Gestational Day-Dependent Expression of Interleukin-10 and Tumor Necrosis Factor-alpha in Porphyromonas gingivalis-infected Pregnant Rats
<!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:TrackMoves /> <w:TrackFormatting /> <w:DoNotShowRevisions /> <w:DoNotPrintRevisions /> <w:DoNotShowMarkup /> <w:DoNotShowComments /> &...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Faculty of Dentistry, Universitas Indonesia
2014-04-01
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| Series: | Journal of Dentistry Indonesia |
| Subjects: | |
| Online Access: | http://jdentistry.ui.ac.id/index.php/JDI/article/view/199 |
| Summary: | <!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:TrackMoves /> <w:TrackFormatting /> <w:DoNotShowRevisions /> <w:DoNotPrintRevisions /> <w:DoNotShowMarkup /> <w:DoNotShowComments /> <w:DoNotShowInsertionsAndDeletions /> <w:DoNotShowPropertyChanges /> <w:PunctuationKerning /> <w:ValidateAgainstSchemas /> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:DoNotPromoteQF /> <w:LidThemeOther>IN</w:LidThemeOther> <w:LidThemeAsian>X-NONE</w:LidThemeAsian> <w:LidThemeComplexScript>X-NONE</w:LidThemeComplexScript> <w:Compatibility> <w:BreakWrappedTables /> <w:SnapToGridInCell /> <w:WrapTextWithPunct /> <w:UseAsianBreakRules /> <w:DontGrowAutofit /> <w:SplitPgBreakAndParaMark /> <w:DontVertAlignCellWithSp /> <w:DontBreakConstrainedForcedTables /> <w:DontVertAlignInTxbx /> <w:Word11KerningPairs /> <w:CachedColBalance /> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> <m:mathPr> <m:mathFont m:val="Cambria Math" /> <m:brkBin m:val="before" /> <m:brkBinSub m:val=" " /> <m:smallFrac m:val="off" /> <m:dispDef /> <m:lMargin m:val="0" /> <m:rMargin m:val="0" /> <m:defJc m:val="centerGroup" /> <m:wrapIndent m:val="1440" /> <m:intLim m:val="subSup" /> <m:naryLim m:val="undOvr" /> </m:mathPr></w:WordDocument> </xml><![endif]--> <p class="MsoNormal" style="text-align: left; line-height: normal; mso-layout-grid-align: none; text-autospace: none;"><span style="font-size: 8.0pt; mso-bidi-font-size: 10.0pt; font-family: "TimesNewRomanPSMT","serif"; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: IN;">Fetal growth restriction remains a major cause of neonatal morbidity and mortality. </span><em><span style="font-size: 8.0pt; mso-bidi-font-size: 10.0pt; font-family: "TimesNewRomanPS-ItalicMT","serif"; mso-bidi-font-family: TimesNewRomanPS-ItalicMT; mso-ansi-language: IN;">Porphyromonas gingivalis</span></em><span style="font-size: 8.0pt; mso-bidi-font-size: 10.0pt; font-family: "TimesNewRomanPSMT","serif"; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: IN;">can induce placental inflammatory response resulting in fetal growth restriction. </span><strong><span style="font-size: 8.0pt; mso-bidi-font-size: 10.0pt; font-family: "TimesNewRomanPS-BoldMT","serif"; mso-bidi-font-family: TimesNewRomanPS-BoldMT; mso-ansi-language: IN;">Objective: </span></strong><span style="font-size: 8.0pt; mso-bidi-font-size: 10.0pt; font-family: "TimesNewRomanPSMT","serif"; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: IN;">This study aimed to evaluate the potential utility of the pro-inflammatory cytokine TNF-α and anti-inflammatory cytokine IL-10 in rat placental tissues to understand whether these events were causally related. </span><strong><span style="font-size: 8.0pt; mso-bidi-font-size: 10.0pt; font-family: "TimesNewRomanPS-BoldMT","serif"; mso-bidi-font-family: