Differences in Highly Pathogenic H5N6 Avian Influenza Viral Pathogenicity and Inflammatory Response in Chickens and Ducks
Infection with H5N6 highly pathogenic avian influenza virus caused high mortality in chickens, while ducks often appear to be asymptomatic. But, some recent H5Nx subtype viruses could cause high mortality in ducks. The variation between different species and the mechanisms by which some H5Nx viruses...
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Frontiers Media S.A.
2021-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.593202/full |
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| author | Bo Wang Bo Wang Qianqian Su Qianqian Su Jing Luo Meng Li Qiaoxing Wu Han Chang Han Chang Juan Du Juan Du Chengmei Huang Chengmei Huang Jiajun Ma Jiajun Ma Shuyi Han Guohui Yuan Yapeng He Minglei Guo Qingxun Zhang Hongxuan He |
| author_facet | Bo Wang Bo Wang Qianqian Su Qianqian Su Jing Luo Meng Li Qiaoxing Wu Han Chang Han Chang Juan Du Juan Du Chengmei Huang Chengmei Huang Jiajun Ma Jiajun Ma Shuyi Han Guohui Yuan Yapeng He Minglei Guo Qingxun Zhang Hongxuan He |
| author_sort | Bo Wang |
| collection | DOAJ |
| description | Infection with H5N6 highly pathogenic avian influenza virus caused high mortality in chickens, while ducks often appear to be asymptomatic. But, some recent H5Nx subtype viruses could cause high mortality in ducks. The variation between different species and the mechanisms by which some H5Nx viruses cause death in ducks requires investigation to identify the key processes in influenza susceptibility and pathogenesis. Here, we characterized two representative H5N6 viruses, A/Pavo cristatus/Jiangxi/JA1/2016 (JA1) and A/Anas crecca/shanghai/SH1/2016 (SH1), and compared their pathogenicity and expression profiles of immune-related genes in chickens and ducks to identify the elements of the host immune-related response that were involved in disease lethality. Results suggested that H5N6 HPAIVs had higher pathogenic and inflammatory effect in chickens than in ducks. Importantly, the TNF-α, IL-6, IFN-γ and iNOS levels were significantly higher in the lung of SH1 infected chickens compared to those of ducks. And we found higher systemic levels of IL-6 induced by JA1 in chickens than in ducks. In addition, our experiments demonstrated that JA1 was associated with greater pathogenicity in ducks were accompanied by the excessive expression of iNOS in the brain. These results are helpful to understand the relationship between the pathogenicity of H5N6 AIVs and inflammatory responses to them in chickens and ducks. |
| first_indexed | 2024-12-16T17:53:43Z |
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| id | doaj.art-4d76d20a7d014835922f67dedd9e4ae9 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-16T17:53:43Z |
| publishDate | 2021-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-4d76d20a7d014835922f67dedd9e4ae92022-12-21T22:22:13ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-01-011210.3389/fmicb.2021.593202593202Differences in Highly Pathogenic H5N6 Avian Influenza Viral Pathogenicity and Inflammatory Response in Chickens and DucksBo Wang0Bo Wang1Qianqian Su2Qianqian Su3Jing Luo4Meng Li5Qiaoxing Wu6Han Chang7Han Chang8Juan Du9Juan Du10Chengmei Huang11Chengmei Huang12Jiajun Ma13Jiajun Ma14Shuyi Han15Guohui Yuan16Yapeng He17Minglei Guo18Qingxun Zhang19Hongxuan He20National Research Center for Wildlife-Borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Science, University of Chinese Academy of Sciences, Beijing, ChinaNational Research Center for Wildlife-Borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Science, University of Chinese Academy of Sciences, Beijing, ChinaNational Research Center for Wildlife-Borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaNational Research Center for Wildlife-Borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaNational Research Center for Wildlife-Borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaNational Research Center for Wildlife-Borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Science, University of Chinese Academy of Sciences, Beijing, ChinaNational Research Center for Wildlife-Borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Science, University of Chinese Academy of Sciences, Beijing, ChinaNational Research Center for Wildlife-Borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Science, University of Chinese Academy of Sciences, Beijing, ChinaNational Research Center for Wildlife-Borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Science, University of Chinese Academy of Sciences, Beijing, ChinaNational Research Center for Wildlife-Borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaNational Research Center for Wildlife-Borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaNational Research Center for Wildlife-Borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaNational Research Center for Wildlife-Borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaNational Research Center for Wildlife-Borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaNational Research Center for Wildlife-Borne Diseases, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaInfection with H5N6 highly pathogenic avian influenza virus caused high mortality in chickens, while ducks often appear to be asymptomatic. But, some recent H5Nx subtype viruses could cause high mortality in ducks. The variation between different species and the mechanisms by which some H5Nx viruses cause death in ducks requires investigation to identify the key processes in influenza susceptibility and pathogenesis. Here, we characterized two representative H5N6 viruses, A/Pavo cristatus/Jiangxi/JA1/2016 (JA1) and A/Anas crecca/shanghai/SH1/2016 (SH1), and compared their pathogenicity and expression profiles of immune-related genes in chickens and ducks to identify the elements of the host immune-related response that were involved in disease lethality. Results suggested that H5N6 HPAIVs had higher pathogenic and inflammatory effect in chickens than in ducks. Importantly, the TNF-α, IL-6, IFN-γ and iNOS levels were significantly higher in the lung of SH1 infected chickens compared to those of ducks. And we found higher systemic levels of IL-6 induced by JA1 in chickens than in ducks. In addition, our experiments demonstrated that JA1 was associated with greater pathogenicity in ducks were accompanied by the excessive expression of iNOS in the brain. These results are helpful to understand the relationship between the pathogenicity of H5N6 AIVs and inflammatory responses to them in chickens and ducks.https://www.frontiersin.org/articles/10.3389/fmicb.2021.593202/fullH5N6phylogenetic analysischickens and duckspathogenic analysesinflammatory response |
| spellingShingle | Bo Wang Bo Wang Qianqian Su Qianqian Su Jing Luo Meng Li Qiaoxing Wu Han Chang Han Chang Juan Du Juan Du Chengmei Huang Chengmei Huang Jiajun Ma Jiajun Ma Shuyi Han Guohui Yuan Yapeng He Minglei Guo Qingxun Zhang Hongxuan He Differences in Highly Pathogenic H5N6 Avian Influenza Viral Pathogenicity and Inflammatory Response in Chickens and Ducks Frontiers in Microbiology H5N6 phylogenetic analysis chickens and ducks pathogenic analyses inflammatory response |
| title | Differences in Highly Pathogenic H5N6 Avian Influenza Viral Pathogenicity and Inflammatory Response in Chickens and Ducks |
| title_full | Differences in Highly Pathogenic H5N6 Avian Influenza Viral Pathogenicity and Inflammatory Response in Chickens and Ducks |
| title_fullStr | Differences in Highly Pathogenic H5N6 Avian Influenza Viral Pathogenicity and Inflammatory Response in Chickens and Ducks |
| title_full_unstemmed | Differences in Highly Pathogenic H5N6 Avian Influenza Viral Pathogenicity and Inflammatory Response in Chickens and Ducks |
| title_short | Differences in Highly Pathogenic H5N6 Avian Influenza Viral Pathogenicity and Inflammatory Response in Chickens and Ducks |
| title_sort | differences in highly pathogenic h5n6 avian influenza viral pathogenicity and inflammatory response in chickens and ducks |
| topic | H5N6 phylogenetic analysis chickens and ducks pathogenic analyses inflammatory response |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.593202/full |
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