Antibacterial activity of bioactive compound produced by endophytic fungi isolated from Mangifera casturi Kosterm endemic plant from South Kalimantan, Indonesia
The endophytic fungi that live in endemic plants are a promising bio‐prospect as the producers of antibacterial compounds. This research is aimed to evaluate the endophytic fungi antibacterial compound from Mangifera casturi. The bioactive compounds of 13 endophytic fungi were extracted using ethyl...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Gadjah Mada, Yogyakarta
2023-06-01
|
| Series: | Indonesian Journal of Biotechnology |
| Subjects: | |
| Online Access: | https://jurnal.ugm.ac.id/ijbiotech/article/view/71150 |
| _version_ | 1827907610320306176 |
|---|---|
| author | Fatia Rizki Nuraini Ratna Setyaningsih Ari Susilowati |
| author_facet | Fatia Rizki Nuraini Ratna Setyaningsih Ari Susilowati |
| author_sort | Fatia Rizki Nuraini |
| collection | DOAJ |
| description | The endophytic fungi that live in endemic plants are a promising bio‐prospect as the producers of antibacterial compounds. This research is aimed to evaluate the endophytic fungi antibacterial compound from Mangifera casturi. The bioactive compounds of 13 endophytic fungi were extracted using ethyl acetate and evaluated for antibacterial activity using disk diffusion assay. The minimum inhibitory concentration (MIC) was measured using the serial broth dilution method. Scanning Electron Microscopy (SEM) was used to examine cell damage because of the extract. The antibacterial compounds were then detected using GC‐MS analysis. The endophytic fungi were identified morphologically and molecularly based on ITS rDNA sequencing Among the 13 isolates, the endophytic fungi identified as Botryosphaeria rhodina AK32 produced the antibacterial compounds that exhibited the highest activity and a broad spectrum. Moreover, they were capable against resistant bacteria, Methicillin Resistant Staphylococcus aureus (MRSA) with an MIC value of 1.56% for all the test bacteria. The mechanism of action of AK32 ethyl acetate extract seemed to affect the condition of bacterial cell walls, causing morphological alteration such as shrinkage of the cell, warted cells, and hollow cells. Based on GC‐MS, the antibacterial compounds of AK32 ethyl acetate extract were di‐n‐octyl phthalate, benzyl alcohol, high‐oleic (CAS) safflower oil, benzene acetonitrile, and benzotriazole. |
| first_indexed | 2024-03-13T01:10:34Z |
| format | Article |
| id | doaj.art-4d805554b6cf42e18c2303293597807f |
| institution | Directory Open Access Journal |
| issn | 0853-8654 2089-2241 |
| language | English |
| last_indexed | 2024-03-13T01:10:34Z |
| publishDate | 2023-06-01 |
| publisher | Universitas Gadjah Mada, Yogyakarta |
| record_format | Article |
| series | Indonesian Journal of Biotechnology |
| spelling | doaj.art-4d805554b6cf42e18c2303293597807f2023-07-06T03:13:33ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412023-06-01282778510.22146/ijbiotech.7115034020Antibacterial activity of bioactive compound produced by endophytic fungi isolated from Mangifera casturi Kosterm endemic plant from South Kalimantan, IndonesiaFatia Rizki Nuraini0Ratna Setyaningsih1Ari Susilowati2Department of Blood Bank Technology, Undergraduate Program, Stikes Rajekwesi Bojonegoro Jalan Raya Dander, Ngumpak Dalem, Dander, Bojonegoro, East Java 62171, IndonesiaDepartment of Biology, Graduate Program, Universitas Sebelas Maret, Jl. Ir. Sutami No. 36A, Kentingan, Surakarta, Central Java 57126, IndonesiaDepartment of Biology, Graduate Program, Universitas Sebelas Maret, Jl. Ir. Sutami No. 36A, Kentingan, Surakarta, Central Java 57126, IndonesiaThe endophytic fungi that live in endemic plants are a promising bio‐prospect as the producers of antibacterial compounds. This research is aimed to evaluate the endophytic fungi antibacterial compound from Mangifera casturi. The bioactive compounds of 13 endophytic fungi were extracted using ethyl acetate and evaluated for antibacterial activity using disk diffusion assay. The minimum inhibitory concentration (MIC) was measured using the serial broth dilution method. Scanning Electron Microscopy (SEM) was used to examine cell damage because of the extract. The antibacterial compounds were then detected using GC‐MS analysis. The endophytic fungi were identified morphologically and molecularly based on ITS rDNA sequencing Among the 13 isolates, the endophytic fungi identified as Botryosphaeria rhodina AK32 produced the antibacterial compounds that exhibited the highest activity and a broad spectrum. Moreover, they were capable against resistant bacteria, Methicillin Resistant Staphylococcus aureus (MRSA) with an MIC value of 1.56% for all the test bacteria. The mechanism of action of AK32 ethyl acetate extract seemed to affect the condition of bacterial cell walls, causing morphological alteration such as shrinkage of the cell, warted cells, and hollow cells. Based on GC‐MS, the antibacterial compounds of AK32 ethyl acetate extract were di‐n‐octyl phthalate, benzyl alcohol, high‐oleic (CAS) safflower oil, benzene acetonitrile, and benzotriazole.https://jurnal.ugm.ac.id/ijbiotech/article/view/71150antibacterial activity, botryosphaeria rhodina, endophytic fungi, mangifera casturi, mrsa |
| spellingShingle | Fatia Rizki Nuraini Ratna Setyaningsih Ari Susilowati Antibacterial activity of bioactive compound produced by endophytic fungi isolated from Mangifera casturi Kosterm endemic plant from South Kalimantan, Indonesia Indonesian Journal of Biotechnology antibacterial activity, botryosphaeria rhodina, endophytic fungi, mangifera casturi, mrsa |
| title | Antibacterial activity of bioactive compound produced by endophytic fungi isolated from Mangifera casturi Kosterm endemic plant from South Kalimantan, Indonesia |
| title_full | Antibacterial activity of bioactive compound produced by endophytic fungi isolated from Mangifera casturi Kosterm endemic plant from South Kalimantan, Indonesia |
| title_fullStr | Antibacterial activity of bioactive compound produced by endophytic fungi isolated from Mangifera casturi Kosterm endemic plant from South Kalimantan, Indonesia |
| title_full_unstemmed | Antibacterial activity of bioactive compound produced by endophytic fungi isolated from Mangifera casturi Kosterm endemic plant from South Kalimantan, Indonesia |
| title_short | Antibacterial activity of bioactive compound produced by endophytic fungi isolated from Mangifera casturi Kosterm endemic plant from South Kalimantan, Indonesia |
| title_sort | antibacterial activity of bioactive compound produced by endophytic fungi isolated from mangifera casturi kosterm endemic plant from south kalimantan indonesia |
| topic | antibacterial activity, botryosphaeria rhodina, endophytic fungi, mangifera casturi, mrsa |
| url | https://jurnal.ugm.ac.id/ijbiotech/article/view/71150 |
| work_keys_str_mv | AT fatiarizkinuraini antibacterialactivityofbioactivecompoundproducedbyendophyticfungiisolatedfrommangiferacasturikostermendemicplantfromsouthkalimantanindonesia AT ratnasetyaningsih antibacterialactivityofbioactivecompoundproducedbyendophyticfungiisolatedfrommangiferacasturikostermendemicplantfromsouthkalimantanindonesia AT arisusilowati antibacterialactivityofbioactivecompoundproducedbyendophyticfungiisolatedfrommangiferacasturikostermendemicplantfromsouthkalimantanindonesia |