TimesNewRomanPS-BoldMT; mso-ansi-language: IN;">Methods: </span></strong><span style="font-size: 8.0pt; mso-bidi-font-size: 10.0pt; font-family: "TimesNewRomanPSMT","serif"; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: IN;">Female rats were infected with live-</span><em><span style="font-size: 8.0pt; mso-bidi-font-size: 10.0pt; font-family: "TimesNewRomanPS-ItalicMT","serif"; mso-bidi-font-family: TimesNewRomanPS-ItalicMT; mso-ansi-language: IN;">Porphyromonas gingivalis </span></em><span style="font-size: 8.0pt; mso-bidi-font-size: 10.0pt; font-family: "TimesNewRomanPSMT","serif"; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: IN;">at concentration of 2x10</span><span style="font-size: 8.0pt; mso-bidi-font-size: 6.0pt; font-family: "TimesNewRomanPSMT","serif"; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: IN;">9 </span><span style="font-size: 8.0pt; mso-bidi-font-size: 10.0pt; font-family: "TimesNewRomanPSMT","serif"; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: IN;">cells/ml into subgingival sulcus area of the maxillary first molar before and/or during pregnancy. They were sacrificed on gestational day (GD)-14 and GD20. The expression of TNF-α and IL-10 in macrophages and trophoblast cells were detected by <span style="mso-spacerun: yes;"> </span>immunohistochemistry. </span><strong><span style="font-size: 8.0pt; mso-bidi-font-size: 10.0pt; font-family: "TimesNewRomanPS-BoldMT","serif"; mso-bidi-font-family: TimesNewRomanPS-BoldMT; mso-ansi-language: IN;">Results: </span></strong><span style="font-size: 8.0pt; mso-bidi-font-size: 10.0pt; font-family: "TimesNewRomanPSMT","serif"; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: IN;">A higher expression of TNF-α was found in spongiotrophoblast of the Pg-BD group on GD14 (6.30±1.16), and in trophoblastic giant cells of Pg-D group on GD20 (5.50±1.35). Furthermore, a higher expression of IL-10 was found in trophoblastic giant cells of the Pg-BD group on GD14 (4.50±1.51) and in syncytiotrophoblasts of Pg-BD group on GD20 (8.70±2.67). </span><strong><span style="font-size: 8.0pt; mso-bidi-font-size: 10.0pt; font-family: "TimesNewRomanPS-BoldMT","serif"; mso-bidi-font-family: TimesNewRomanPS-BoldMT; mso-ansi-language: IN;">Conclusion: </span></strong><span style="font-size: 8.0pt; mso-bidi-font-size: 10.0pt; font-family: "TimesNewRomanPSMT","serif"; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: IN;">The expression of TNF-α on GD14 and GD20 were accompanied by increased expression of IL-10. The placental pathologic conditions induced by </span><em><span style="font-size: 8.0pt; mso-bidi-font-size: 10.0pt; font-family: "TimesNewRomanPS-ItalicMT","serif"; mso-bidi-font-family: TimesNewRomanPS-ItalicMT; mso-ansi-language: IN;">Porphyromonas gingivalis </span></em><span style="font-size: 8.0pt; mso-bidi-font-size: 10.0pt; font-family: "TimesNewRomanPSMT","serif"; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: IN;">can be inhibited by elevated expression of IL-10 in macrophages and trophoblast cells.</span></p><p class="MsoNormal" style="text-align: left; line-height: normal; mso-layout-grid-align: none; text-autospace: none;"><span style="font-size: 8.0pt; mso-bidi-font-size: 10.0pt; font-family: "TimesNewRomanPSMT","serif"; mso-bidi-font-family: TimesNewRomanPSMT; mso-ansi-language: IN;">DOI: </span>10.14693/jdi.v20i3.199</p> |
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| ISSN: | 1693-9697 2355-4800 |