracted from MBD patients and normal participants, the pcDNA3.1 encoding the circ_003686 (ov-circ_003686), miR-142-5p-mimic/inhibitor and siRNA oligonucleotides targeting insulin like growth factor 1 (IGF1, si-IGF1) were applied to intervene circ_003686, miR-142-5p and IGF1 levels, respectively. Results: Results showed that ov-circ_003686 could mediate the osteogenesis-induced differentiation of MBD-BMSC, and luciferase assay and RIP experiments confirmed that circ_003686 could bind to miR-142-5p. MiR-142-5p-inhibitor helped osteogenesis-induced differentiation, while miR-142-5p-mimic inhibited osteogenesis-induced differentiation and reversed the promoting effect of ov-circ_003686, suggesting that circ_003686/miR-142-5p axis participated in osteogenesis-induced differentiation of MBD-BMSC. In addition, miR-142-5p binds to the target gene IGF1 and negatively adjust its expression. Si-IGF1 significantly inhibited the osteogenesis-induced differentiation and reversed the promotion effects of miR-142-5p-inhibitor and ov-circ_003686. Moreover, circ_003686/miR-142-5p/IGF1 axis meaningfully regulates protein expressions in the PI3K/AKT pathway. Conclusion: In conclusion, this research confirmed that circ_003686 regulated the osteogenesis-induced differentiation of MBD-BMSC by sponging miR-142-5p and mediating IGF1, and the PI3K/AKT pathway may also be involved.http://www.sciencedirect.com/science/article/pii/S2212137423000428Novel_circ_003686miR-142-5pIGF1Multiple myeloma bone diseaseBone marrow mesenchymal stem cellsOsteogenesis-induced differentiation |
| spellingShingle | Ling Qiu Lei Ma Dan Chen Nan Zhang Jiao Cai Qian Zhang Xiao Wang Hai Yi Hao Yao Fang-Yi Fan Novel_circ_003686 regulates the osteogenic differentiation of MSCs in patients with myeloma bone disease through miR-142-5p/IGF1 axis Journal of Bone Oncology Novel_circ_003686 miR-142-5p IGF1 Multiple myeloma bone disease Bone marrow mesenchymal stem cells Osteogenesis-induced differentiation |
| title | Novel_circ_003686 regulates the osteogenic differentiation of MSCs in patients with myeloma bone disease through miR-142-5p/IGF1 axis |
| title_full | Novel_circ_003686 regulates the osteogenic differentiation of MSCs in patients with myeloma bone disease through miR-142-5p/IGF1 axis |
| title_fullStr | Novel_circ_003686 regulates the osteogenic differentiation of MSCs in patients with myeloma bone disease through miR-142-5p/IGF1 axis |
| title_full_unstemmed | Novel_circ_003686 regulates the osteogenic differentiation of MSCs in patients with myeloma bone disease through miR-142-5p/IGF1 axis |
| title_short | Novel_circ_003686 regulates the osteogenic differentiation of MSCs in patients with myeloma bone disease through miR-142-5p/IGF1 axis |
| title_sort | novel circ 003686 regulates the osteogenic differentiation of mscs in patients with myeloma bone disease through mir 142 5p igf1 axis |
| topic | Novel_circ_003686 miR-142-5p IGF1 Multiple myeloma bone disease Bone marrow mesenchymal stem cells Osteogenesis-induced differentiation |
| url | http://www.sciencedirect.com/science/article/pii/S2212137423000428 |
| work_keys_str_mv | AT lingqiu novelcirc003686regulatestheosteogenicdifferentiationofmscsinpatientswithmyelomabonediseasethroughmir1425pigf1axis AT leima novelcirc003686regulatestheosteogenicdifferentiationofmscsinpatientswithmyelomabonediseasethroughmir1425pigf1axis AT danchen novelcirc003686regulatestheosteogenicdifferentiationofmscsinpatientswithmyelomabonediseasethroughmir1425pigf1axis AT nanzhang novelcirc003686regulatestheosteogenicdifferentiationofmscsinpatientswithmyelomabonediseasethroughmir1425pigf1axis AT jiaocai novelcirc003686regulatestheosteogenicdifferentiationofmscsinpatientswithmyelomabonediseasethroughmir1425pigf1axis AT qianzhang novelcirc003686regulatestheosteogenicdifferentiationofmscsinpatientswithmyelomabonediseasethroughmir1425pigf1axis AT xiaowang novelcirc003686regulatestheosteogenicdifferentiationofmscsinpatientswithmyelomabonediseasethroughmir1425pigf1axis AT haiyi novelcirc003686regulatestheosteogenicdifferentiationofmscsinpatientswithmyelomabonediseasethroughmir1425pigf1axis AT haoyao novelcirc003686regulatestheosteogenicdifferentiationofmscsinpatientswithmyelomabonediseasethroughmir1425pigf1axis AT fangyifan novelcirc003686regulatestheosteogenicdifferentiationofmscsinpatientswithmyelomabonediseasethroughmir1425pigf1axis |