Deoxyvasicinone with Anti-Melanogenic Activity from Marine-Derived <i>Streptomyces</i> sp. CNQ-617
The tricyclic quinazoline alkaloid deoxyvasicinone (<b>DOV</b>, <b>1</b>) was isolated from a marine-derived <i>Streptomyces</i> sp. CNQ-617, and its anti-melanogenic effects were investigated. Deoxyvasicinone was shown to decrease the melanin content of B16F10 an...
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MDPI AG
2022-02-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/20/2/155 |
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| author | Se-eun Lee Min-ju Kim Prima F. Hillman Dong-Chan Oh William Fenical Sang-Jip Nam Kyung-Min Lim |
| author_facet | Se-eun Lee Min-ju Kim Prima F. Hillman Dong-Chan Oh William Fenical Sang-Jip Nam Kyung-Min Lim |
| author_sort | Se-eun Lee |
| collection | DOAJ |
| description | The tricyclic quinazoline alkaloid deoxyvasicinone (<b>DOV</b>, <b>1</b>) was isolated from a marine-derived <i>Streptomyces</i> sp. CNQ-617, and its anti-melanogenic effects were investigated. Deoxyvasicinone was shown to decrease the melanin content of B16F10 and MNT-1 cells that have been stimulated by α-melanocyte-stimulating hormone (α-MSH). In addition, microscopic images of the cells showed that deoxyvasicinone attenuated melanocyte activation. Although, deoxyvasicinone did not directly inhibit tyrosinase (TYR) enzymatic activity, real-time PCR showed that it inhibited the mRNA expression of TYR, tyrosinase-related protein 1 (TRP-1), and tyrosinase-related protein 2 (TRP-2). In the artificial 3D pigmented skin model Melanoderm<sup>TM</sup>, deoxyvasicinone brightened the skin significantly, as confirmed by histological examination. In conclusion, this study demonstrated that the marine microbial natural product deoxyvascinone has an anti-melanogenic effect through downregulation of melanogenic enzymes. |
| first_indexed | 2024-03-09T21:33:16Z |
| format | Article |
| id | doaj.art-4d657bb51f6a486cba71426307c39fe6 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T21:33:16Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-4d657bb51f6a486cba71426307c39fe62023-11-23T20:50:42ZengMDPI AGMarine Drugs1660-33972022-02-0120215510.3390/md20020155Deoxyvasicinone with Anti-Melanogenic Activity from Marine-Derived <i>Streptomyces</i> sp. CNQ-617Se-eun Lee0Min-ju Kim1Prima F. Hillman2Dong-Chan Oh3William Fenical4Sang-Jip Nam5Kyung-Min Lim6The Graduate School of Industrial Pharmaceutical Sciences, Ewha Womans University, Seoul 03760, KoreaThe Graduate School of Industrial Pharmaceutical Sciences, Ewha Womans University, Seoul 03760, KoreaDepartment of Chemistry and Nanoscience, Ewha Womans University, Seoul 03760, KoreaNatural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, KoreaCenter for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, CA 92093-0204, USADepartment of Chemistry and Nanoscience, Ewha Womans University, Seoul 03760, KoreaCollege of Pharmacy, Ewha Womans University, Seoul 03760, KoreaThe tricyclic quinazoline alkaloid deoxyvasicinone (<b>DOV</b>, <b>1</b>) was isolated from a marine-derived <i>Streptomyces</i> sp. CNQ-617, and its anti-melanogenic effects were investigated. Deoxyvasicinone was shown to decrease the melanin content of B16F10 and MNT-1 cells that have been stimulated by α-melanocyte-stimulating hormone (α-MSH). In addition, microscopic images of the cells showed that deoxyvasicinone attenuated melanocyte activation. Although, deoxyvasicinone did not directly inhibit tyrosinase (TYR) enzymatic activity, real-time PCR showed that it inhibited the mRNA expression of TYR, tyrosinase-related protein 1 (TRP-1), and tyrosinase-related protein 2 (TRP-2). In the artificial 3D pigmented skin model Melanoderm<sup>TM</sup>, deoxyvasicinone brightened the skin significantly, as confirmed by histological examination. In conclusion, this study demonstrated that the marine microbial natural product deoxyvascinone has an anti-melanogenic effect through downregulation of melanogenic enzymes.https://www.mdpi.com/1660-3397/20/2/155<i>Streptomyces</i> sp.deoxyvasicinonemarine natural productsanti-melanogenic effect |
| spellingShingle | Se-eun Lee Min-ju Kim Prima F. Hillman Dong-Chan Oh William Fenical Sang-Jip Nam Kyung-Min Lim Deoxyvasicinone with Anti-Melanogenic Activity from Marine-Derived <i>Streptomyces</i> sp. CNQ-617 Marine Drugs <i>Streptomyces</i> sp. deoxyvasicinone marine natural products anti-melanogenic effect |
| title | Deoxyvasicinone with Anti-Melanogenic Activity from Marine-Derived <i>Streptomyces</i> sp. CNQ-617 |
| title_full | Deoxyvasicinone with Anti-Melanogenic Activity from Marine-Derived <i>Streptomyces</i> sp. CNQ-617 |
| title_fullStr | Deoxyvasicinone with Anti-Melanogenic Activity from Marine-Derived <i>Streptomyces</i> sp. CNQ-617 |
| title_full_unstemmed | Deoxyvasicinone with Anti-Melanogenic Activity from Marine-Derived <i>Streptomyces</i> sp. CNQ-617 |
| title_short | Deoxyvasicinone with Anti-Melanogenic Activity from Marine-Derived <i>Streptomyces</i> sp. CNQ-617 |
| title_sort | deoxyvasicinone with anti melanogenic activity from marine derived i streptomyces i sp cnq 617 |
| topic | <i>Streptomyces</i> sp. deoxyvasicinone marine natural products anti-melanogenic effect |
| url | https://www.mdpi.com/1660-3397/20/2/155 |
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