A static magnetic field enhances the repair of osteoarthritic cartilage by promoting the migration of stem cells and chondrogenesis
Objective: To investigate the therapeutic effects of static magnetic field (SMF) and its regulatory mechanism in the repair of osteoarthritic cartilage. Methods: Fourteen-week-old female C57BL/6 mice were randomly divided into the sham operation group and the osteoarthritis (OA) groups with and with...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-03-01
|
| Series: | Journal of Orthopaedic Translation |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214031X2200136X |
| _version_ | 1797837087629639680 |
|---|---|
| author | Yuting Sun Yanwen Fang Xinle Li Jie Li Daquan Liu Min Wei Zhongcai Liao Yao Meng Lidong Zhai Hiroki Yokota Lei Yang Ying Yu Ping Zhang |
| author_facet | Yuting Sun Yanwen Fang Xinle Li Jie Li Daquan Liu Min Wei Zhongcai Liao Yao Meng Lidong Zhai Hiroki Yokota Lei Yang Ying Yu Ping Zhang |
| author_sort | Yuting Sun |
| collection | DOAJ |
| description | Objective: To investigate the therapeutic effects of static magnetic field (SMF) and its regulatory mechanism in the repair of osteoarthritic cartilage. Methods: Fourteen-week-old female C57BL/6 mice were randomly divided into the sham operation group and the osteoarthritis (OA) groups with and without SMF application. SMF was applied at 200 mT for two consecutive weeks. Changes in knee cartilage were examined by histomorphometry, and the chondrogenesis and migration of endogenous stem cells were assessed. The expression of SRY-related protein 9 (SOX9), Collagen type II (COL2), matrix metallopeptidase 13 (MMP13), stromal cell-derived factor 1/C-X-C chemokine receptor type 4 (SDF-1/CXCR4), Piezo1 and other genes was evaluated, and the mechanism of SMF's action was tested using the CXCR4 inhibitor, AMD3100, and Piezo1 siRNA. Results: SMF significantly decreased the OARSI scores after induction of OA. SMF was beneficial to chondrogenesis by elevating SOX9. In the OA mouse model, an increase in MMP13 with a decrease in COL2 led to the destruction of the cartilage extracellular matrix, which was suppressed by SMF. SMF promoted the migration of cartilage-derived stem/progenitor cells and bone marrow-derived mesenchymal stem cells (MSCs). It increased SDF-1 and CXCR4, while the CXCR4 inhibitor significantly suppressed the beneficial effects of SMF. The application of Piezo1 siRNA inhibited the SMF-induced increase of CXCR4. Conclusion: SMF enhanced chondrogenesis and improved cartilage extracellular matrices. It activated the Piezo1-mediated SDF-1/CXCR4 regulatory axis and promoted the migration of endogenous stem cells. Collectively, it attenuated the pathological progression of cartilage destruction in OA mice. The Translational potential of this article: The findings in this study provided convincing evidence that SMF could enhance cartilage repair and improve OA symptoms, suggesting that SMF could have clinical value in the treatment of OA. |
| first_indexed | 2024-04-09T15:20:16Z |
| format | Article |
| id | doaj.art-4d711a1b008a43e38c900f5a089c7f06 |
| institution | Directory Open Access Journal |
| issn | 2214-031X |
| language | English |
| last_indexed | 2024-04-09T15:20:16Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Orthopaedic Translation |
| spelling | doaj.art-4d711a1b008a43e38c900f5a089c7f062023-04-29T14:49:44ZengElsevierJournal of Orthopaedic Translation2214-031X2023-03-01394354A static magnetic field enhances the repair of osteoarthritic cartilage by promoting the migration of stem cells and chondrogenesisYuting Sun0Yanwen Fang1Xinle Li2Jie Li3Daquan Liu4Min Wei5Zhongcai Liao6Yao Meng7Lidong Zhai8Hiroki Yokota9Lei Yang10Ying Yu11Ping Zhang12Department of Anatomy and Histology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, ChinaHeye Health Technology Co., Ltd., Huzhou, ChinaDepartment of Anatomy and Histology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China; Key Laboratory of Hormones and Development (Ministry of Health), Tianjin Key Laboratory of Metabolic Diseases, Tianjin Medical University, Tianjin, ChinaDepartment of Anatomy and Histology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China; Key Laboratory of Hormones and Development (Ministry of Health), Tianjin Key Laboratory of Metabolic Diseases, Tianjin Medical University, Tianjin, ChinaDepartment of Anatomy and Histology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China; Key Laboratory of Hormones and Development (Ministry of Health), Tianjin Key Laboratory of Metabolic Diseases, Tianjin Medical University, Tianjin, ChinaHeye Health Technology Co., Ltd., Huzhou, ChinaHeye Health Technology Co., Ltd., Huzhou, ChinaDepartment of Anatomy and Histology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, ChinaDepartment of Anatomy and Histology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, ChinaDepartment of Biomedical Engineering, Indiana University-Purdue University Indianapolis, IN, USACenter for Health Sciences and Engineering, Hebei Key Laboratory of Biomaterials and Smart Theranostics, School of Health Sciences and Biomedical Engineering, Hebei University of Technology, Tianjin, ChinaDepartment of Pharmacology, Tianjin Key Laboratory of Inflammatory Biology, The Province and Ministry Co-sponsored Collaborative Innovation Center for Medical Epigenetics, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, ChinaDepartment of Anatomy and Histology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China; Key Laboratory of Hormones and Development (Ministry of Health), Tianjin Key Laboratory of Metabolic Diseases, Tianjin Medical University, Tianjin, China; Tianjin Key Laboratory of Spine and Spinal Cord, Tianjin Medical University, Tianjin, China; Corresponding