Chitosan-Based Nanogels: Synthesis and Toxicity Profile for Drug Delivery to Articular Joints
One important challenge in treating avascular-degraded cartilage is the development of new drugs for both pain management and joint preservation. Considerable efforts have been invested in developing nanosystems using biomaterials, such as chitosan, a widely used natural polymer exhibiting numerous...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/8/1337 |
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| author | Seng Manivong Araceli Garcia Ac Shunmoogum A. Patten Julio C. Fernandes Mohamed Benderdour Xavier Banquy Florina Moldovan Valérie Gaëlle Roullin |
| author_facet | Seng Manivong Araceli Garcia Ac Shunmoogum A. Patten Julio C. Fernandes Mohamed Benderdour Xavier Banquy Florina Moldovan Valérie Gaëlle Roullin |
| author_sort | Seng Manivong |
| collection | DOAJ |
| description | One important challenge in treating avascular-degraded cartilage is the development of new drugs for both pain management and joint preservation. Considerable efforts have been invested in developing nanosystems using biomaterials, such as chitosan, a widely used natural polymer exhibiting numerous advantages, i.e., non-toxic, biocompatible and biodegradable. However, even if chitosan is generally recognized as safe, the safety and biocompatibility of such nanomaterials must be addressed because of potential for greater interactions between nanomaterials and biological systems. Here, we developed chitosan-based nanogels as drug-delivery platforms and established an initial biological risk assessment for osteocartilaginous applications. We investigated the influence of synthesis parameters on the physicochemical characteristics of the resulting nanogels and their potential impact on the biocompatibility on all types of human osteocartilaginous cells. Monodisperse nanogels were synthesized with sizes ranging from 268 to 382 nm according to the acidic solution used (i.e., either citric or acetic acid) with overall positive charge surface. Our results demonstrated that purified chitosan-based nanogels neither affected cell proliferation nor induced nitric oxide production in vitro. However, nanogels were moderately genotoxic in a dose-dependent manner but did not significantly induce acute embryotoxicity in zebrafish embryos, up to 100 µg∙mL<sup>−1</sup>. These encouraging results hold great promise for the intra-articular delivery of drugs or diagnostic agents for joint pathologies. |
| first_indexed | 2024-03-09T13:12:28Z |
| format | Article |
| id | doaj.art-4d84442f006e47c48bcd84c8255f1ad9 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T13:12:28Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-4d84442f006e47c48bcd84c8255f1ad92023-11-30T21:40:03ZengMDPI AGNanomaterials2079-49912022-04-01128133710.3390/nano12081337Chitosan-Based Nanogels: Synthesis and Toxicity Profile for Drug Delivery to Articular JointsSeng Manivong0Araceli Garcia Ac1Shunmoogum A. Patten2Julio C. Fernandes3Mohamed Benderdour4Xavier Banquy5Florina Moldovan6Valérie Gaëlle Roullin7Centre de Recherche, CHU Sainte-Justine, Montreal, QC H3T 1C5, CanadaFaculté de Pharmacie, Université de Montréal, Montreal, QC H3T 1J4, CanadaInstitut National de la Recherche Scientifique, Centre Armand-Frappier Santé Biotechnologie, Laval, QC H7V 1B7, CanadaCentre de Recherche, Hôpital du Sacré-Cœur de Montréal, Faculté de Médecine, Université de Montréal, Montreal, QC H4J 1C5, CanadaCentre de Recherche, Hôpital du Sacré-Cœur de Montréal, Faculté de Médecine, Université de Montréal, Montreal, QC H4J 1C5, CanadaFaculté de Pharmacie, Université de Montréal, Montreal, QC H3T 1J4, CanadaCentre de Recherche, CHU Sainte-Justine, Montreal, QC H3T 1C5, CanadaFaculté de Pharmacie, Université de Montréal, Montreal, QC H3T 1J4, CanadaOne important challenge in treating avascular-degraded cartilage is the development of new drugs for both pain management and joint preservation. Considerable efforts have been invested in developing nanosystems using biomaterials, such as chitosan, a widely used natural polymer exhibiting numerous advantages, i.e., non-toxic, biocompatible and biodegradable. However, even if chitosan is generally recognized as safe, the safety and biocompatibility of such nanomaterials must be addressed because of potential for greater interactions between nanomaterials and biological systems. Here, we developed chitosan-based nanogels as drug-delivery platforms and established an initial biological risk assessment for osteocartilaginous applications. We investigated the influence of synthesis parameters on the physicochemical characteristics of the resulting nanogels and their potential impact on the biocompatibility on all types of human osteocartilaginous cells. Monodisperse nanogels were synthesized with sizes ranging from 268 to 382 nm according to the acidic solution used (i.e., either citric or acetic acid) with overall positive charge surface. Our results demonstrated that purified chitosan-based nanogels neither affected cell proliferation nor induced nitric oxide production in vitro. However, nanogels were moderately genotoxic in a dose-dependent manner but did not significantly induce acute embryotoxicity in zebrafish embryos, up to 100 µg∙mL<sup>−1</sup>. These encouraging results hold great promise for the intra-articular delivery of drugs or diagnostic agents for joint pathologies.https://www.mdpi.com/2079-4991/12/8/1337nanogelbiopolymerhyaluronic acidosteoarthritisbiocompatibilitycartilage |
| spellingShingle | Seng Manivong Araceli Garcia Ac Shunmoogum A. Patten Julio C. Fernandes Mohamed Benderdour Xavier Banquy Florina Moldovan Valérie Gaëlle Roullin Chitosan-Based Nanogels: Synthesis and Toxicity Profile for Drug Delivery to Articular Joints Nanomaterials nanogel biopolymer hyaluronic acid osteoarthritis biocompatibility cartilage |
| title | Chitosan-Based Nanogels: Synthesis and Toxicity Profile for Drug Delivery to Articular Joints |
| title_full | Chitosan-Based Nanogels: Synthesis and Toxicity Profile for Drug Delivery to Articular Joints |
| title_fullStr | Chitosan-Based Nanogels: Synthesis and Toxicity Profile for Drug Delivery to Articular Joints |
| title_full_unstemmed | Chitosan-Based Nanogels: Synthesis and Toxicity Profile for Drug Delivery to Articular Joints |
| title_short | Chitosan-Based Nanogels: Synthesis and Toxicity Profile for Drug Delivery to Articular Joints |
| title_sort | chitosan based nanogels synthesis and toxicity profile for drug delivery to articular joints |
| topic | nanogel biopolymer hyaluronic acid osteoarthritis biocompatibility cartilage |
| url | https://www.mdpi.com/2079-4991/12/8/1337 |
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