Tiled-ClickSeq for targeted sequencing of complete coronavirus genomes with simultaneous capture of RNA recombination and minority variants

Tiled-ClickSeq for targeted sequencing of complete coronavirus genomes with simultaneous capture of RNA recombination and minority variants

High-throughput genomics of SARS-CoV-2 is essential to characterize virus evolution and to identify adaptations that affect pathogenicity or transmission. While single-nucleotide variations (SNVs) are commonly considered as driving virus adaption, RNA recombination events that delete or insert nucle...

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Bibliographic Details
Main Authors: Elizabeth Jaworski, Rose M Langsjoen, Brooke Mitchell, Barbara Judy, Patrick Newman, Jessica A Plante, Kenneth S Plante, Aaron L Miller, Yiyang Zhou, Daniele Swetnam, Stephanea Sotcheff, Victoria Morris, Nehad Saada, Rafael RG Machado, Allan McConnell, Steven G Widen, Jill Thompson, Jianli Dong, Ping Ren, Rick B Pyles, Thomas G Ksiazek, Vineet D Menachery, Scott C Weaver, Andrew L Routh
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2021-09-01
Series:eLife
Subjects:
SARS-CoV-2
ClickSeq
Genomics
Nanopore Sequencing
Defective RNAs
Next-Generation Sequencing
Online Access:https://elifesciences.org/articles/68479

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