New red blood cell and reticulocyte parameters and reference values for healthy individuals and in chronic kidney disease
Introduction: The importance of local references values has been well described in the literature; this is because the characteristics of the population may influence the laboratory tests. Objective: To establish the reference range for traditional and extended red blood cell parameters and reticulo...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Patologia Clínica
2015-04-01
|
| Series: | Jornal Brasileiro de Patologia e Medicina Laboratorial |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442015000200077&lng=en&tlng=en |
| _version_ | 1819262655944196096 |
|---|---|
| author | Patrícia S. Scherer Daniela Moraes Terezinha P. Munhoz Vanessa Sgnaolin |
| author_facet | Patrícia S. Scherer Daniela Moraes Terezinha P. Munhoz Vanessa Sgnaolin |
| author_sort | Patrícia S. Scherer |
| collection | DOAJ |
| description | Introduction: The importance of local references values has been well described in the literature; this is because the characteristics of the population may influence the laboratory tests. Objective: To establish the reference range for traditional and extended red blood cell parameters and reticulocyte indices in order to investigate its application in patients with chronic kidney disease (CKD). Materials and methods: 249 blood donors (125 males and 124 females) were selected to establish the reference values. The hemodialysis sample consisted of 62 patients with terminal CKD (48 male and 14 female). All analyzes were performed using the Sysmex XE-5000 automated hematology analyzer. Results: Differences between reference values was observed in relation to gender: red blood cells (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular hemoglobin concentration (MCHC), percentage of hyperchromic red blood cells (%HYPER), percentage of microcytosis (%MICRO), percentage of macrocytosis (%MACRO), absolute reticulocyte count (RET), reticulocyte hemoglobin content (RET-He), immature reticulocyte fraction (IFR), low fluorescence reticulocytes (LFR), medium fluorescence reticulocytes (MFR), and high fluorescence reticulocytes (HFR). Individuals with CKD presented RBC, HGB, HCT, MCHC, red cell distribution width expressed as coefficient of variation (RDW-CV), percentage of hypochromic red blood cells (%HYPO), percentage of reticulocytes (RET%), RET (female group), IFR, LFR, MFR, and HFR results compatible with the anemic state, which can be observed in 91.8% of patients. All studied parameters were in the area under the curve (AUC) > 0.4. In male group, %HYPO (AUC: 0.806) and IFR (AUC: 0.762) presented higher AUC values, while female group presented %HYPO (AUC: 0.806), %HYPER (AUC: 0.815), and IFR (AUC: 0.660). Conclusion: The medical advancement, the development of new techniques and hematological parameters have revealed important information about functional integrity of bone marrow, diagnosis of anemia and recombinant human erythropoietin monitoring therapy used in hemodialysis patients. |
| first_indexed | 2024-12-23T20:01:09Z |
| format | Article |
| id | doaj.art-4d6f81562f4e47efbe901c3b2c54c78b |
| institution | Directory Open Access Journal |
| issn | 1678-4774 |
| language | English |
| last_indexed | 2024-12-23T20:01:09Z |
| publishDate | 2015-04-01 |
| publisher | Sociedade Brasileira de Patologia Clínica |
| record_format | Article |
| series | Jornal Brasileiro de Patologia e Medicina Laboratorial |
| spelling | doaj.art-4d6f81562f4e47efbe901c3b2c54c78b2022-12-21T17:33:05ZengSociedade Brasileira de Patologia ClínicaJornal Brasileiro de Patologia e Medicina Laboratorial1678-47742015-04-01512778410.5935/1676-2444.20150014S1676-24442015000200077New red blood cell and reticulocyte parameters and reference values for healthy individuals and in chronic kidney diseasePatrícia S. SchererDaniela MoraesTerezinha P. MunhozVanessa SgnaolinIntroduction: The importance of local references values has been well described in the literature; this is because the characteristics of the population may influence the laboratory tests. Objective: To establish the reference range for traditional and extended red blood cell parameters and reticulocyte indices in order to investigate its application in patients with chronic kidney disease (CKD). Materials and methods: 249 blood donors (125 males and 124 females) were selected to establish the reference values. The hemodialysis sample consisted of 62 patients with terminal CKD (48 male and 14 female). All analyzes were performed using the Sysmex XE-5000 automated hematology analyzer. Results: Differences between reference values was observed in relation to gender: red blood cells (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular hemoglobin concentration (MCHC), percentage of hyperchromic red blood cells (%HYPER), percentage of microcytosis (%MICRO), percentage of macrocytosis (%MACRO), absolute reticulocyte count (RET), reticulocyte hemoglobin content (RET-He), immature reticulocyte fraction (IFR), low fluorescence reticulocytes (LFR), medium fluorescence reticulocytes (MFR), and high fluorescence reticulocytes (HFR). Individuals with CKD presented RBC, HGB, HCT, MCHC, red cell distribution width expressed as coefficient of variation (RDW-CV), percentage of hypochromic red blood cells (%HYPO), percentage of reticulocytes (RET%), RET (female group), IFR, LFR, MFR, and HFR results compatible with the anemic state, which can be observed in 91.8% of patients. All studied parameters were in the area under the curve (AUC) > 0.4. In male group, %HYPO (AUC: 0.806) and IFR (AUC: 0.762) presented higher AUC values, while female group presented %HYPO (AUC: 0.806), %HYPER (AUC: 0.815), and IFR (AUC: 0.660). Conclusion: The medical advancement, the development of new techniques and hematological parameters have revealed important information about functional integrity of bone marrow, diagnosis of anemia and recombinant human erythropoietin monitoring therapy used in hemodialysis patients.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442015000200077&lng=en&tlng=envalores de referênciaíndices de eritrócitosreticulócitosinsuficiência renal crônica |
| spellingShingle | Patrícia S. Scherer Daniela Moraes Terezinha P. Munhoz Vanessa Sgnaolin New red blood cell and reticulocyte parameters and reference values for healthy individuals and in chronic kidney disease Jornal Brasileiro de Patologia e Medicina Laboratorial valores de referência índices de eritrócitos reticulócitos insuficiência renal crônica |
| title | New red blood cell and reticulocyte parameters and reference values for healthy individuals and in chronic kidney disease |
| title_full | New red blood cell and reticulocyte parameters and reference values for healthy individuals and in chronic kidney disease |
| title_fullStr | New red blood cell and reticulocyte parameters and reference values for healthy individuals and in chronic kidney disease |
| title_full_unstemmed | New red blood cell and reticulocyte parameters and reference values for healthy individuals and in chronic kidney disease |
| title_short | New red blood cell and reticulocyte parameters and reference values for healthy individuals and in chronic kidney disease |
| title_sort | new red blood cell and reticulocyte parameters and reference values for healthy individuals and in chronic kidney disease |
| topic | valores de referência índices de eritrócitos reticulócitos insuficiência renal crônica |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442015000200077&lng=en&tlng=en |
| work_keys_str_mv | AT patriciasscherer newredbloodcellandreticulocyteparametersandreferencevaluesforhealthyindividualsandinchronickidneydisease AT danielamoraes newredbloodcellandreticulocyteparametersandreferencevaluesforhealthyindividualsandinchronickidneydisease AT terezinhapmunhoz newredbloodcellandreticulocyteparametersandreferencevaluesforhealthyindividualsandinchronickidneydisease AT vanessasgnaolin newredbloodcellandreticulocyteparametersandreferencevaluesforhealthyindividualsandinchronickidneydisease |