| Summary: | Yukang Dong,1,* Yingying He,1,* Xia Zhou,1 Xia Lv,1 Jia Huang,1 Yaqi Li,1 Xin Qian,2 Fangfang Hu,3 Jiaying Zhu1 1Department of Emergency, Guizhou Provincial People’s Hospital, Guiyang, People’s Republic of China; 2Department of Pharmacy, Guizhou Provincial People’s Hospital, Guiyang, People’s Republic of China; 3Department of Clinical Laboratory, Guizhou Provincial People’s Hospital, Guiyang, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jiaying Zhu, Email miraclezhujy@163.comBackground: Mycoplasma hominis meningitis is a rare postoperative complication of neurosurgery. Accurate and early diagnosis of M. hominis remains challenging because of the limitations of traditional detection methods. Metagenomic next-generation sequencing (mNGS) is an advanced technique with high sensitivity and specificity for identifying infectious pathogens; however, its application in diagnosing M. hominis meningitis has not been widely studied.Case Presentation: We report the case of a 61-year-old man who presented with fever and headache after neurosurgical treatment for a cerebral hemorrhage. Empiric antibiotic therapy was ineffective. Traditional culture of pathogens and serological testing yielded negative results, but M. hominis was detected in the cerebrospinal fluid by mNGS. After further verification by polymerase chain reaction (PCR), the patient’s clinical treatment was adjusted accordingly. With targeted antibiotic intervention, the patient’s symptoms were effectively alleviated, and clinical indicators returned to normal levels. Furthermore, the abundance of M. hominis decreased significantly compared to the initial mNGS reading after targeted treatment, indicating that the infection caused by M. hominis was effectively controlled.Conclusion: Using mNGS, we found that M. hominis may be a candidate causative agent of meningitis. The technique also has the advantage of timeliness and accuracy that traditional cultures cannot achieve. A combination of mNGS with PCR is recommended to identify pathogens in the early stages of infectious diseases to administer targeted clinical medication.Keywords: Mycoplasma hominis, meningitis, metagenomic next-generation sequencing, cerebrospinal fluid, case report
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