Effects of wasp venom on venous thrombosis in rats
Objective(s): This study aimed to investigate the potential effects of wasp venom (WV) from Vespa magnifica on antithrombosis in rats with inferior vena cava (IVC) thrombosis.Materials and Methods: The thrombosis rat model was established by improving the IVC stenosis, in which rats were subjected t...
| প্রধান লেখক: | , , , , , , , |
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| বিন্যাস: | প্রবন্ধ |
| ভাষা: | English |
| প্রকাশিত: |
Mashhad University of Medical Sciences
2022-07-01
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| মালা: | Iranian Journal of Basic Medical Sciences |
| বিষয়গুলি: | |
| অনলাইন ব্যবহার করুন: | https://ijbms.mums.ac.ir/article_20619_f484f6c3369003d137ebac5b79f3aaa1.pdf |
| _version_ | 1828159065480495104 |
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| author | Fan-mao Jin Mei Wang Xiumei Wu Huai Xiao Dexiao Wang Guang-ming Wang Cheng-gui Zhang Hai-rong Zhao |
| author_facet | Fan-mao Jin Mei Wang Xiumei Wu Huai Xiao Dexiao Wang Guang-ming Wang Cheng-gui Zhang Hai-rong Zhao |
| author_sort | Fan-mao Jin |
| collection | DOAJ |
| description | Objective(s): This study aimed to investigate the potential effects of wasp venom (WV) from Vespa magnifica on antithrombosis in rats with inferior vena cava (IVC) thrombosis.Materials and Methods: The thrombosis rat model was established by improving the IVC stenosis, in which rats were subjected to IVC ligation for 75 min. Rats were administered argatroban (IP) or WV (s.c.) for 4 hr after IVC thrombosis. The weight, inhibition rate, and pathological morphology of the thrombosis induced by IVC ligation and the variation in four coagulation parameters, coagulation factors, and CD61+CD62P+ were simultaneously determined in IVC rats.Results: The thrombus formed as a result of IVC ligation was stable. Compared with the control group, the weight of the thrombus was significantly reduced in the argatroban group. Thrombus weight was reduced by treatment with 0.6, 0.2, and 0.05 mg/kg WV, with inhibition rates of 52.19%, 35.32%, and 28.98%, respectively. Inflammatory cells adhered to and infiltrated the vessel wall in the IVC group more than in the sham group. However, the pathological morphology and CD61+CD62P+ of the WV treatment groups tended to be normal. Conclusion: We improved the model of IVC thrombosis to be suitable for evaluation of antithrombotic drugs. Our findings demonstrated that WV could inhibit IVC thrombosis associated with reducing coagulation factors V and CD61+CD62p expression in rats. |
| first_indexed | 2024-04-11T23:53:58Z |
| format | Article |
| id | doaj.art-4d6959203ee94b898d1d0043a05e446b |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-11T23:53:58Z |
| publishDate | 2022-07-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-4d6959203ee94b898d1d0043a05e446b2022-12-22T03:56:24ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742022-07-0125782282610.22038/ijbms.2022.63219.1396220619Effects of wasp venom on venous thrombosis in ratsFan-mao Jin0Mei Wang1Xiumei Wu2Huai Xiao3Dexiao Wang4Guang-ming Wang5Cheng-gui Zhang6Hai-rong Zhao7Lishui City People’s Hospital, Lishui, Zhejiang 323000, People’s Republic of ChinaYunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, ChinaYunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, ChinaYunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, ChinaYunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, ChinaGenetic Testing Center, The First Affiliated Hospital of Dali University, Dali University, Dali, ChinaYunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, ChinaYunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&D, Dali University, Dali, ChinaObjective(s): This study aimed to investigate the potential effects of wasp venom (WV) from Vespa magnifica on antithrombosis in rats with inferior vena cava (IVC) thrombosis.Materials and Methods: The thrombosis rat model was established by improving the IVC stenosis, in which rats were subjected to IVC ligation for 75 min. Rats were administered argatroban (IP) or WV (s.c.) for 4 hr after IVC thrombosis. The weight, inhibition rate, and pathological morphology of the thrombosis induced by IVC ligation and the variation in four coagulation parameters, coagulation factors, and CD61+CD62P+ were simultaneously determined in IVC rats.Results: The thrombus formed as a result of IVC ligation was stable. Compared with the control group, the weight of the thrombus was significantly reduced in the argatroban group. Thrombus weight was reduced by treatment with 0.6, 0.2, and 0.05 mg/kg WV, with inhibition rates of 52.19%, 35.32%, and 28.98%, respectively. Inflammatory cells adhered to and infiltrated the vessel wall in the IVC group more than in the sham group. However, the pathological morphology and CD61+CD62P+ of the WV treatment groups tended to be normal. Conclusion: We improved the model of IVC thrombosis to be suitable for evaluation of antithrombotic drugs. Our findings demonstrated that WV could inhibit IVC thrombosis associated with reducing coagulation factors V and CD61+CD62p expression in rats.https://ijbms.mums.ac.ir/article_20619_f484f6c3369003d137ebac5b79f3aaa1.pdfargatrobanblood coagulation factorsplatelet activationvenous thrombosiswv |
| spellingShingle | Fan-mao Jin Mei Wang Xiumei Wu Huai Xiao Dexiao Wang Guang-ming Wang Cheng-gui Zhang Hai-rong Zhao Effects of wasp venom on venous thrombosis in rats Iranian Journal of Basic Medical Sciences argatroban blood coagulation factors platelet activation venous thrombosis wv |
| title | Effects of wasp venom on venous thrombosis in rats |
| title_full | Effects of wasp venom on venous thrombosis in rats |
| title_fullStr | Effects of wasp venom on venous thrombosis in rats |
| title_full_unstemmed | Effects of wasp venom on venous thrombosis in rats |
| title_short | Effects of wasp venom on venous thrombosis in rats |
| title_sort | effects of wasp venom on venous thrombosis in rats |
| topic | argatroban blood coagulation factors platelet activation venous thrombosis wv |
| url | https://ijbms.mums.ac.ir/article_20619_f484f6c3369003d137ebac5b79f3aaa1.pdf |
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