Frequency and functional profile of circulating TCRαβ+ double negative T cells in HIV/TB co-infection
Abstract Background Increased frequency of circulating double negative T (DNT, CD4−CD8−CD3+) cells with protective immune function has been observed in human immunodeficiency virus (HIV) infection and tuberculosis (TB). Here the role of circulating TCRαβ+ DNT cells was further investigated in HIV/TB...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-11-01
|
| Series: | BMC Infectious Diseases |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12879-022-07807-3 |
| _version_ | 1811315279641182208 |
|---|---|
| author | Yuting Tan Shi Zou Wei Guo Yanni Xiang Yu Dong Qi Zhu Songjie Wu Mingqi Luo Ling Shen Ke Liang |
| author_facet | Yuting Tan Shi Zou Wei Guo Yanni Xiang Yu Dong Qi Zhu Songjie Wu Mingqi Luo Ling Shen Ke Liang |
| author_sort | Yuting Tan |
| collection | DOAJ |
| description | Abstract Background Increased frequency of circulating double negative T (DNT, CD4−CD8−CD3+) cells with protective immune function has been observed in human immunodeficiency virus (HIV) infection and tuberculosis (TB). Here the role of circulating TCRαβ+ DNT cells was further investigated in HIV/TB co-infection. Methods A cross-sectional study was conducted to investigate the frequency and functional profiles of peripheral TCRαβ+ DNT cells including apoptosis, chemokine and cytokine expression among healthy individuals and patients with TB, HIV infection and HIV/TB co-infection by cell surface staining and intracellular cytokine staining combined with flow cytometry. Results Significantly increased frequency of TCRαβ+ DNT cells was observed in HIV/TB co-infection than that in TB (p < 0.001), HIV infection (p = 0.039) and healthy controls (p < 0.001). Compared with TB, HIV/TB co-infection had higher frequency of Fas expression (p = 0.007) and lower frequency of Annexin V expression on TCRαβ+ DNT cells (p = 0.049), and the frequency of Annexin V expression on Fas+TCRαβ+ DNT cells had no significant difference. TCRαβ+ DNT cells expressed less CCR5 in HIV/TB co-infection than that in TB (p = 0.014), and more CXCR4 in HIV/TB co-infection than that in HIV infection (p = 0.043). Compared with healthy controls, TB and HIV/TB co-infection had higher frequency of TCRαβ+ DNT cells secreting Granzyme A (p = 0.046; p = 0.005). In TB and HIV/TB co-infection, TCRαβ+ DNT cells secreted more granzyme A (p = 0.002; p = 0.002) and perforin (p < 0.001; p = 0.017) than CD4+ T cells but similar to CD8+ T cells. Conclusions Reduced apoptosis may take part in the mechanism of increased frequency of peripheral TCRαβ+ DNT cells in HIV/TB co-infection. TCRαβ+ DNT cells may play a cytotoxic T cells-like function in HIV/TB co-infection. |
| first_indexed | 2024-04-13T11:28:33Z |
| format | Article |
| id | doaj.art-4d7f2efd153b4899a4ca29f07e9e70de |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-04-13T11:28:33Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-4d7f2efd153b4899a4ca29f07e9e70de2022-12-22T02:48:38ZengBMCBMC Infectious Diseases1471-23342022-11-012211910.1186/s12879-022-07807-3Frequency and functional profile of circulating TCRαβ+ double negative T cells in HIV/TB co-infectionYuting Tan0Shi Zou1Wei Guo2Yanni Xiang3Yu Dong4Qi Zhu5Songjie Wu6Mingqi Luo7Ling Shen8Ke Liang9Department of Infectious Diseases, Zhongnan Hospital of Wuhan UniversityDepartment of Infectious Diseases, Zhongnan Hospital of Wuhan UniversityWuhan Research Center for Infectious Diseases and Cancer, Chinese Academy of Medical SciencesDepartment of Intensive Care Medicine, Yichang Central People’s HospitalDepartment of Geriatrics, Zhongnan Hospital of Wuhan UniversityWuhan Pulmonary Hospital, Wuhan Institute for Tuberculosis ControlWuhan Research Center for Infectious Diseases and Cancer, Chinese Academy of Medical SciencesDepartment of Infectious Diseases, Zhongnan Hospital of Wuhan UniversityDepartment of Microbiology and Immunology, Center for Primate Biomedical Research, University of Illinois College of MedicineDepartment of Infectious Diseases, Zhongnan Hospital of Wuhan UniversityAbstract Background Increased frequency