Downregulation of lncRNA-HOXA11-AS modulates proliferation and stemness in Glioma cells
Abstract Background Glioma stem cells (GSCs) represent a subpopulation of cells within glioma that are characterized by chemotherapy resistance and tumor recurrence. GSCs are therefore important therapeutic target for glioma therapy. Long non-coding RNAs (lncRNAs) have been shown to regulate importa...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-09-01
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| Series: | Chinese Neurosurgical Journal |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s41016-017-0091-6 |
| _version_ | 1818928602759036928 |
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| author | Zuoxin Zhang Junhu Zhou Junxia Zhang Ran Duan Peiyu Pu Lei Han |
| author_facet | Zuoxin Zhang Junhu Zhou Junxia Zhang Ran Duan Peiyu Pu Lei Han |
| author_sort | Zuoxin Zhang |
| collection | DOAJ |
| description | Abstract Background Glioma stem cells (GSCs) represent a subpopulation of cells within glioma that are characterized by chemotherapy resistance and tumor recurrence. GSCs are therefore important therapeutic target for glioma therapy. Long non-coding RNAs (lncRNAs) have been shown to regulate important functions in cancer. HOXA11-AS is one such lncRNA and has been shown to regulate cell proliferation via promotion of cell cycle progression in glioblastoma (GBM) cells. However, the specific roles of HOXA11-AS in GSCs remain unclear. Methods Here we investigated the role of HOXA11-AS in driving GSC stemness properties via sphere-forming and protein chip assays. Results Gain-of-function as well as loss-of-function results showed that the HOXA11-AS maybe a critical modulator in GBM recurrence as demonstrated by cell sphere-forming ability. Furthermore, we showed that induced expression of HOXA11-AS does increase the levels of stemness-related transcription factors (Oct4/Sox17/Sox2) in U87MG cells. In vivo xenograft experiments using the HOXA11-AS knockdown U87MG cells revealed that downregulation of HOXA11-AS could strongly inhibit tumor growth. Furthermore, we found that HOXA11-AS knockdown decreased the expression of cancer stemness markers in vivo. Conclusions Collectively, these data suggests that HOXA11-AS is involved in GSC stemness and supports its clinical significance as a important therapeutic target in glioma. |
| first_indexed | 2024-12-20T03:31:31Z |
| format | Article |
| id | doaj.art-4d7dd293ce894d6ab67bb4e1389a32f1 |
| institution | Directory Open Access Journal |
| issn | 2057-4967 |
| language | English |
| last_indexed | 2024-12-20T03:31:31Z |
| publishDate | 2017-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Chinese Neurosurgical Journal |
| spelling | doaj.art-4d7dd293ce894d6ab67bb4e1389a32f12022-12-21T19:54:58ZengBMCChinese Neurosurgical Journal2057-49672017-09-01311710.1186/s41016-017-0091-6Downregulation of lncRNA-HOXA11-AS modulates proliferation and stemness in Glioma cellsZuoxin Zhang0Junhu Zhou1Junxia Zhang2Ran Duan3Peiyu Pu4Lei Han5Department of Neurosurgery, Tianjin Medical University General HospitalDepartment of Neurosurgery, Tianjin Medical University General HospitalDepartment of Neurosurgery, The First Affiliated Hospital of Nanjing Medical UniversityDepartment of Neurosurgery, Beijing Tian Tan Hospital, Capital Medical UniversityDepartment of Neurosurgery, Tianjin Medical University General HospitalDepartment of Neurosurgery, Tianjin Medical University General HospitalAbstract Background Glioma stem cells (GSCs) represent a subpopulation of cells within glioma that are characterized by chemotherapy resistance and tumor recurrence. GSCs are therefore important therapeutic target for glioma therapy. Long non-coding RNAs (lncRNAs) have been shown to regulate important functions in cancer. HOXA11-AS is one such lncRNA and has been shown to regulate cell proliferation via promotion of cell cycle progression in glioblastoma (GBM) cells. However, the specific roles of HOXA11-AS in GSCs remain unclear. Methods Here we investigated the role of HOXA11-AS in driving GSC stemness properties via sphere-forming and protein chip assays. Results Gain-of-function as well as loss-of-function results showed that the HOXA11-AS maybe a critical modulator in GBM recurrence as demonstrated by cell sphere-forming ability. Furthermore, we showed that induced expression of HOXA11-AS does increase the levels of stemness-related transcription factors (Oct4/Sox17/Sox2) in U87MG cells. In vivo xenograft experiments using the HOXA11-AS knockdown U87MG cells revealed that downregulation of HOXA11-AS could strongly inhibit tumor growth. Furthermore, we found that HOXA11-AS knockdown decreased the expression of cancer stemness markers in vivo. Conclusions Collectively, these data suggests that HOXA11-AS is involved in GSC stemness and supports its clinical significance as a important therapeutic target in glioma.http://link.springer.com/article/10.1186/s41016-017-0091-6GliomaLncRNAHOXA11-asGlioma stem cells |
| spellingShingle | Zuoxin Zhang Junhu Zhou Junxia Zhang Ran Duan Peiyu Pu Lei Han Downregulation of lncRNA-HOXA11-AS modulates proliferation and stemness in Glioma cells Chinese Neurosurgical Journal Glioma LncRNA HOXA11-as Glioma stem cells |
| title | Downregulation of lncRNA-HOXA11-AS modulates proliferation and stemness in Glioma cells |
| title_full | Downregulation of lncRNA-HOXA11-AS modulates proliferation and stemness in Glioma cells |
| title_fullStr | Downregulation of lncRNA-HOXA11-AS modulates proliferation and stemness in Glioma cells |
| title_full_unstemmed | Downregulation of lncRNA-HOXA11-AS modulates proliferation and stemness in Glioma cells |
| title_short | Downregulation of lncRNA-HOXA11-AS modulates proliferation and stemness in Glioma cells |
| title_sort | downregulation of lncrna hoxa11 as modulates proliferation and stemness in glioma cells |
| topic | Glioma LncRNA HOXA11-as Glioma stem cells |
| url | http://link.springer.com/article/10.1186/s41016-017-0091-6 |
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