Association of rs9679162 Genetic Polymorphism and Aberrant Expression of Polypeptide <i>N-Acetylgalactosaminyltransferase 14 (GALNT14)</i> in Head and Neck Cancer
The polypeptide <i>N-Acetylgalactosaminyltransferase 14 (GALNT14)</i> rs9679162 and mRNA expression were associated with treatment outcome in various cancers. However, the relation of <i>GALNT14</i> and head and neck cancer were nuclear. A total of 199 patients with head and...
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MDPI AG
2022-08-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/14/17/4217 |
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| author | Nan-Chin Lin Yin-Hwa Shih Kuo-Chou Chiu Po-Jung Li Hui-Wu Yang Wan-Chen Lan Shih-Min Hsia Tong-Hong Wang Tzong-Ming Shieh |
| author_facet | Nan-Chin Lin Yin-Hwa Shih Kuo-Chou Chiu Po-Jung Li Hui-Wu Yang Wan-Chen Lan Shih-Min Hsia Tong-Hong Wang Tzong-Ming Shieh |
| author_sort | Nan-Chin Lin |
| collection | DOAJ |
| description | The polypeptide <i>N-Acetylgalactosaminyltransferase 14 (GALNT14)</i> rs9679162 and mRNA expression were associated with treatment outcome in various cancers. However, the relation of <i>GALNT14</i> and head and neck cancer were nuclear. A total of 199 patients with head and neck squamous cell carcinoma (HNSCC) were collected in this study, including oral SCC (OSCC), oropharyngeal SCC (OPSCC), laryngeal SCC (LSCC), and others. The DNA and RNA of cancer tissues were extracted using the TRI Reagent method. The rs9679162 was analyzed using polymerase chain reaction (PCR) and sequencing methods in 199 DNA specimens, and the mRNA expression was analyzed using quantitative reverse transcription PCR (RT-qPCR) methods in 68 paired RNA specimens of non-cancerous matched tissues (NCMT) and tumor tissues. The results showed that the genotype of TT, TG, and GG appeared at 30%, 44%, and 26%, respectively. Non-TT genotype or G alleotype were associated with alcohol, betel nut, and cigarette using among patients with OSCC, and it also affected the treatment and survival of patients with OSCC and LSCC. High <i>GALNT14</i> mRNA expression levels increased lymphatic metastasis of patients with HNSCC, and treatment and survival in patients with OPSCC. Overall, the <i>GALNT14</i>-rs9679162 genotype and mRNA expression level can be used as indicators of HNSCC treatment prognosis. |
| first_indexed | 2024-03-10T01:58:45Z |
| format | Article |
| id | doaj.art-4d7f3b23602c4f2ba8597045e9526c06 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T01:58:45Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-4d7f3b23602c4f2ba8597045e9526c062023-11-23T12:51:47ZengMDPI AGCancers2072-66942022-08-011417421710.3390/cancers14174217Association of rs9679162 Genetic Polymorphism and Aberrant Expression of Polypeptide <i>N-Acetylgalactosaminyltransferase 14 (GALNT14)</i> in Head and Neck CancerNan-Chin Lin0Yin-Hwa Shih1Kuo-Chou Chiu2Po-Jung Li3Hui-Wu Yang4Wan-Chen Lan5Shih-Min Hsia6Tong-Hong Wang7Tzong-Ming Shieh8Department of Oral and Maxillofacial Surgery, Show Chwan Memorial Hospital, Changhua 500009, TaiwanDepartment of Healthcare Administration, Asia University, Taichung 41354, TaiwanDivision of Oral Diagnosis and Family Dentistry, Tri-Service General Hospital, National Defense Medical Center, Taipei 11490, TaiwanSchool of Dentistry, China Medical University, Taichung 40402, TaiwanSchool of Dentistry, China Medical University, Taichung 40402, TaiwanDepartment of Healthcare Administration, Asia University, Taichung 41354, TaiwanSchool of Nutrition and Health Sciences, Taipei Medical University, Taipei 110301, TaiwanGraduate Institute of Health Industry Technology and Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan 33305, TaiwanSchool of Dentistry, China Medical University, Taichung 40402, TaiwanThe polypeptide <i>N-Acetylgalactosaminyltransferase 14 (GALNT14)</i> rs9679162 and mRNA expression were associated with treatment outcome in various cancers. However, the relation of <i>GALNT14</i> and head and neck cancer were nuclear. A total of 199 patients with head and neck squamous cell carcinoma (HNSCC) were collected in this study, including oral SCC (OSCC), oropharyngeal SCC (OPSCC), laryngeal SCC (LSCC), and others. The DNA and RNA of cancer tissues were extracted using the TRI Reagent method. The rs9679162 was analyzed using polymerase chain reaction (PCR) and sequencing methods in 199 DNA specimens, and the mRNA expression was analyzed using quantitative reverse transcription PCR (RT-qPCR) methods in 68 paired RNA specimens of non-cancerous matched tissues (NCMT) and tumor tissues. The results showed that the genotype of TT, TG, and GG appeared at 30%, 44%, and 26%, respectively. Non-TT genotype or G alleotype were associated with alcohol, betel nut, and cigarette using among patients with OSCC, and it also affected the treatment and survival of patients with OSCC and LSCC. High <i>GALNT14</i> mRNA expression levels increased lymphatic metastasis of patients with HNSCC, and treatment and survival in patients with OPSCC. Overall, the <i>GALNT14</i>-rs9679162 genotype and mRNA expression level can be used as indicators of HNSCC treatment prognosis.https://www.mdpi.com/2072-6694/14/17/4217alcoholbetel nut<i>GALNT14</i>head and neck cancerrs9679162survival |
| spellingShingle | Nan-Chin Lin Yin-Hwa Shih Kuo-Chou Chiu Po-Jung Li Hui-Wu Yang Wan-Chen Lan Shih-Min Hsia Tong-Hong Wang Tzong-Ming Shieh Association of rs9679162 Genetic Polymorphism and Aberrant Expression of Polypeptide <i>N-Acetylgalactosaminyltransferase 14 (GALNT14)</i> in Head and Neck Cancer Cancers alcohol betel nut <i>GALNT14</i> head and neck cancer rs9679162 survival |
| title | Association of rs9679162 Genetic Polymorphism and Aberrant Expression of Polypeptide <i>N-Acetylgalactosaminyltransferase 14 (GALNT14)</i> in Head and Neck Cancer |
| title_full | Association of rs9679162 Genetic Polymorphism and Aberrant Expression of Polypeptide <i>N-Acetylgalactosaminyltransferase 14 (GALNT14)</i> in Head and Neck Cancer |
| title_fullStr | Association of rs9679162 Genetic Polymorphism and Aberrant Expression of Polypeptide <i>N-Acetylgalactosaminyltransferase 14 (GALNT14)</i> in Head and Neck Cancer |
| title_full_unstemmed | Association of rs9679162 Genetic Polymorphism and Aberrant Expression of Polypeptide <i>N-Acetylgalactosaminyltransferase 14 (GALNT14)</i> in Head and Neck Cancer |
| title_short | Association of rs9679162 Genetic Polymorphism and Aberrant Expression of Polypeptide <i>N-Acetylgalactosaminyltransferase 14 (GALNT14)</i> in Head and Neck Cancer |
| title_sort | association of rs9679162 genetic polymorphism and aberrant expression of polypeptide i n acetylgalactosaminyltransferase 14 galnt14 i in head and neck cancer |
| topic | alcohol betel nut <i>GALNT14</i> head and neck cancer rs9679162 survival |
| url | https://www.mdpi.com/2072-6694/14/17/4217 |
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