of circulating double negative T (DNT, CD4−CD8−CD3+) cells with protective immune function has been observed in human immunodeficiency virus (HIV) infection and tuberculosis (TB). Here the role of circulating TCRαβ+ DNT cells was further investigated in HIV/TB co-infection. Methods A cross-sectional study was conducted to investigate the frequency and functional profiles of peripheral TCRαβ+ DNT cells including apoptosis, chemokine and cytokine expression among healthy individuals and patients with TB, HIV infection and HIV/TB co-infection by cell surface staining and intracellular cytokine staining combined with flow cytometry. Results Significantly increased frequency of TCRαβ+ DNT cells was observed in HIV/TB co-infection than that in TB (p < 0.001), HIV infection (p = 0.039) and healthy controls (p < 0.001). Compared with TB, HIV/TB co-infection had higher frequency of Fas expression (p = 0.007) and lower frequency of Annexin V expression on TCRαβ+ DNT cells (p = 0.049), and the frequency of Annexin V expression on Fas+TCRαβ+ DNT cells had no significant difference. TCRαβ+ DNT cells expressed less CCR5 in HIV/TB co-infection than that in TB (p = 0.014), and more CXCR4 in HIV/TB co-infection than that in HIV infection (p = 0.043). Compared with healthy controls, TB and HIV/TB co-infection had higher frequency of TCRαβ+ DNT cells secreting Granzyme A (p = 0.046; p = 0.005). In TB and HIV/TB co-infection, TCRαβ+ DNT cells secreted more granzyme A (p = 0.002; p = 0.002) and perforin (p < 0.001; p = 0.017) than CD4+ T cells but similar to CD8+ T cells. Conclusions Reduced apoptosis may take part in the mechanism of increased frequency of peripheral TCRαβ+ DNT cells in HIV/TB co-infection. TCRαβ+ DNT cells may play a cytotoxic T cells-like function in HIV/TB co-infection.https://doi.org/10.1186/s12879-022-07807-3Human immunodeficiency virusTuberculosisTCRαβ+ double negative T cellsFas, CCR5Granzyme A |
| spellingShingle | Yuting Tan Shi Zou Wei Guo Yanni Xiang Yu Dong Qi Zhu Songjie Wu Mingqi Luo Ling Shen Ke Liang Frequency and functional profile of circulating TCRαβ+ double negative T cells in HIV/TB co-infection BMC Infectious Diseases Human immunodeficiency virus Tuberculosis TCRαβ+ double negative T cells Fas, CCR5 Granzyme A |
| title | Frequency and functional profile of circulating TCRαβ+ double negative T cells in HIV/TB co-infection |
| title_full | Frequency and functional profile of circulating TCRαβ+ double negative T cells in HIV/TB co-infection |
| title_fullStr | Frequency and functional profile of circulating TCRαβ+ double negative T cells in HIV/TB co-infection |
| title_full_unstemmed | Frequency and functional profile of circulating TCRαβ+ double negative T cells in HIV/TB co-infection |
| title_short | Frequency and functional profile of circulating TCRαβ+ double negative T cells in HIV/TB co-infection |
| title_sort | frequency and functional profile of circulating tcrαβ double negative t cells in hiv tb co infection |
| topic | Human immunodeficiency virus Tuberculosis TCRαβ+ double negative T cells Fas, CCR5 Granzyme A |
| url | https://doi.org/10.1186/s12879-022-07807-3 |
| work_keys_str_mv | AT yutingtan frequencyandfunctionalprofileofcirculatingtcrabdoublenegativetcellsinhivtbcoinfection AT shizou frequencyandfunctionalprofileofcirculatingtcrabdoublenegativetcellsinhivtbcoinfection AT weiguo frequencyandfunctionalprofileofcirculatingtcrabdoublenegativetcellsinhivtbcoinfection AT yannixiang frequencyandfunctionalprofileofcirculatingtcrabdoublenegativetcellsinhivtbcoinfection AT yudong frequencyandfunctionalprofileofcirculatingtcrabdoublenegativetcellsinhivtbcoinfection AT qizhu frequencyandfunctionalprofileofcirculatingtcrabdoublenegativetcellsinhivtbcoinfection AT songjiewu frequencyandfunctionalprofileofcirculatingtcrabdoublenegativetcellsinhivtbcoinfection AT mingqiluo frequencyandfunctionalprofileofcirculatingtcrabdoublenegativetcellsinhivtbcoinfection AT lingshen frequencyandfunctionalprofileofcirculatingtcrabdoublenegativetcellsinhivtbcoinfection AT keliang frequencyandfunctionalprofileofcirculatingtcrabdoublenegativetcellsinhivtbcoinfection |