Validation of a simple and inexpensive method for the quantitation of infarct in the rat brain
A gravimetric method was evaluated as a simple, sensitive, reproducible, low-cost alternative to quantify the extent of brain infarct after occlusion of the medial cerebral artery in rats. In ether-anesthetized rats, the left medial cerebral artery was occluded for 1, 1.5 or 2 h by inserting a 4-0 n...
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| Format: | Article |
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Associação Brasileira de Divulgação Científica
2004-04-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400008 |
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| author | C.L.R. Schilichting K.C.M. Lima L.A. Cestari Junior J.Y. Sekiyama F.M. Silva H. Milani |
| author_facet | C.L.R. Schilichting K.C.M. Lima L.A. Cestari Junior J.Y. Sekiyama F.M. Silva H. Milani |
| author_sort | C.L.R. Schilichting |
| collection | DOAJ |
| description | A gravimetric method was evaluated as a simple, sensitive, reproducible, low-cost alternative to quantify the extent of brain infarct after occlusion of the medial cerebral artery in rats. In ether-anesthetized rats, the left medial cerebral artery was occluded for 1, 1.5 or 2 h by inserting a 4-0 nylon monofilament suture into the internal carotid artery. Twenty-four hours later, the brains were processed for histochemical triphenyltetrazolium chloride (TTC) staining and quantitation of the schemic infarct. In each TTC-stained brain section, the ischemic tissue was dissected with a scalpel and fixed in 10% formalin at 0ºC until its total mass could be estimated. The mass (mg) of the ischemic tissue was weighed on an analytical balance and compared to its volume (mm³), estimated either by plethysmometry using platinum electrodes or by computer-assisted image analysis. Infarct size as measured by the weighing method (mg), and reported as a percent (%) of the affected (left) hemisphere, correlated closely with volume (mm³, also reported as %) estimated by computerized image analysis (r = 0.88; P < 0.001; N = 10) or by plethysmography (r = 0.97-0.98; P < 0.0001; N = 41). This degree of correlation was maintained between different experimenters. The method was also sensitive for detecting the effect of different ischemia durations on infarct size (P < 0.005; N = 23), and the effect of drug treatments in reducing the extent of brain damage (P < 0.005; N = 24). The data suggest that, in addition to being simple and low cost, the weighing method is a reliable alternative for quantifying brain infarct in animal models of stroke. |
| first_indexed | 2024-04-13T04:55:11Z |
| format | Article |
| id | doaj.art-4d5e1fedeef14634b50e6210bb765d86 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-04-13T04:55:11Z |
| publishDate | 2004-04-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-4d5e1fedeef14634b50e6210bb765d862022-12-22T03:01:31ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2004-04-0137451152110.1590/S0100-879X2004000400008Validation of a simple and inexpensive method for the quantitation of infarct in the rat brainC.L.R. SchilichtingK.C.M. LimaL.A. Cestari JuniorJ.Y. SekiyamaF.M. SilvaH. MilaniA gravimetric method was evaluated as a simple, sensitive, reproducible, low-cost alternative to quantify the extent of brain infarct after occlusion of the medial cerebral artery in rats. In ether-anesthetized rats, the left medial cerebral artery was occluded for 1, 1.5 or 2 h by inserting a 4-0 nylon monofilament suture into the internal carotid artery. Twenty-four hours later, the brains were processed for histochemical triphenyltetrazolium chloride (TTC) staining and quantitation of the schemic infarct. In each TTC-stained brain section, the ischemic tissue was dissected with a scalpel and fixed in 10% formalin at 0ºC until its total mass could be estimated. The mass (mg) of the ischemic tissue was weighed on an analytical balance and compared to its volume (mm³), estimated either by plethysmometry using platinum electrodes or by computer-assisted image analysis. Infarct size as measured by the weighing method (mg), and reported as a percent (%) of the affected (left) hemisphere, correlated closely with volume (mm³, also reported as %) estimated by computerized image analysis (r = 0.88; P < 0.001; N = 10) or by plethysmography (r = 0.97-0.98; P < 0.0001; N = 41). This degree of correlation was maintained between different experimenters. The method was also sensitive for detecting the effect of different ischemia durations on infarct size (P < 0.005; N = 23), and the effect of drug treatments in reducing the extent of brain damage (P < 0.005; N = 24). The data suggest that, in addition to being simple and low cost, the weighing method is a reliable alternative for quantifying brain infarct in animal models of stroke.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400008Focal cerebral ischemiaInfarct sizeRatsTriphenyltetrazolium chloride stainWeighing method |
| spellingShingle | C.L.R. Schilichting K.C.M. Lima L.A. Cestari Junior J.Y. Sekiyama F.M. Silva H. Milani Validation of a simple and inexpensive method for the quantitation of infarct in the rat brain Brazilian Journal of Medical and Biological Research Focal cerebral ischemia Infarct size Rats Triphenyltetrazolium chloride stain Weighing method |
| title | Validation of a simple and inexpensive method for the quantitation of infarct in the rat brain |
| title_full | Validation of a simple and inexpensive method for the quantitation of infarct in the rat brain |
| title_fullStr | Validation of a simple and inexpensive method for the quantitation of infarct in the rat brain |
| title_full_unstemmed | Validation of a simple and inexpensive method for the quantitation of infarct in the rat brain |
| title_short | Validation of a simple and inexpensive method for the quantitation of infarct in the rat brain |
| title_sort | validation of a simple and inexpensive method for the quantitation of infarct in the rat brain |
| topic | Focal cerebral ischemia Infarct size Rats Triphenyltetrazolium chloride stain Weighing method |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400008 |
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