Integrated transcriptome analysis for the hepatic and jejunal mucosa tissues of broiler chickens raised under heat stress conditions
Abstract Background Heat stress (HS) is one of the most important threats for the current poultry industry. Therefore, many efforts have been made to ameliorate the adverse effect of HS on poultry production; however, physiological and molecular mechanisms pertaining to HS are still limited in poult...
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| Format: | Article |
| Language: | English |
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BMC
2022-07-01
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| Series: | Journal of Animal Science and Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40104-022-00734-y |
| _version_ | 1811287562786963456 |
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| author | Deok Yun Kim Byeonghwi Lim Jun-Mo Kim Dong Yong Kil |
| author_facet | Deok Yun Kim Byeonghwi Lim Jun-Mo Kim Dong Yong Kil |
| author_sort | Deok Yun Kim |
| collection | DOAJ |
| description | Abstract Background Heat stress (HS) is one of the most important threats for the current poultry industry. Therefore, many efforts have been made to ameliorate the adverse effect of HS on poultry production; however, physiological and molecular mechanisms pertaining to HS are still limited in poultry. Therefore, the objective of the current study was to investigate functional alterations based on individual and integrated transcriptomes in the liver and jejunal mucosa tissues of broiler chickens exposed to HS conditions. Results Broiler chickens exposed to HS showed decreased growth performance and increased corticosterone concentrations in the feather. In the transcriptome analysis, the number of differentially expressed genes (DEGs) were identified in the liver and jejunal mucosa by HS conditions. In the liver, genes related to amino acid oxidation, tryptophan metabolism, lipid metabolism, oxidative phosphorylation, and immune responses were altered by HS, which support the reason why heat-stressed poultry had decreased productive performance. In the jejunal mucosa, genes related to defense systems, glutathione metabolism, detoxification of xenobiotics, and immune responses were differently expressed by HS conditions. The integrated transcriptome analysis with DEGs found in the liver and jejunal mucosa showed a considerable connectivity between the core nodes in the constructed networks, which includes glutathione metabolism, xenobiotic metabolism, carbon metabolism, and several amino acid metabolisms. Conclusions The core network analysis may indicate that increased requirement of energy and amino acids in the jejunal mucosa of broiler chickens exposed to HS conditions is likely compromised by increased oxidation and synthesis of amino acids in the liver. Therefore, our results may provide comprehensive insights for molecular and metabolic alterations of broiler chickens raised under HS conditions, which can aid in the development of the novel strategies to ameliorate the negative effect of HS on poultry productivity and health. |
| first_indexed | 2024-04-13T03:20:27Z |
| format | Article |
| id | doaj.art-4d78dbc469204dc49e654149d6bfa0ad |
| institution | Directory Open Access Journal |
| issn | 2049-1891 |
| language | English |
| last_indexed | 2024-04-13T03:20:27Z |
| publishDate | 2022-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Animal Science and Biotechnology |
| spelling | doaj.art-4d78dbc469204dc49e654149d6bfa0ad2022-12-22T03:04:47ZengBMCJournal of Animal Science and Biotechnology2049-18912022-07-0113111710.1186/s40104-022-00734-yIntegrated transcriptome analysis for the hepatic and jejunal mucosa tissues of broiler chickens raised under heat stress conditionsDeok Yun Kim0Byeonghwi Lim1Jun-Mo Kim2Dong Yong Kil3Department of Animal Science and Technology, Chung-Ang UniversityDepartment of Animal Science and Technology, Chung-Ang UniversityDepartment of Animal Science and Technology, Chung-Ang UniversityDepartment of Animal Science and Technology, Chung-Ang UniversityAbstract Background Heat stress (HS) is one of the most important threats for the current poultry industry. Therefore, many efforts have been made to ameliorate the adverse effect of HS on poultry production; however, physiological and molecular mechanisms pertaining to HS are still limited in poultry. Therefore, the objective of the current study was to investigate functional alterations based on individual and integrated transcriptomes in the liver and jejunal mucosa tissues of broiler chickens exposed to HS conditions. Results Broiler chickens exposed to HS showed decreased growth performance and increased corticosterone concentrations in the feather. In the transcriptome analysis, the number of differentially expressed genes (DEGs) were identified in the liver and jejunal mucosa by HS conditions. In the liver, genes related to amino acid oxidation, tryptophan metabolism, lipid metabolism, oxidative phosphorylation, and immune responses were altered by HS, which support the reason why heat-stressed poultry had decreased productive performance. In the jejunal mucosa, genes related to defense systems, glutathione metabolism, detoxification of xenobiotics, and immune responses were differently expressed by HS conditions. The integrated transcriptome analysis with DEGs found in the liver and jejunal mucosa showed a considerable connectivity between the core nodes in the constructed networks, which includes glutathione metabolism, xenobiotic metabolism, carbon metabolism, and several amino acid metabolisms. Conclusions The core network analysis may indicate that increased requirement of energy and amino acids in the jejunal mucosa of broiler chickens exposed to HS conditions is likely compromised by increased oxidation and synthesis of amino acids in the liver. Therefore, our results may provide comprehensive insights for molecular and metabolic alterations of broiler chickens raised under HS conditions, which can aid in the development of the novel strategies to ameliorate the negative effect of HS on poultry productivity and health.https://doi.org/10.1186/s40104-022-00734-yBroiler chickenHeat stressJejunal mucosaLiverTranscriptome |
| spellingShingle | Deok Yun Kim Byeonghwi Lim Jun-Mo Kim Dong Yong Kil Integrated transcriptome analysis for the hepatic and jejunal mucosa tissues of broiler chickens raised under heat stress conditions Journal of Animal Science and Biotechnology Broiler chicken Heat stress Jejunal mucosa Liver Transcriptome |
| title | Integrated transcriptome analysis for the hepatic and jejunal mucosa tissues of broiler chickens raised under heat stress conditions |
| title_full | Integrated transcriptome analysis for the hepatic and jejunal mucosa tissues of broiler chickens raised under heat stress conditions |
| title_fullStr | Integrated transcriptome analysis for the hepatic and jejunal mucosa tissues of broiler chickens raised under heat stress conditions |
| title_full_unstemmed | Integrated transcriptome analysis for the hepatic and jejunal mucosa tissues of broiler chickens raised under heat stress conditions |
| title_short | Integrated transcriptome analysis for the hepatic and jejunal mucosa tissues of broiler chickens raised under heat stress conditions |
| title_sort | integrated transcriptome analysis for the hepatic and jejunal mucosa tissues of broiler chickens raised under heat stress conditions |
| topic | Broiler chicken Heat stress Jejunal mucosa Liver Transcriptome |
| url | https://doi.org/10.1186/s40104-022-00734-y |
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