The Relationship between Complement Components <i>C1R</i> and <i>C5</i> Gene Polymorphism and the Values of Blood Indices in Suckling Piglets
The main mechanism of innate immunity is the complement system. Its components include the protein products of the <i>C1R</i> and <i>C5</i> genes, which are involved in the classical activation pathway as well as the inflammatory and cytolytic immune responses, respectively....
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MDPI AG
2023-10-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/11/2015 |
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| author | Hanna Szymańska Ewa Dzika Tadeusz Jarosław Zabolewicz Krystyna Życzko |
| author_facet | Hanna Szymańska Ewa Dzika Tadeusz Jarosław Zabolewicz Krystyna Życzko |
| author_sort | Hanna Szymańska |
| collection | DOAJ |
| description | The main mechanism of innate immunity is the complement system. Its components include the protein products of the <i>C1R</i> and <i>C5</i> genes, which are involved in the classical activation pathway as well as the inflammatory and cytolytic immune responses, respectively. The aim of this study was to determine the relationship between PCR-restriction fragment length polymorphism in <i>C1R</i> (726T > C) and C5 (1044A > C) genes, and the values of hematological and biochemical blood indices in suckling crossbred (Polish Large White × Polish Landrace × Duroc × Pietrain) piglets (n = 473), considering their age (younger, 21 ± 3 days, n = 274; older, 35 ± 3 days, n = 199) and health status. The frequencies of the <i>C5</i> genotypes deviated from the Hardy–Weinberg expectations. Younger piglets, healthy piglets, piglets that deviated from physiological norms and older piglets with the <i>C1R</i> TT genotype all had lower white and red blood cell indices. In piglets with the <i>C5</i> CC genotype, younger piglets, piglets that deviated from physiological norms and older piglets, a greater number and/or percentage of monocytes were recorded in the blood. Older piglets also showed an increase in the number of leukocytes and granulocytes, along with a tendency for a decrease in the percentage of lymphocytes in their blood. We concluded that a polymorphism in the <i>C1R</i> gene may exhibit a functional association or genetic linkage with other genes involved in the process of erythropoiesis. Furthermore the relationship between the <i>C5</i> gene polymorphism and the number and/or percentage of monocytes in the blood may modify the body’s defense abilities. Piglets with the CC genotype, having an increased number/proportion of these cells in their blood, probably display a weakened immune response to pathogens or a chronic stimulation of the immune system. |
| first_indexed | 2024-03-09T16:47:46Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T16:47:46Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-4d6e9d36ea724c7bad59126fec10ac8c2023-11-24T14:43:44ZengMDPI AGGenes2073-44252023-10-011411201510.3390/genes14112015The Relationship between Complement Components <i>C1R</i> and <i>C5</i> Gene Polymorphism and the Values of Blood Indices in Suckling PigletsHanna Szymańska0Ewa Dzika1Tadeusz Jarosław Zabolewicz2Krystyna Życzko3Department of Medical Biology, School of Public Health, Collegium Medicum, University of Warmia and Mazury in Olsztyn, Żołnierska 14C, 10-561 Olsztyn, PolandDepartment of Medical Biology, School of Public Health, Collegium Medicum, University of Warmia and Mazury in Olsztyn, Żołnierska 14C, 10-561 Olsztyn, PolandDepartment of Animal Genetics, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-719 Olsztyn, PolandDepartment of Animal Genetics, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-719 Olsztyn, PolandThe main mechanism of innate immunity is the complement system. Its components include the protein products of the <i>C1R</i> and <i>C5</i> genes, which are involved in the classical activation pathway as well as the inflammatory and cytolytic immune responses, respectively. The aim of this study was to determine the relationship between PCR-restriction fragment length polymorphism in <i>C1R</i> (726T > C) and C5 (1044A > C) genes, and the values of hematological and biochemical blood indices in suckling crossbred (Polish Large White × Polish Landrace × Duroc × Pietrain) piglets (n = 473), considering their age (younger, 21 ± 3 days, n = 274; older, 35 ± 3 days, n = 199) and health status. The frequencies of the <i>C5</i> genotypes deviated from the Hardy–Weinberg expectations. Younger piglets, healthy piglets, piglets that deviated from physiological norms and older piglets with the <i>C1R</i> TT genotype all had lower white and red blood cell indices. In piglets with the <i>C5</i> CC genotype, younger piglets, piglets that deviated from physiological norms and older piglets, a greater number and/or percentage of monocytes were recorded in the blood. Older piglets also showed an increase in the number of leukocytes and granulocytes, along with a tendency for a decrease in the percentage of lymphocytes in their blood. We concluded that a polymorphism in the <i>C1R</i> gene may exhibit a functional association or genetic linkage with other genes involved in the process of erythropoiesis. Furthermore the relationship between the <i>C5</i> gene polymorphism and the number and/or percentage of monocytes in the blood may modify the body’s defense abilities. Piglets with the CC genotype, having an increased number/proportion of these cells in their blood, probably display a weakened immune response to pathogens or a chronic stimulation of the immune system.https://www.mdpi.com/2073-4425/14/11/2015<i>C1R</i><i>C5</i>complement componentsgene polymorphismblood indicespiglets |
| spellingShingle | Hanna Szymańska Ewa Dzika Tadeusz Jarosław Zabolewicz Krystyna Życzko The Relationship between Complement Components <i>C1R</i> and <i>C5</i> Gene Polymorphism and the Values of Blood Indices in Suckling Piglets Genes <i>C1R</i> <i>C5</i> complement components gene polymorphism blood indices piglets |
| title | The Relationship between Complement Components <i>C1R</i> and <i>C5</i> Gene Polymorphism and the Values of Blood Indices in Suckling Piglets |
| title_full | The Relationship between Complement Components <i>C1R</i> and <i>C5</i> Gene Polymorphism and the Values of Blood Indices in Suckling Piglets |
| title_fullStr | The Relationship between Complement Components <i>C1R</i> and <i>C5</i> Gene Polymorphism and the Values of Blood Indices in Suckling Piglets |
| title_full_unstemmed | The Relationship between Complement Components <i>C1R</i> and <i>C5</i> Gene Polymorphism and the Values of Blood Indices in Suckling Piglets |
| title_short | The Relationship between Complement Components <i>C1R</i> and <i>C5</i> Gene Polymorphism and the Values of Blood Indices in Suckling Piglets |
| title_sort | relationship between complement components i c1r i and i c5 i gene polymorphism and the values of blood indices in suckling piglets |
| topic | <i>C1R</i> <i>C5</i> complement components gene polymorphism blood indices piglets |
| url | https://www.mdpi.com/2073-4425/14/11/2015 |
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