High-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet
Abstract This paper proposes a simple, effective, non-scanning method for the visualization of a cell-attached nanointerface. The method uses localized surface plasmon resonance (LSPR) excited homogeneously on a two-dimensional (2D) self-assembled gold-nanoparticle sheet. The LSPR of the gold-nanopa...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-04000-4 |
| _version_ | 1818842472395046912 |
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| author | Shihomi Masuda Yuhki Yanase Eiji Usukura Sou Ryuzaki Pangpang Wang Koichi Okamoto Thasaneeya Kuboki Satoru Kidoaki Kaoru Tamada |
| author_facet | Shihomi Masuda Yuhki Yanase Eiji Usukura Sou Ryuzaki Pangpang Wang Koichi Okamoto Thasaneeya Kuboki Satoru Kidoaki Kaoru Tamada |
| author_sort | Shihomi Masuda |
| collection | DOAJ |
| description | Abstract This paper proposes a simple, effective, non-scanning method for the visualization of a cell-attached nanointerface. The method uses localized surface plasmon resonance (LSPR) excited homogeneously on a two-dimensional (2D) self-assembled gold-nanoparticle sheet. The LSPR of the gold-nanoparticle sheet provides high-contrast interfacial images due to the confined light within a region a few tens of nanometers from the particles and the enhancement of fluorescence. Test experiments on rat basophilic leukemia (RBL-2H3) cells with fluorescence-labeled actin filaments revealed high axial and lateral resolution even under a regular epifluorescence microscope, which produced higher quality images than those captured under a total internal reflection fluorescence (TIRF) microscope. This non-scanning-type, high-resolution imaging method will be an effective tool for monitoring interfacial phenomena that exhibit relatively rapid reaction kinetics in various cellular and molecular dynamics. |
| first_indexed | 2024-12-19T04:42:31Z |
| format | Article |
| id | doaj.art-4d642a9d2b364ff8a1cd247ec8e4257a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T04:42:31Z |
| publishDate | 2017-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-4d642a9d2b364ff8a1cd247ec8e4257a2022-12-21T20:35:34ZengNature PortfolioScientific Reports2045-23222017-06-017111010.1038/s41598-017-04000-4High-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheetShihomi Masuda0Yuhki Yanase1Eiji Usukura2Sou Ryuzaki3Pangpang Wang4Koichi Okamoto5Thasaneeya Kuboki6Satoru Kidoaki7Kaoru Tamada8Institute for Materials Chemistry and Engineering, Kyushu UniversityGraduate School of Biomedical & Health Science, Hiroshima UniversityGraduate School of Science, Nagoya UniversityInstitute for Materials Chemistry and Engineering, Kyushu UniversityEducation Center for Global Leaders in Molecular Systems for Devices, Kyushu UniversityInstitute for Materials Chemistry and Engineering, Kyushu UniversityInstitute for Materials Chemistry and Engineering, Kyushu UniversityInstitute for Materials Chemistry and Engineering, Kyushu UniversityInstitute for Materials Chemistry and Engineering, Kyushu UniversityAbstract This paper proposes a simple, effective, non-scanning method for the visualization of a cell-attached nanointerface. The method uses localized surface plasmon resonance (LSPR) excited homogeneously on a two-dimensional (2D) self-assembled gold-nanoparticle sheet. The LSPR of the gold-nanoparticle sheet provides high-contrast interfacial images due to the confined light within a region a few tens of nanometers from the particles and the enhancement of fluorescence. Test experiments on rat basophilic leukemia (RBL-2H3) cells with fluorescence-labeled actin filaments revealed high axial and lateral resolution even under a regular epifluorescence microscope, which produced higher quality images than those captured under a total internal reflection fluorescence (TIRF) microscope. This non-scanning-type, high-resolution imaging method will be an effective tool for monitoring interfacial phenomena that exhibit relatively rapid reaction kinetics in various cellular and molecular dynamics.https://doi.org/10.1038/s41598-017-04000-4 |
| spellingShingle | Shihomi Masuda Yuhki Yanase Eiji Usukura Sou Ryuzaki Pangpang Wang Koichi Okamoto Thasaneeya Kuboki Satoru Kidoaki Kaoru Tamada High-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet Scientific Reports |
| title | High-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet |
| title_full | High-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet |
| title_fullStr | High-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet |
| title_full_unstemmed | High-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet |
| title_short | High-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet |
| title_sort | high resolution imaging of a cell attached nanointerface using a gold nanoparticle two dimensional sheet |
| url | https://doi.org/10.1038/s41598-017-04000-4 |
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