Curcumin and melphalan cotreatment induces cell cycle arrest and apoptosis in MDA-MB-231 breast cancer cells
Abstract Breast cancer is the second most common type of cancer worldwide and the leading cause of cancer death in women. Dietary bioactive compounds may act at different stages of carcinogenesis, including tumor initiation, promotion, and progression. Spices have been used for thousands of years an...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-40535-5 |
| _version_ | 1827723160257036288 |
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| author | Carlos Luan A. Passos Renata Madureira Polinati Christian Ferreira Nathalia Alexia Nascimento dos Santos Daniel Galinis V. Lima Jerson Lima da Silva Eliane Fialho |
| author_facet | Carlos Luan A. Passos Renata Madureira Polinati Christian Ferreira Nathalia Alexia Nascimento dos Santos Daniel Galinis V. Lima Jerson Lima da Silva Eliane Fialho |
| author_sort | Carlos Luan A. Passos |
| collection | DOAJ |
| description | Abstract Breast cancer is the second most common type of cancer worldwide and the leading cause of cancer death in women. Dietary bioactive compounds may act at different stages of carcinogenesis, including tumor initiation, promotion, and progression. Spices have been used for thousands of years and have many bioactive compounds with chemopreventive and chemotherapeutic properties. Curcumin has a multitude of beneficial biological properties, including anti-inflammatory and anticancer effects. This study investigated the effects of cotreatment with curcumin and the chemotherapeutic drug melphalan in cultured MDA-MB-231 breast cancer cells. When used alone, both curcumin and melphalan had a cytotoxic effect on breast cancer cells. Combined treatment with 11.65 µM of curcumin and 93.95 µM of melphalan (CURC/MEL) reduced cell viability by 28.64% and 72.43% after 24 h and 48 h, respectively. CURC/MEL reduced the number of colony-forming units and increased ROS levels by 1.36-fold. CURC/MEL alter cell cycle progression, induce apoptosis, and upregulate caspases-3, -7, and -9, in MDA-MB-231 cells. Cotreatment with curcumin and melphalan have anti-breast cancer cells effects and represent a promising candidate for clinical testing. |
| first_indexed | 2024-03-10T21:55:25Z |
| format | Article |
| id | doaj.art-4d85013fcf734e4890a85f6048eeafa0 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T21:55:25Z |
| publishDate | 2023-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-4d85013fcf734e4890a85f6048eeafa02023-11-19T13:08:36ZengNature PortfolioScientific Reports2045-23222023-08-0113111110.1038/s41598-023-40535-5Curcumin and melphalan cotreatment induces cell cycle arrest and apoptosis in MDA-MB-231 breast cancer cellsCarlos Luan A. Passos0Renata Madureira Polinati1Christian Ferreira2Nathalia Alexia Nascimento dos Santos3Daniel Galinis V. Lima4Jerson Lima da Silva5Eliane Fialho6Functional Foods Laboratory, Nutrition Institute, Federal University of Rio de JaneiroFunctional Foods Laboratory, Nutrition Institute, Federal University of Rio de JaneiroFunctional Foods Laboratory, Nutrition Institute, Federal University of Rio de JaneiroFunctional Foods Laboratory, Nutrition Institute, Federal University of Rio de JaneiroFunctional Foods Laboratory, Nutrition Institute, Federal University of Rio de JaneiroMedical Biochemistry Institute, Federal University of Rio de JaneiroFunctional Foods Laboratory, Nutrition Institute, Federal University of Rio de JaneiroAbstract Breast cancer is the second most common type of cancer worldwide and the leading cause of cancer death in women. Dietary bioactive compounds may act at different stages of carcinogenesis, including tumor initiation, promotion, and progression. Spices have been used for thousands of years and have many bioactive compounds with chemopreventive and chemotherapeutic properties. Curcumin has a multitude of beneficial biological properties, including anti-inflammatory and anticancer effects. This study investigated the effects of cotreatment with curcumin and the chemotherapeutic drug melphalan in cultured MDA-MB-231 breast cancer cells. When used alone, both curcumin and melphalan had a cytotoxic effect on breast cancer cells. Combined treatment with 11.65 µM of curcumin and 93.95 µM of melphalan (CURC/MEL) reduced cell viability by 28.64% and 72.43% after 24 h and 48 h, respectively. CURC/MEL reduced the number of colony-forming units and increased ROS levels by 1.36-fold. CURC/MEL alter cell cycle progression, induce apoptosis, and upregulate caspases-3, -7, and -9, in MDA-MB-231 cells. Cotreatment with curcumin and melphalan have anti-breast cancer cells effects and represent a promising candidate for clinical testing.https://doi.org/10.1038/s41598-023-40535-5 |
| spellingShingle | Carlos Luan A. Passos Renata Madureira Polinati Christian Ferreira Nathalia Alexia Nascimento dos Santos Daniel Galinis V. Lima Jerson Lima da Silva Eliane Fialho Curcumin and melphalan cotreatment induces cell cycle arrest and apoptosis in MDA-MB-231 breast cancer cells Scientific Reports |
| title | Curcumin and melphalan cotreatment induces cell cycle arrest and apoptosis in MDA-MB-231 breast cancer cells |
| title_full | Curcumin and melphalan cotreatment induces cell cycle arrest and apoptosis in MDA-MB-231 breast cancer cells |
| title_fullStr | Curcumin and melphalan cotreatment induces cell cycle arrest and apoptosis in MDA-MB-231 breast cancer cells |
| title_full_unstemmed | Curcumin and melphalan cotreatment induces cell cycle arrest and apoptosis in MDA-MB-231 breast cancer cells |
| title_short | Curcumin and melphalan cotreatment induces cell cycle arrest and apoptosis in MDA-MB-231 breast cancer cells |
| title_sort | curcumin and melphalan cotreatment induces cell cycle arrest and apoptosis in mda mb 231 breast cancer cells |
| url | https://doi.org/10.1038/s41598-023-40535-5 |
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