Prevalence of Virulence Genes and Their Association with Antimicrobial Resistance Among Pathogenic E. coli Isolated from Egyptian Patients with Different Clinical Infections
Rehab Mahmoud Abd El-Baky,1,2 Reham Ali Ibrahim,1 Doaa Safwat Mohamed,2 Eman Farouk Ahmed,2 Zeinab Shawky Hashem1 1Department of Microbiology and Immunology, Faculty of Pharmacy, Minia University, Minia 61519, Egypt; 2Department of Microbiology and Immunology, Faculty of Pharmacy, Deraya University,...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2020-04-01
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| Series: | Infection and Drug Resistance |
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| Online Access: | https://www.dovepress.com/prevalence-of-virulence-genes-and-their-association-with-antimicrobial-peer-reviewed-article-IDR |
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| author | Abd El-Baky RM Ibrahim RA Mohamed DS Ahmed EF Hashem ZS |
| author_facet | Abd El-Baky RM Ibrahim RA Mohamed DS Ahmed EF Hashem ZS |
| author_sort | Abd El-Baky RM |
| collection | DOAJ |
| description | Rehab Mahmoud Abd El-Baky,1,2 Reham Ali Ibrahim,1 Doaa Safwat Mohamed,2 Eman Farouk Ahmed,2 Zeinab Shawky Hashem1 1Department of Microbiology and Immunology, Faculty of Pharmacy, Minia University, Minia 61519, Egypt; 2Department of Microbiology and Immunology, Faculty of Pharmacy, Deraya University, Minia 11566, EgyptCorrespondence: Rehab Mahmoud Abd El-BakyDepartment of Microbiology and Immunology, Faculty of Pharmacy, Minia University Tel +20 1092487412Email rehab.mahmoud@mu.edu.egIntroduction: Escherichia (E.) coli can cause intestinal and extra-intestinal infections which ranged from mild to life-threatening infections. The severity of infection is a product of many factors including virulence properties and antimicrobial resistance. Objectives: To determine the antibiotic resistance pattern, the distribution of virulence factors and their association with one another and with some selected resistance genes.Methods: Virulence properties were analyzed phenotypically while antimicrobial susceptibility was tested by Kirby-Bauer agar disc diffusion method. In addition, 64 E. coli isolates were tested for 6 colicin genes, fimH, hlyA, traT, csgA, crl virulence genes and bla−CTX-M-15, bla−oxa-2, and bla−oxa-10 resistance genes by polymerase chain reaction (PCR).Results: Extra-intestinal pathogenic E. coli isolated from urine and blood samples represented a battery of virulence factors and resistance genes with a great ability to produce biofilm. Also, a significant association (P< 0.05) among most of the tested colicin, virulence and resistance genes was observed. The observed associations indicate the importance and contribution of the tested factors in the establishment and the progress of infection especially with Extra-intestinal E. coli (ExPEC) which is considered a great challenging health problem.Conclusion: There is a need for studying how to control these factors to decrease the rate and the severity of infections. The relationship between virulence factors and resistance genes is complex and needs more studies that should be specific for each area.Keywords: E. coli, virulence, resistance, colicin genes, ESBL, bla−CTX-M-15, bla−oxa-2, bla−oxa-10 |
| first_indexed | 2024-12-21T20:28:22Z |
| format | Article |
| id | doaj.art-4d5778fcccd14aaabf7afceb85c0fe6a |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-12-21T20:28:22Z |
