Microinjection Method for Analyzing Zebrafish Early Stage Oocytes
Maternal factors which accumulate and establish oocyte polarity during the early stages of oogenesis play key roles in embryonic development, as well as germ cell formation. However, vertebrate oogenesis, especially early stages of oogenesis, is not well understood due to the difficulty of accessing...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2021.753642/full |
| _version_ | 1818821161342992384 |
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| author | Manami Kobayashi Allison Jamieson-Lucy Mary C. Mullins |
| author_facet | Manami Kobayashi Allison Jamieson-Lucy Mary C. Mullins |
| author_sort | Manami Kobayashi |
| collection | DOAJ |
| description | Maternal factors which accumulate and establish oocyte polarity during the early stages of oogenesis play key roles in embryonic development, as well as germ cell formation. However, vertebrate oogenesis, especially early stages of oogenesis, is not well understood due to the difficulty of accessing these oocytes and the lack of analytical methods. Here, we report on a microinjection method for analyzing zebrafish early-stage oocytes and some artifacts to be aware of when performing oocyte injections or analyzing oocytes. Using this method, we successfully injected mRNAs encoding fluorescent-tagged proteins into early-stage oocytes and observed subcellular localization in the live oocytes. This method is expected to advance the functional analysis of genes involved in oogenesis. |
| first_indexed | 2024-12-18T23:03:47Z |
| format | Article |
| id | doaj.art-4d7c4bdcfcb24c4a8a1d199ca5ff22ea |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-18T23:03:47Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-4d7c4bdcfcb24c4a8a1d199ca5ff22ea2022-12-21T20:48:29ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-11-01910.3389/fcell.2021.753642753642Microinjection Method for Analyzing Zebrafish Early Stage OocytesManami KobayashiAllison Jamieson-LucyMary C. MullinsMaternal factors which accumulate and establish oocyte polarity during the early stages of oogenesis play key roles in embryonic development, as well as germ cell formation. However, vertebrate oogenesis, especially early stages of oogenesis, is not well understood due to the difficulty of accessing these oocytes and the lack of analytical methods. Here, we report on a microinjection method for analyzing zebrafish early-stage oocytes and some artifacts to be aware of when performing oocyte injections or analyzing oocytes. Using this method, we successfully injected mRNAs encoding fluorescent-tagged proteins into early-stage oocytes and observed subcellular localization in the live oocytes. This method is expected to advance the functional analysis of genes involved in oogenesis.https://www.frontiersin.org/articles/10.3389/fcell.2021.753642/fulloocytesbalbiani bodyzebrafishmicroinjectionbucky balloogenesis |
| spellingShingle | Manami Kobayashi Allison Jamieson-Lucy Mary C. Mullins Microinjection Method for Analyzing Zebrafish Early Stage Oocytes Frontiers in Cell and Developmental Biology oocytes balbiani body zebrafish microinjection bucky ball oogenesis |
| title | Microinjection Method for Analyzing Zebrafish Early Stage Oocytes |
| title_full | Microinjection Method for Analyzing Zebrafish Early Stage Oocytes |
| title_fullStr | Microinjection Method for Analyzing Zebrafish Early Stage Oocytes |
| title_full_unstemmed | Microinjection Method for Analyzing Zebrafish Early Stage Oocytes |
| title_short | Microinjection Method for Analyzing Zebrafish Early Stage Oocytes |
| title_sort | microinjection method for analyzing zebrafish early stage oocytes |
| topic | oocytes balbiani body zebrafish microinjection bucky ball oogenesis |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2021.753642/full |
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