Evaluation of chemokines in gingival crevicular fluid in children with dental caries and stainless steel crowns: A clinico-biochemical study
Aims and Objectives: The study was conducted to detect the presence of macrophage inflammatory protein-1α (MIP-1α) and MIP-1β and estimate their levels in gingival crevicular fluid (GCF) in children with dental caries and stainless steel crowns. Materials and Methods: A total of 80 children with pri...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2016-01-01
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| Series: | Journal of Indian Society of Pedodontics and Preventive Dentistry |
| Subjects: | |
| Online Access: | http://www.jisppd.com/article.asp?issn=0970-4388;year=2016;volume=34;issue=3;spage=273;epage=279;aulast=Kumar |
| _version_ | 1818694183214383104 |
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| author | Naveen Kommineni Kumar Veera Kishore Kasa Reddy Prathyusha Padakandla Harshini Togaru Swathi Kalagatla Sarath N Chandra |
| author_facet | Naveen Kommineni Kumar Veera Kishore Kasa Reddy Prathyusha Padakandla Harshini Togaru Swathi Kalagatla Sarath N Chandra |
| author_sort | Naveen Kommineni Kumar |
| collection | DOAJ |
| description | Aims and Objectives: The study was conducted to detect the presence of macrophage inflammatory protein-1α (MIP-1α) and MIP-1β and estimate their levels in gingival crevicular fluid (GCF) in children with dental caries and stainless steel crowns. Materials and Methods: A total of 80 children with primary dentition were selected and categorized into four groups with twenty in each group; Group 1 - healthy subjects, Group 2 - dental caries, Group 3 - dental caries involving the pulp, and Group 4 - stainless steel crowns. GCF samples were collected by an extra-crevicular method with microcapillary pipettes. The GCF samples were quantified by ELISA and the levels of MIP-1α and MIP-1β were determined. Results: MIP-1α and MIP-1β were detected in all the samples. Highest mean concentration in GCF was obtained for Group 3 followed by Groups 2 and 4 while the lowest concentration was seen in Group 1. This suggests that MIP-1α and MIP-1β levels in GCF increased proportionately with the inflammation. Conclusions: GCF serves as a noninvasive diagnostic fluid to measure biomarkers released during dental caries initiation and progression. MIP-1α and MIP-1β chemokines can be considered as novel biomarkers, in biological mechanism underlying the pathogenesis and inflammation in children with dental caries and stainless steel crowns. |
| first_indexed | 2024-12-17T13:25:31Z |
| format | Article |
| id | doaj.art-4d53e1e0dedf4edca7b12dd7ed0870fa |
| institution | Directory Open Access Journal |
| issn | 0970-4388 1998-3905 |
| language | English |
| last_indexed | 2024-12-17T13:25:31Z |
| publishDate | 2016-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Indian Society of Pedodontics and Preventive Dentistry |
| spelling | doaj.art-4d53e1e0dedf4edca7b12dd7ed0870fa2022-12-21T21:46:44ZengWolters Kluwer Medknow PublicationsJournal of Indian Society of Pedodontics and Preventive Dentistry0970-43881998-39052016-01-0134327327910.4103/0970-4388.186754Evaluation of chemokines in gingival crevicular fluid in children with dental caries and stainless steel crowns: A clinico-biochemical studyNaveen Kommineni KumarVeera Kishore Kasa ReddyPrathyusha PadakandlaHarshini TogaruSwathi KalagatlaSarath N ChandraAims and Objectives: The study was conducted to detect the presence of macrophage inflammatory protein-1α (MIP-1α) and MIP-1β and estimate their levels in gingival crevicular fluid (GCF) in children with dental caries and stainless steel crowns. Materials and Methods: A total of 80 children with primary dentition were selected and categorized into four groups with twenty in each group; Group 1 - healthy subjects, Group 2 - dental caries, Group 3 - dental caries involving the pulp, and Group 4 - stainless steel crowns. GCF samples were collected by an extra-crevicular method with microcapillary pipettes. The GCF samples were quantified by ELISA and the levels of MIP-1α and MIP-1β were determined. Results: MIP-1α and MIP-1β were detected in all the samples. Highest mean concentration in GCF was obtained for Group 3 followed by Groups 2 and 4 while the lowest concentration was seen in Group 1. This suggests that MIP-1α and MIP-1β levels in GCF increased proportionately with the inflammation. Conclusions: GCF serves as a noninvasive diagnostic fluid to measure biomarkers released during dental caries initiation and progression. MIP-1α and MIP-1β chemokines can be considered as novel biomarkers, in biological mechanism underlying the pathogenesis and inflammation in children with dental caries and stainless steel crowns.http://www.jisppd.com/article.asp?issn=0970-4388;year=2016;volume=34;issue=3;spage=273;epage=279;aulast=KumarChemokinesdental cariesgingival crevicular fluidmacrophage inflammatory protein-1αmacrophage inflammatory protein-1βpulpal inflammationstainless steel crowns |
| spellingShingle | Naveen Kommineni Kumar Veera Kishore Kasa Reddy Prathyusha Padakandla Harshini Togaru Swathi Kalagatla Sarath N Chandra Evaluation of chemokines in gingival crevicular fluid in children with dental caries and stainless steel crowns: A clinico-biochemical study Journal of Indian Society of Pedodontics and Preventive Dentistry Chemokines dental caries gingival crevicular fluid macrophage inflammatory protein-1α macrophage inflammatory protein-1β pulpal inflammation stainless steel crowns |
| title | Evaluation of chemokines in gingival crevicular fluid in children with dental caries and stainless steel crowns: A clinico-biochemical study |
| title_full | Evaluation of chemokines in gingival crevicular fluid in children with dental caries and stainless steel crowns: A clinico-biochemical study |
| title_fullStr | Evaluation of chemokines in gingival crevicular fluid in children with dental caries and stainless steel crowns: A clinico-biochemical study |
| title_full_unstemmed | Evaluation of chemokines in gingival crevicular fluid in children with dental caries and stainless steel crowns: A clinico-biochemical study |
| title_short | Evaluation of chemokines in gingival crevicular fluid in children with dental caries and stainless steel crowns: A clinico-biochemical study |
| title_sort | evaluation of chemokines in gingival crevicular fluid in children with dental caries and stainless steel crowns a clinico biochemical study |
| topic | Chemokines dental caries gingival crevicular fluid macrophage inflammatory protein-1α macrophage inflammatory protein-1β pulpal inflammation stainless steel crowns |
| url | http://www.jisppd.com/article.asp?issn=0970-4388;year=2016;volume=34;issue=3;spage=273;epage=279;aulast=Kumar |
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