The Use of High-Throughput Phenotyping for Assessment of Heat Stress-Induced Changes in Arabidopsis
The worldwide rise in heatwave frequency poses a threat to plant survival and productivity. Determining the new marker phenotypes that show reproducible response to heat stress and contribute to heat stress tolerance is becoming a priority. In this study, we describe a protocol focusing on the daily...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
American Association for the Advancement of Science (AAAS)
2020-01-01
|
| Series: | Plant Phenomics |
| Online Access: | http://dx.doi.org/10.34133/2020/3723916 |
| _version_ | 1798016816310648832 |
|---|---|
| author | Ge Gao Mark A. Tester Magdalena M. Julkowska |
| author_facet | Ge Gao Mark A. Tester Magdalena M. Julkowska |
| author_sort | Ge Gao |
| collection | DOAJ |
| description | The worldwide rise in heatwave frequency poses a threat to plant survival and productivity. Determining the new marker phenotypes that show reproducible response to heat stress and contribute to heat stress tolerance is becoming a priority. In this study, we describe a protocol focusing on the daily changes in plant morphology and photosynthetic performance after exposure to heat stress using an automated noninvasive phenotyping system. Heat stress exposure resulted in an acute reduction of the quantum yield of photosystem II and increased leaf angle. In longer term, the exposure to heat also affected plant growth and morphology. By tracking the recovery period of the WT and mutants impaired in thermotolerance (hsp101), we observed that the difference in maximum quantum yield, quenching, rosette size, and morphology. By examining the correlation across the traits throughout time, we observed that early changes in photochemical quenching corresponded with the rosette size at later stages, which suggests the contribution of quenching to overall heat tolerance. We also determined that 6 h of heat stress provides the most informative insight in plant’s responses to heat, as it shows a clear separation between treated and nontreated plants as well as the WT and hsp101. Our work streamlines future discoveries by providing an experimental protocol, data analysis pipeline, and new phenotypes that could be used as targets in thermotolerance screenings. |
| first_indexed | 2024-04-11T15:56:42Z |
| format | Article |
| id | doaj.art-4d7dfd82f4f640c1938b75232da58317 |
| institution | Directory Open Access Journal |
| issn | 2643-6515 |
| language | English |
| last_indexed | 2024-04-11T15:56:42Z |
| publishDate | 2020-01-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | Plant Phenomics |
| spelling | doaj.art-4d7dfd82f4f640c1938b75232da583172022-12-22T04:15:08ZengAmerican Association for the Advancement of Science (AAAS)Plant Phenomics2643-65152020-01-01202010.34133/2020/3723916The Use of High-Throughput Phenotyping for Assessment of Heat Stress-Induced Changes in ArabidopsisGe Gao0Mark A. Tester1Magdalena M. Julkowska2Division of Biological and Environmental Sciences and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi ArabiaDivision of Biological and Environmental Sciences and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi ArabiaDivision of Biological and Environmental Sciences and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi ArabiaThe worldwide rise in heatwave frequency poses a threat to plant survival and productivity. Determining the new marker phenotypes that show reproducible response to heat stress and contribute to heat stress tolerance is becoming a priority. In this study, we describe a protocol focusing on the daily changes in plant morphology and photosynthetic performance after exposure to heat stress using an automated noninvasive phenotyping system. Heat stress exposure resulted in an acute reduction of the quantum yield of photosystem II and increased leaf angle. In longer term, the exposure to heat also affected plant growth and morphology. By tracking the recovery period of the WT and mutants impaired in thermotolerance (hsp101), we observed that the difference in maximum quantum yield, quenching, rosette size, and morphology. By examining the correlation across the traits throughout time, we observed that early changes in photochemical quenching corresponded with the rosette size at later stages, which suggests the contribution of quenching to overall heat tolerance. We also determined that 6 h of heat stress provides the most informative insight in plant’s responses to heat, as it shows a clear separation between treated and nontreated plants as well as the WT and hsp101. Our work streamlines future discoveries by providing an experimental protocol, data analysis pipeline, and new phenotypes that could be used as targets in thermotolerance screenings.http://dx.doi.org/10.34133/2020/3723916 |
| spellingShingle | Ge Gao Mark A. Tester Magdalena M. Julkowska The Use of High-Throughput Phenotyping for Assessment of Heat Stress-Induced Changes in Arabidopsis Plant Phenomics |
| title | The Use of High-Throughput Phenotyping for Assessment of Heat Stress-Induced Changes in Arabidopsis |
| title_full | The Use of High-Throughput Phenotyping for Assessment of Heat Stress-Induced Changes in Arabidopsis |
| title_fullStr | The Use of High-Throughput Phenotyping for Assessment of Heat Stress-Induced Changes in Arabidopsis |
| title_full_unstemmed | The Use of High-Throughput Phenotyping for Assessment of Heat Stress-Induced Changes in Arabidopsis |
| title_short | The Use of High-Throughput Phenotyping for Assessment of Heat Stress-Induced Changes in Arabidopsis |
| title_sort | use of high throughput phenotyping for assessment of heat stress induced changes in arabidopsis |
| url | http://dx.doi.org/10.34133/2020/3723916 |
| work_keys_str_mv | AT gegao theuseofhighthroughputphenotypingforassessmentofheatstressinducedchangesinarabidopsis AT markatester theuseofhighthroughputphenotypingforassessmentofheatstressinducedchangesinarabidopsis AT magdalenamjulkowska theuseofhighthroughputphenotypingforassessmentofheatstressinducedchangesinarabidopsis AT gegao useofhighthroughputphenotypingforassessmentofheatstressinducedchangesinarabidopsis AT markatester useofhighthroughputphenotypingforassessmentofheatstressinducedchangesinarabidopsis AT magdalenamjulkowska useofhighthroughputphenotypingforassessmentofheatstressinducedchangesinarabidopsis |