Flexible Multiplane Structured Illumination Microscope with a Four-Camera Detector
Fluorescence microscopy provides an unparalleled tool for imaging biological samples. However, producing high-quality volumetric images quickly and without excessive complexity remains a challenge. Here, we demonstrate a four-camera structured illumination microscope (SIM) capable of simultaneously...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-07-01
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| Series: | Photonics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-6732/9/7/501 |
| _version_ | 1797433174967451648 |
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| author | Karl A. Johnson Daniel Noble Rosa Machado Tristan C. Paul Guy M. Hagen |
| author_facet | Karl A. Johnson Daniel Noble Rosa Machado Tristan C. Paul Guy M. Hagen |
| author_sort | Karl A. Johnson |
| collection | DOAJ |
| description | Fluorescence microscopy provides an unparalleled tool for imaging biological samples. However, producing high-quality volumetric images quickly and without excessive complexity remains a challenge. Here, we demonstrate a four-camera structured illumination microscope (SIM) capable of simultaneously imaging multiple focal planes, allowing for the capture of 3D fluorescent images without any axial movement of the sample. This setup allows for the acquisition of many different 3D imaging modes, including 3D time lapses, high-axial-resolution 3D images, and large 3D mosaics. We imaged mitochondrial motions in live cells, neuronal structure in <i>Drosophila</i> larvae, and imaged up to 130 µm deep into mouse brain tissue. After SIM processing, the resolution measured using one of the four cameras improved from 357 nm to 253 nm when using a 30×/1.05 NA objective. |
| first_indexed | 2024-03-09T10:13:18Z |
| format | Article |
| id | doaj.art-4d84c0ba65a243fab6d669c61167caba |
| institution | Directory Open Access Journal |
| issn | 2304-6732 |
| language | English |
| last_indexed | 2024-03-09T10:13:18Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Photonics |
| spelling | doaj.art-4d84c0ba65a243fab6d669c61167caba2023-12-01T22:35:27ZengMDPI AGPhotonics2304-67322022-07-019750110.3390/photonics9070501Flexible Multiplane Structured Illumination Microscope with a Four-Camera DetectorKarl A. Johnson0Daniel Noble1Rosa Machado2Tristan C. Paul3Guy M. Hagen4UCCS BioFrontiers Center, University of Colorado Colorado Springs, 1420 Austin Bluffs Parkway, Colorado Springs, CO 80918, USAUCCS BioFrontiers Center, University of Colorado Colorado Springs, 1420 Austin Bluffs Parkway, Colorado Springs, CO 80918, USAUCCS BioFrontiers Center, University of Colorado Colorado Springs, 1420 Austin Bluffs Parkway, Colorado Springs, CO 80918, USAUCCS BioFrontiers Center, University of Colorado Colorado Springs, 1420 Austin Bluffs Parkway, Colorado Springs, CO 80918, USAUCCS BioFrontiers Center, University of Colorado Colorado Springs, 1420 Austin Bluffs Parkway, Colorado Springs, CO 80918, USAFluorescence microscopy provides an unparalleled tool for imaging biological samples. However, producing high-quality volumetric images quickly and without excessive complexity remains a challenge. Here, we demonstrate a four-camera structured illumination microscope (SIM) capable of simultaneously imaging multiple focal planes, allowing for the capture of 3D fluorescent images without any axial movement of the sample. This setup allows for the acquisition of many different 3D imaging modes, including 3D time lapses, high-axial-resolution 3D images, and large 3D mosaics. We imaged mitochondrial motions in live cells, neuronal structure in <i>Drosophila</i> larvae, and imaged up to 130 µm deep into mouse brain tissue. After SIM processing, the resolution measured using one of the four cameras improved from 357 nm to 253 nm when using a 30×/1.05 NA objective.https://www.mdpi.com/2304-6732/9/7/501structured illuminationfluorescencebrainmulticamera |
| spellingShingle | Karl A. Johnson Daniel Noble Rosa Machado Tristan C. Paul Guy M. Hagen Flexible Multiplane Structured Illumination Microscope with a Four-Camera Detector Photonics structured illumination fluorescence brain multicamera |
| title | Flexible Multiplane Structured Illumination Microscope with a Four-Camera Detector |
| title_full | Flexible Multiplane Structured Illumination Microscope with a Four-Camera Detector |
| title_fullStr | Flexible Multiplane Structured Illumination Microscope with a Four-Camera Detector |
| title_full_unstemmed | Flexible Multiplane Structured Illumination Microscope with a Four-Camera Detector |
| title_short | Flexible Multiplane Structured Illumination Microscope with a Four-Camera Detector |
| title_sort | flexible multiplane structured illumination microscope with a four camera detector |
| topic | structured illumination fluorescence brain multicamera |
| url | https://www.mdpi.com/2304-6732/9/7/501 |
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