The Potential of Rhodotorula graminis TISTR 5124 for Synthesis of Polyhydroxyalkanoate (PHA) by Limitation of a Phosphorus and Nitrogen Sourc
Polyhydroxyalkanoate (PHA) is one of the alternatively biodegradable plastics which can be synthesized from a particular micro-organism after the fermentation process, considering the optimization of nutrients. In this research, the yeast strain Rhodotorula graminis TISTR 5124 was selected to b...
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Format: | Article |
Language: | English |
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Universitas Indonesia
2016-12-01
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Series: | International Journal of Technology |
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Online Access: | http://ijtech.eng.ui.ac.id/article/view/308 |
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author | Duangkanok Tanangteerapong Patipan Prasathinpimai Wasita Suebthamma Khanita Kamwilaisak |
author_facet | Duangkanok Tanangteerapong Patipan Prasathinpimai Wasita Suebthamma Khanita Kamwilaisak |
author_sort | Duangkanok Tanangteerapong |
collection | DOAJ |
description | Polyhydroxyalkanoate (PHA) is one of the
alternatively biodegradable plastics which can be synthesized from a particular
micro-organism after the fermentation process, considering the optimization of nutrients. In this
research, the
yeast strain Rhodotorula
graminis TISTR 5124 was selected to be fermented with a carbon source in the standard nutrient in order to conduct a preliminarily study on the best conditions for this
yeast in PHA production. The growth
rate curve of yeast in the composition of imbalanced nutrients, i.e. the limitation
of phosphorus and nitrogen, was
also investigated and compared with another sample cultured in standard nutrients. Experimental results indicated that the
condition that gave the maximum growth rate of this yeast strain was a P-limited condition at 81 hours, whereby the
cell number of 3.1×109cells/mL was obtained and corresponded to the
optical density (OD) of 0.95 measured at a wavelength of 600 nm. The
synthesized PHA extracted from yeast cells after 81 hours of incubation was
examined by
Fourier transform infra-red (FT-IR) and nuclear magnetic resonance (1H NMR)
spectroscopy. The results indicated stretching vibrations similar to the
copolymer PHBV (or a PHA derivative). Maximum
PHA content of 54.4% was found in the P-limited condition which corresponded to a
PHA yield of 65.1 (g/g-total sugar consumed) in which the yeast consumed the least glucose amount of 3.2 g/L, but grew the most rapidly. Rhodotorula graminis TISTR 5124 is
therefore promising as a good candidate for alternatively biodegradable plastics, considering the
potential to produce PHA and its derivatives. This process can be beneficial as an option to replace
conventional plastics in the future. |
first_indexed | 2024-04-11T03:36:31Z |
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institution | Directory Open Access Journal |
issn | 2086-9614 2087-2100 |
language | English |
last_indexed | 2024-04-11T03:36:31Z |
publishDate | 2016-12-01 |
publisher | Universitas Indonesia |
record_format | Article |
series | International Journal of Technology |
spelling | doaj.art-4d6a18e88bb84438bb527b559e79c74e2023-01-02T04:58:20ZengUniversitas IndonesiaInternational Journal of Technology2086-96142087-21002016-12-01771214122110.14716/ijtech.v7i7.308308The Potential of Rhodotorula graminis TISTR 5124 for Synthesis of Polyhydroxyalkanoate (PHA) by Limitation of a Phosphorus and Nitrogen SourcDuangkanok Tanangteerapong0Patipan Prasathinpimai1Wasita Suebthamma2Khanita Kamwilaisak3Department of Chemical Engineering, Faculty of Engineering, Khon Kaen University, Khon Kaen, 40002, ThailandDepartment of Chemical Engineering, Faculty of Engineering, Khon Kaen University, Khon Kaen, 40002, ThailandDepartment of Chemical Engineering, Faculty of Engineering, Khon Kaen University, Khon Kaen, 40002, ThailandDepartment of Chemical Engineering, Faculty of Engineering, Khon Kaen University, Khon Kaen, 40002, ThailandPolyhydroxyalkanoate (PHA) is one of the alternatively biodegradable plastics which can be synthesized from a particular micro-organism after the fermentation process, considering the optimization of nutrients. In this research, the yeast strain Rhodotorula graminis TISTR 5124 was selected to be fermented with a carbon source in the standard nutrient in order to conduct a preliminarily study on the best conditions for this yeast in PHA production. The growth rate curve of yeast in the composition of imbalanced nutrients, i.e. the limitation of phosphorus and nitrogen, was also investigated and compared with another sample cultured in standard nutrients. Experimental results indicated that the condition that gave the maximum growth rate of this yeast strain was a P-limited condition at 81 hours, whereby the cell number of 3.1×109cells/mL was obtained and corresponded to the optical density (OD) of 0.95 measured at a wavelength of 600 nm. The synthesized PHA extracted from yeast cells after 81 hours of incubation was examined by Fourier transform infra-red (FT-IR) and nuclear magnetic resonance (1H NMR) spectroscopy. The results indicated stretching vibrations similar to the copolymer PHBV (or a PHA derivative). Maximum PHA content of 54.4% was found in the P-limited condition which corresponded to a PHA yield of 65.1 (g/g-total sugar consumed) in which the yeast consumed the least glucose amount of 3.2 g/L, but grew the most rapidly. Rhodotorula graminis TISTR 5124 is therefore promising as a good candidate for alternatively biodegradable plastics, considering the potential to produce PHA and its derivatives. This process can be beneficial as an option to replace conventional plastics in the future.http://ijtech.eng.ui.ac.id/article/view/308BioplasticExtractionPolyhydroxyalkanoateRhodotorula graminis |
spellingShingle | Duangkanok Tanangteerapong Patipan Prasathinpimai Wasita Suebthamma Khanita Kamwilaisak The Potential of Rhodotorula graminis TISTR 5124 for Synthesis of Polyhydroxyalkanoate (PHA) by Limitation of a Phosphorus and Nitrogen Sourc International Journal of Technology Bioplastic Extraction Polyhydroxyalkanoate Rhodotorula graminis |
title | The Potential of Rhodotorula graminis TISTR 5124 for Synthesis of Polyhydroxyalkanoate (PHA) by Limitation of a Phosphorus and Nitrogen Sourc |
title_full | The Potential of Rhodotorula graminis TISTR 5124 for Synthesis of Polyhydroxyalkanoate (PHA) by Limitation of a Phosphorus and Nitrogen Sourc |
title_fullStr | The Potential of Rhodotorula graminis TISTR 5124 for Synthesis of Polyhydroxyalkanoate (PHA) by Limitation of a Phosphorus and Nitrogen Sourc |
title_full_unstemmed | The Potential of Rhodotorula graminis TISTR 5124 for Synthesis of Polyhydroxyalkanoate (PHA) by Limitation of a Phosphorus and Nitrogen Sourc |
title_short | The Potential of Rhodotorula graminis TISTR 5124 for Synthesis of Polyhydroxyalkanoate (PHA) by Limitation of a Phosphorus and Nitrogen Sourc |
title_sort | potential of rhodotorula graminis tistr 5124 for synthesis of polyhydroxyalkanoate pha by limitation of a phosphorus and nitrogen sourc |
topic | Bioplastic Extraction Polyhydroxyalkanoate Rhodotorula graminis |
url | http://ijtech.eng.ui.ac.id/article/view/308 |
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