Tunable Double-Network GelMA/Alginate Hydrogels for Platelet Lysate-Derived Protein Delivery
Hydrogels (gels) are attractive tools for tissue engineering and regenerative medicine due to their potential for drug delivery and ECM-like composition. In this study, we use rheology to characterize GelMA/alginate gels loaded with human platelet lysate (PL). We then characterize these gels from a...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-09-01
|
| Series: | Bioengineering |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-5354/10/9/1044 |
| _version_ | 1827727140723884032 |
|---|---|
| author | Andrea Marfoglia Fahd Tibourtine Ludovic Pilloux Sophie Cazalbou |
| author_facet | Andrea Marfoglia Fahd Tibourtine Ludovic Pilloux Sophie Cazalbou |
| author_sort | Andrea Marfoglia |
| collection | DOAJ |
| description | Hydrogels (gels) are attractive tools for tissue engineering and regenerative medicine due to their potential for drug delivery and ECM-like composition. In this study, we use rheology to characterize GelMA/alginate gels loaded with human platelet lysate (PL). We then characterize these gels from a physicochemical perspective and evaluate their ability to transport PL proteins, their pore size, and their rate of degradation. Finally, their biocompatibility is evaluated. We describe how alginate changes the mechanical behavior of the gels from elastic to viscoelastic after ionic (calcium-mediated) crosslinking. In addition, we report the release of ~90% of PL proteins from the gels and relate it to the degradation profile of the gels. Finally, we evaluated the biocompatibility of the gels. Thus, the developed gels represent attractive substrates for both cell studies and as bioactive materials. |
| first_indexed | 2024-03-10T23:01:18Z |
| format | Article |
| id | doaj.art-4d635f552d084d03bfea71bc2749840c |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-10T23:01:18Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-4d635f552d084d03bfea71bc2749840c2023-11-19T09:36:54ZengMDPI AGBioengineering2306-53542023-09-01109104410.3390/bioengineering10091044Tunable Double-Network GelMA/Alginate Hydrogels for Platelet Lysate-Derived Protein DeliveryAndrea Marfoglia0Fahd Tibourtine1Ludovic Pilloux2Sophie Cazalbou3CIRIMAT, Université Toulouse 3 Paul Sabatier, Toulouse INP, CNRS, Université de Toulouse, 31062 Toulouse, FranceCIRIMAT, Université Toulouse 3 Paul Sabatier, Toulouse INP, CNRS, Université de Toulouse, 31062 Toulouse, FranceLaboratoire de Génie Chimique, Université Toulouse 3 Paul Sabatier, Toulouse INP, CNRS, Université de Toulouse, 31062 Toulouse, FranceCIRIMAT, Université Toulouse 3 Paul Sabatier, Toulouse INP, CNRS, Université de Toulouse, 31062 Toulouse, FranceHydrogels (gels) are attractive tools for tissue engineering and regenerative medicine due to their potential for drug delivery and ECM-like composition. In this study, we use rheology to characterize GelMA/alginate gels loaded with human platelet lysate (PL). We then characterize these gels from a physicochemical perspective and evaluate their ability to transport PL proteins, their pore size, and their rate of degradation. Finally, their biocompatibility is evaluated. We describe how alginate changes the mechanical behavior of the gels from elastic to viscoelastic after ionic (calcium-mediated) crosslinking. In addition, we report the release of ~90% of PL proteins from the gels and relate it to the degradation profile of the gels. Finally, we evaluated the biocompatibility of the gels. Thus, the developed gels represent attractive substrates for both cell studies and as bioactive materials.https://www.mdpi.com/2306-5354/10/9/1044tissue engineeringregenerative medicinehydrogelsGelMAalginateplatelet lysate |
| spellingShingle | Andrea Marfoglia Fahd Tibourtine Ludovic Pilloux Sophie Cazalbou Tunable Double-Network GelMA/Alginate Hydrogels for Platelet Lysate-Derived Protein Delivery Bioengineering tissue engineering regenerative medicine hydrogels GelMA alginate platelet lysate |
| title | Tunable Double-Network GelMA/Alginate Hydrogels for Platelet Lysate-Derived Protein Delivery |
| title_full | Tunable Double-Network GelMA/Alginate Hydrogels for Platelet Lysate-Derived Protein Delivery |
| title_fullStr | Tunable Double-Network GelMA/Alginate Hydrogels for Platelet Lysate-Derived Protein Delivery |
| title_full_unstemmed | Tunable Double-Network GelMA/Alginate Hydrogels for Platelet Lysate-Derived Protein Delivery |
| title_short | Tunable Double-Network GelMA/Alginate Hydrogels for Platelet Lysate-Derived Protein Delivery |
| title_sort | tunable double network gelma alginate hydrogels for platelet lysate derived protein delivery |
| topic | tissue engineering regenerative medicine hydrogels GelMA alginate platelet lysate |
| url | https://www.mdpi.com/2306-5354/10/9/1044 |
| work_keys_str_mv | AT andreamarfoglia tunabledoublenetworkgelmaalginatehydrogelsforplateletlysatederivedproteindelivery AT fahdtibourtine tunabledoublenetworkgelmaalginatehydrogelsforplateletlysatederivedproteindelivery AT ludovicpilloux tunabledoublenetworkgelmaalginatehydrogelsforplateletlysatederivedproteindelivery AT sophiecazalbou tunabledoublenetworkgelmaalginatehydrogelsforplateletlysatederivedproteindelivery |