A three-layered hydrogel patch with hierarchy releasing of PLGA nanoparticle drugs decrease neointimal hyperplasia
Hydrogel is a nature scaffold that can degraded in animal body and can be used as a drug delivery system, we hypothesized that patch made of three layers of hydrogel with different PLGA nanoparticle drugs (bio-patch) can be used to decrease venous neointimal hyperplasia. Rat inferior vena cava (IVC)...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2022-01-01
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| Series: | Smart Materials in Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590183421000466 |
| _version_ | 1811173278736187392 |
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| author | Shunbo Wei Jing’an Li Hao He Chang Shu Alan Dardik Hualong Bai |
| author_facet | Shunbo Wei Jing’an Li Hao He Chang Shu Alan Dardik Hualong Bai |
| author_sort | Shunbo Wei |
| collection | DOAJ |
| description | Hydrogel is a nature scaffold that can degraded in animal body and can be used as a drug delivery system, we hypothesized that patch made of three layers of hydrogel with different PLGA nanoparticle drugs (bio-patch) can be used to decrease venous neointimal hyperplasia. Rat inferior vena cava (IVC) patch venoplasty model was used. Samples from rat IVC direct suture (DS), decellularized thoracic artery patch (TA) venoplasty and bovine pericardial patch (BPP) venoplasty were examined at day 14 after implantation. Sodium alginate and hyaluronic acid (SA/HA) hydrogel was used, three layers hydrogel patch (control) and three layers hydrogel patch with PLGA nanoparticle drugs (bio-patch) were used in rat IVC venoplasty. Patches were harvested at day 14 and analyzed. In rats, TA and BPP patch showed a thicker neointima and adventitia compared to the DS, there were larger numbers of CD68 and PCNA positive cells in both groups. The control hydrogel patch showed much thinner neointima and adventitia compared to TA and BPP patches. In both of the neointima and peri-patch area, bio-patch showed significantly fewer smooth muscle cells, fewer CD68, fewer PCNA positive cells, fewer collagen-1 positive cells, fewer p-smad2 positive cells, fewer TNF-α positive cells compared to control hydrogel patch. Bio-patch made of hydrogel and PLGA nanoparticle drugs showed a thinner neointimal thickness, and is biocompatible to the animal body. These results showed the potential application of hydrogel patch in vascular surgery. |
| first_indexed | 2024-04-10T17:45:17Z |
| format | Article |
| id | doaj.art-4d767485c7d34c9e817861fbe21a08c3 |
| institution | Directory Open Access Journal |
| issn | 2590-1834 |
| language | English |
| last_indexed | 2024-04-10T17:45:17Z |
| publishDate | 2022-01-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Smart Materials in Medicine |
| spelling | doaj.art-4d767485c7d34c9e817861fbe21a08c32023-02-03T05:01:00ZengKeAi Communications Co., Ltd.Smart Materials in Medicine2590-18342022-01-013139147A three-layered hydrogel patch with hierarchy releasing of PLGA nanoparticle drugs decrease neointimal hyperplasiaShunbo Wei0Jing’an Li1Hao He2Chang Shu3Alan Dardik4Hualong Bai5Department of Vascular and Endovascular Surgery, First Affiliated Hospital of Zhengzhou University, Henan, China; Key Vascular Physiology and Applied Research Laboratory of Zhengzhou City, Henan, ChinaSchool of Material Science and Engineering & Henan Key Laboratory of Advanced Magnesium Alloy & Key Laboratory of Materials Processing and Mold Technology (Ministry of Education), Zhengzhou University, Henan, ChinaDepartment of Vascular Surgery, the Second Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Vascular Surgery, the Second Xiangya Hospital, Central South University, Changsha, China; Department of Vascular Surgery, Fuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaThe Vascular Biology and Therapeutics Program, Yale School of Medicine, New Haven, CT, USA; Departments of Surgery and of Cellular and Molecular Physiology, Yale School of Medicine, New Haven, CT, USADepartment of Vascular and Endovascular Surgery, First Affiliated Hospital of Zhengzhou University, Henan, China; Key Vascular Physiology and Applied Research Laboratory of Zhengzhou City, Henan, China; Corresponding author. Department of Vascular and Endovascular Surgery, First Affiliated Hospital of Zhengzhou University, Longhuzhonghuan Road, Zhengdongxinqu, Zhengzhou, Henan, 450001, China.Hydrogel is a nature scaffold that can degraded in animal body and can be used as a drug delivery system, we hypothesized that patch made of three layers of hydrogel with different PLGA nanoparticle drugs (bio-patch) can be used to decrease venous neointimal hyperplasia. Rat inferior vena cava (IVC) patch venoplasty model was used. Samples from rat IVC direct suture (DS), decellularized thoracic artery patch (TA) venoplasty and bovine pericardial patch (BPP) venoplasty were examined at day 14 after implantation. Sodium alginate and hyaluronic acid (SA/HA) hydrogel was used, three layers hydrogel patch (control) and three layers hydrogel patch with PLGA nanoparticle drugs (bio-patch) were used in rat IVC venoplasty. Patches were harvested at day 14 and analyzed. In rats, TA and BPP patch showed a thicker neointima and adventitia compared to the DS, there were larger numbers of CD68 and PCNA positive cells in both groups. The control hydrogel patch showed much thinner neointima and adventitia compared to TA and BPP patches. In both of the neointima and peri-patch area, bio-patch showed significantly fewer smooth muscle cells, fewer CD68, fewer PCNA positive cells, fewer collagen-1 positive cells, fewer p-smad2 positive cells, fewer TNF-α positive cells compared to control hydrogel patch. Bio-patch made of hydrogel and PLGA nanoparticle drugs showed a thinner neointimal thickness, and is biocompatible to the animal body. These results showed the potential application of hydrogel patch in vascular surgery.http://www.sciencedirect.com/science/article/pii/S2590183421000466Three layersHydrogelVenoplastyPatchNanoparticle |
| spellingShingle | Shunbo Wei Jing’an Li Hao He Chang Shu Alan Dardik Hualong Bai A three-layered hydrogel patch with hierarchy releasing of PLGA nanoparticle drugs decrease neointimal hyperplasia Smart Materials in Medicine Three layers Hydrogel Venoplasty Patch Nanoparticle |
| title | A three-layered hydrogel patch with hierarchy releasing of PLGA nanoparticle drugs decrease neointimal hyperplasia |
| title_full | A three-layered hydrogel patch with hierarchy releasing of PLGA nanoparticle drugs decrease neointimal hyperplasia |
| title_fullStr | A three-layered hydrogel patch with hierarchy releasing of PLGA nanoparticle drugs decrease neointimal hyperplasia |
| title_full_unstemmed | A three-layered hydrogel patch with hierarchy releasing of PLGA nanoparticle drugs decrease neointimal hyperplasia |
| title_short | A three-layered hydrogel patch with hierarchy releasing of PLGA nanoparticle drugs decrease neointimal hyperplasia |
| title_sort | three layered hydrogel patch with hierarchy releasing of plga nanoparticle drugs decrease neointimal hyperplasia |
| topic | Three layers Hydrogel Venoplasty Patch Nanoparticle |
| url | http://www.sciencedirect.com/science/article/pii/S2590183421000466 |
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