Cloning of Two Nicotinic Acetylcholine Receptor Genes from Bemisia tabaci (Gennadius)
Nicotinic acetylcholine receptors (nAChRs) mediate fast cholinergic synaptic transmission in the insect nervous system. Neonicotinoid insecticides exhibit insecticidal activities by targeting these receptors. The aim of this study was to isolate cDNA clones of nAChR subunit genes of Bemisia tabaci (...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Turkish Science and Technology Publishing (TURSTEP)
2020-11-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
| Subjects: | |
| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/3291 |
| _version_ | 1797916561902665728 |
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| author | Gül Satar Mehmet Rifat Ulusoy Ke Dong |
| author_facet | Gül Satar Mehmet Rifat Ulusoy Ke Dong |
| author_sort | Gül Satar |
| collection | DOAJ |
| description | Nicotinic acetylcholine receptors (nAChRs) mediate fast cholinergic synaptic transmission in the insect nervous system. Neonicotinoid insecticides exhibit insecticidal activities by targeting these receptors. The aim of this study was to isolate cDNA clones of nAChR subunit genes of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). RACEs (Rapid Amplification of cDNA Ends) were applied to obtain partial-length cDNA sequences. We identified two partial cDNA clones encoding β1 and α8 subunit genes (Btβ1-1168 bp and Btα8-755 bp), respectively, from Bemisia tabaci (Bt). This is the first report of isolation of α8 from B. tabaci by cloning. Btβ1 and Btα8 possess characteristics that are typical of nAChR subunits. Phylogenetic analysis showed that Btβ1 and Btα8 clustered with the orthologous genes of other insect species. |
| first_indexed | 2024-04-10T12:59:19Z |
| format | Article |
| id | doaj.art-4d6eb8373c314b00bd4e0209b212803e |
| institution | Directory Open Access Journal |
| issn | 2148-127X |
| language | English |
| last_indexed | 2024-04-10T12:59:19Z |
| publishDate | 2020-11-01 |
| publisher | Turkish Science and Technology Publishing (TURSTEP) |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj.art-4d6eb8373c314b00bd4e0209b212803e2023-02-15T16:13:17ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2020-11-018112322232910.24925/turjaf.v8i11.2322-2329.32911832Cloning of Two Nicotinic Acetylcholine Receptor Genes from Bemisia tabaci (Gennadius)Gül Satar0Mehmet Rifat Ulusoy1Ke Dong2Biotechnology Research and Application Centre, Cukurova University, 01330 AdanaDepartment of Plant Protection, Cukurova University, 01330AdanaDepartment of Biology, Duke University, Durham, North CarolinaNicotinic acetylcholine receptors (nAChRs) mediate fast cholinergic synaptic transmission in the insect nervous system. Neonicotinoid insecticides exhibit insecticidal activities by targeting these receptors. The aim of this study was to isolate cDNA clones of nAChR subunit genes of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). RACEs (Rapid Amplification of cDNA Ends) were applied to obtain partial-length cDNA sequences. We identified two partial cDNA clones encoding β1 and α8 subunit genes (Btβ1-1168 bp and Btα8-755 bp), respectively, from Bemisia tabaci (Bt). This is the first report of isolation of α8 from B. tabaci by cloning. Btβ1 and Btα8 possess characteristics that are typical of nAChR subunits. Phylogenetic analysis showed that Btβ1 and Btα8 clustered with the orthologous genes of other insect species.http://www.agrifoodscience.com/index.php/TURJAF/article/view/3291cotton whiteflynachrneonicotinoidphylogenetic analysesrace |
| spellingShingle | Gül Satar Mehmet Rifat Ulusoy Ke Dong Cloning of Two Nicotinic Acetylcholine Receptor Genes from Bemisia tabaci (Gennadius) Turkish Journal of Agriculture: Food Science and Technology cotton whitefly nachr neonicotinoid phylogenetic analyses race |
| title | Cloning of Two Nicotinic Acetylcholine Receptor Genes from Bemisia tabaci (Gennadius) |
| title_full | Cloning of Two Nicotinic Acetylcholine Receptor Genes from Bemisia tabaci (Gennadius) |
| title_fullStr | Cloning of Two Nicotinic Acetylcholine Receptor Genes from Bemisia tabaci (Gennadius) |
| title_full_unstemmed | Cloning of Two Nicotinic Acetylcholine Receptor Genes from Bemisia tabaci (Gennadius) |
| title_short | Cloning of Two Nicotinic Acetylcholine Receptor Genes from Bemisia tabaci (Gennadius) |
| title_sort | cloning of two nicotinic acetylcholine receptor genes from bemisia tabaci gennadius |
| topic | cotton whitefly nachr neonicotinoid phylogenetic analyses race |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/3291 |
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