| publishDate | 2020-04-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-4d5778fcccd14aaabf7afceb85c0fe6a2022-12-21T18:51:18ZengDove Medical PressInfection and Drug Resistance1178-69732020-04-01Volume 131221123653393Prevalence of Virulence Genes and Their Association with Antimicrobial Resistance Among Pathogenic E. coli Isolated from Egyptian Patients with Different Clinical InfectionsAbd El-Baky RMIbrahim RAMohamed DSAhmed EFHashem ZSRehab Mahmoud Abd El-Baky,1,2 Reham Ali Ibrahim,1 Doaa Safwat Mohamed,2 Eman Farouk Ahmed,2 Zeinab Shawky Hashem1 1Department of Microbiology and Immunology, Faculty of Pharmacy, Minia University, Minia 61519, Egypt; 2Department of Microbiology and Immunology, Faculty of Pharmacy, Deraya University, Minia 11566, EgyptCorrespondence: Rehab Mahmoud Abd El-BakyDepartment of Microbiology and Immunology, Faculty of Pharmacy, Minia University Tel +20 1092487412Email rehab.mahmoud@mu.edu.egIntroduction: Escherichia (E.) coli can cause intestinal and extra-intestinal infections which ranged from mild to life-threatening infections. The severity of infection is a product of many factors including virulence properties and antimicrobial resistance. Objectives: To determine the antibiotic resistance pattern, the distribution of virulence factors and their association with one another and with some selected resistance genes.Methods: Virulence properties were analyzed phenotypically while antimicrobial susceptibility was tested by Kirby-Bauer agar disc diffusion method. In addition, 64 E. coli isolates were tested for 6 colicin genes, fimH, hlyA, traT, csgA, crl virulence genes and bla−CTX-M-15, bla−oxa-2, and bla−oxa-10 resistance genes by polymerase chain reaction (PCR).Results: Extra-intestinal pathogenic E. coli isolated from urine and blood samples represented a battery of virulence factors and resistance genes with a great ability to produce biofilm. Also, a significant association (P< 0.05) among most of the tested colicin, virulence and resistance genes was observed. The observed associations indicate the importance and contribution of the tested factors in the establishment and the progress of infection especially with Extra-intestinal E. coli (ExPEC) which is considered a great challenging health problem.Conclusion: There is a need for studying how to control these factors to decrease the rate and the severity of infections. The relationship between virulence factors and resistance genes is complex and needs more studies that should be specific for each area.Keywords: E. coli, virulence, resistance, colicin genes, ESBL, bla−CTX-M-15, bla−oxa-2, bla−oxa-10https://www.dovepress.com/prevalence-of-virulence-genes-and-their-association-with-antimicrobial-peer-reviewed-article-IDRe. colivirulenceresistancecolicin genesesblbla-ctx-m-15bla-oxa-2and bla-oxa-10. |
| spellingShingle | Abd El-Baky RM Ibrahim RA Mohamed DS Ahmed EF Hashem ZS Prevalence of Virulence Genes and Their Association with Antimicrobial Resistance Among Pathogenic E. coli Isolated from Egyptian Patients with Different Clinical Infections Infection and Drug Resistance e. coli virulence resistance colicin genes esbl bla-ctx-m-15 bla-oxa-2 and bla-oxa-10. |
| title | Prevalence of Virulence Genes and Their Association with Antimicrobial Resistance Among Pathogenic E. coli Isolated from Egyptian Patients with Different Clinical Infections |
| title_full | Prevalence of Virulence Genes and Their Association with Antimicrobial Resistance Among Pathogenic E. coli Isolated from Egyptian Patients with Different Clinical Infections |
| title_fullStr | Prevalence of Virulence Genes and Their Association with Antimicrobial Resistance Among Pathogenic E. coli Isolated from Egyptian Patients with Different Clinical Infections |
| title_full_unstemmed | Prevalence of Virulence Genes and Their Association with Antimicrobial Resistance Among Pathogenic E. coli Isolated from Egyptian Patients with Different Clinical Infections |
| title_short | Prevalence of Virulence Genes and Their Association with Antimicrobial Resistance Among Pathogenic E. coli Isolated from Egyptian Patients with Different Clinical Infections |
| title_sort | prevalence of virulence genes and their association with antimicrobial resistance among pathogenic e coli isolated from egyptian patients with different clinical infections |
| topic | e. coli virulence resistance colicin genes esbl bla-ctx-m-15 bla-oxa-2 and bla-oxa-10. |
| url | https://www.dovepress.com/prevalence-of-virulence-genes-and-their-association-with-antimicrobial-peer-reviewed-article-IDR |
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