author. Department of Anatomy and Histology School of Basic Medical Sciences Tianjin Medical University, 22 Qixiangtai Road, Tianjin, 300070, China.Objective: To investigate the therapeutic effects of static magnetic field (SMF) and its regulatory mechanism in the repair of osteoarthritic cartilage. Methods: Fourteen-week-old female C57BL/6 mice were randomly divided into the sham operation group and the osteoarthritis (OA) groups with and without SMF application. SMF was applied at 200 mT for two consecutive weeks. Changes in knee cartilage were examined by histomorphometry, and the chondrogenesis and migration of endogenous stem cells were assessed. The expression of SRY-related protein 9 (SOX9), Collagen type II (COL2), matrix metallopeptidase 13 (MMP13), stromal cell-derived factor 1/C-X-C chemokine receptor type 4 (SDF-1/CXCR4), Piezo1 and other genes was evaluated, and the mechanism of SMF's action was tested using the CXCR4 inhibitor, AMD3100, and Piezo1 siRNA. Results: SMF significantly decreased the OARSI scores after induction of OA. SMF was beneficial to chondrogenesis by elevating SOX9. In the OA mouse model, an increase in MMP13 with a decrease in COL2 led to the destruction of the cartilage extracellular matrix, which was suppressed by SMF. SMF promoted the migration of cartilage-derived stem/progenitor cells and bone marrow-derived mesenchymal stem cells (MSCs). It increased SDF-1 and CXCR4, while the CXCR4 inhibitor significantly suppressed the beneficial effects of SMF. The application of Piezo1 siRNA inhibited the SMF-induced increase of CXCR4. Conclusion: SMF enhanced chondrogenesis and improved cartilage extracellular matrices. It activated the Piezo1-mediated SDF-1/CXCR4 regulatory axis and promoted the migration of endogenous stem cells. Collectively, it attenuated the pathological progression of cartilage destruction in OA mice. The Translational potential of this article: The findings in this study provided convincing evidence that SMF could enhance cartilage repair and improve OA symptoms, suggesting that SMF could have clinical value in the treatment of OA.http://www.sciencedirect.com/science/article/pii/S2214031X2200136XOsteoarthritisStatic magnetic fieldMesenchymal stem cellsChondrogenesisSDF-1/CXCR4Piezo1 |
| spellingShingle | Yuting Sun Yanwen Fang Xinle Li Jie Li Daquan Liu Min Wei Zhongcai Liao Yao Meng Lidong Zhai Hiroki Yokota Lei Yang Ying Yu Ping Zhang A static magnetic field enhances the repair of osteoarthritic cartilage by promoting the migration of stem cells and chondrogenesis Journal of Orthopaedic Translation Osteoarthritis Static magnetic field Mesenchymal stem cells Chondrogenesis SDF-1/CXCR4 Piezo1 |
| title | A static magnetic field enhances the repair of osteoarthritic cartilage by promoting the migration of stem cells and chondrogenesis |
| title_full | A static magnetic field enhances the repair of osteoarthritic cartilage by promoting the migration of stem cells and chondrogenesis |
| title_fullStr | A static magnetic field enhances the repair of osteoarthritic cartilage by promoting the migration of stem cells and chondrogenesis |
| title_full_unstemmed | A static magnetic field enhances the repair of osteoarthritic cartilage by promoting the migration of stem cells and chondrogenesis |
| title_short | A static magnetic field enhances the repair of osteoarthritic cartilage by promoting the migration of stem cells and chondrogenesis |
| title_sort | static magnetic field enhances the repair of osteoarthritic cartilage by promoting the migration of stem cells and chondrogenesis |
| topic | Osteoarthritis Static magnetic field Mesenchymal stem cells Chondrogenesis SDF-1/CXCR4 Piezo1 |
| url | http://www.sciencedirect.com/science/article/pii/S2214031X2200136X |
| work_keys_str_mv | AT yutingsun astaticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis AT yanwenfang astaticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis AT xinleli astaticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis AT jieli astaticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis AT daquanliu astaticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis AT minwei astaticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis AT zhongcailiao astaticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis AT yaomeng astaticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis AT lidongzhai astaticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis AT hirokiyokota astaticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis AT leiyang astaticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis AT yingyu astaticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis AT pingzhang astaticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis AT yutingsun staticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis AT yanwenfang staticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis AT xinleli staticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis AT jieli staticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis AT daquanliu staticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis AT minwei staticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis AT zhongcailiao staticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis AT yaomeng staticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis AT lidongzhai staticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis AT hirokiyokota staticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis AT leiyang staticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis AT yingyu staticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis AT pingzhang staticmagneticfieldenhancestherepairofosteoarthriticcartilagebypromotingthemigrationofstemcellsandchondrogenesis |