Localizatlon of expansin-like protein in apoplast of pea (Pisum sativum L.) root nodules during interaction with Rhizobium leguminosarum bv. viciae
During nodule development on pea roots, apoplast undergoes changes in activity of plant cell wall proteins such as expansins (EXPs). Because the accumulation of EXP protein has been correlated with the growth of various plant organs, we investigated using Western Blot and immunolocalization studies...
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2011-01-01
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| Series: | Acta Societatis Botanicorum Poloniae |
| Subjects: | |
| Online Access: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/471 |
| _version_ | 1818411746945138688 |
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| author | Marzena Sujkowska Wojciech Borucki Władysław Golinowski |
| author_facet | Marzena Sujkowska Wojciech Borucki Władysław Golinowski |
| author_sort | Marzena Sujkowska |
| collection | DOAJ |
| description | During nodule development on pea roots, apoplast undergoes changes in activity of plant cell wall proteins such as expansins (EXPs). Because the accumulation of EXP protein has been correlated with the growth of various plant organs, we investigated using Western Blot and immunolocalization studies with antibody against PsEXP1, whether this protein was accumulated in the expanding cells of nodule. Immunoblot results indicated the presence of a 30-kDa band specific for pea root nodules. The EXP proteins content rose during growth of pea root nodules. Expansin(s) protein was localized in nodule apoplast as well as in the infection thread walls. The enhanced amount of expansin-like proteins in meristematic part of nodule, root and shoot was shown. The localization of this protein in the meristematic cell walls can be related to the loosening of plant cell wall before cell enlargement. Both, plant cell enlargement and infection thread growth require activity of expansin(s). Possible involvement of EXPs in the process of pea root nodule development is also discussed. |
| first_indexed | 2024-12-14T10:36:19Z |
| format | Article |
| id | doaj.art-4d8803c2fb3d4998b6e87c6c79fb15ff |
| institution | Directory Open Access Journal |
| issn | 2083-9480 |
| language | English |
| last_indexed | 2024-12-14T10:36:19Z |
| publishDate | 2011-01-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Societatis Botanicorum Poloniae |
| spelling | doaj.art-4d8803c2fb3d4998b6e87c6c79fb15ff2022-12-21T23:05:55ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802011-01-01761172610.5586/asbp.2007.002279Localizatlon of expansin-like protein in apoplast of pea (Pisum sativum L.) root nodules during interaction with Rhizobium leguminosarum bv. viciaeMarzena Sujkowska0Wojciech Borucki1Władysław Golinowski2Agricultural University of WarsawAgricultural University of WarsawAgricultural University of WarsawDuring nodule development on pea roots, apoplast undergoes changes in activity of plant cell wall proteins such as expansins (EXPs). Because the accumulation of EXP protein has been correlated with the growth of various plant organs, we investigated using Western Blot and immunolocalization studies with antibody against PsEXP1, whether this protein was accumulated in the expanding cells of nodule. Immunoblot results indicated the presence of a 30-kDa band specific for pea root nodules. The EXP proteins content rose during growth of pea root nodules. Expansin(s) protein was localized in nodule apoplast as well as in the infection thread walls. The enhanced amount of expansin-like proteins in meristematic part of nodule, root and shoot was shown. The localization of this protein in the meristematic cell walls can be related to the loosening of plant cell wall before cell enlargement. Both, plant cell enlargement and infection thread growth require activity of expansin(s). Possible involvement of EXPs in the process of pea root nodule development is also discussed.https://pbsociety.org.pl/journals/index.php/asbp/article/view/471expansinexpansive growthinfection threadsnodulesPisum sativum L.symbiosis |
| spellingShingle | Marzena Sujkowska Wojciech Borucki Władysław Golinowski Localizatlon of expansin-like protein in apoplast of pea (Pisum sativum L.) root nodules during interaction with Rhizobium leguminosarum bv. viciae Acta Societatis Botanicorum Poloniae expansin expansive growth infection threads nodules Pisum sativum L. symbiosis |
| title | Localizatlon of expansin-like protein in apoplast of pea (Pisum sativum L.) root nodules during interaction with Rhizobium leguminosarum bv. viciae |
| title_full | Localizatlon of expansin-like protein in apoplast of pea (Pisum sativum L.) root nodules during interaction with Rhizobium leguminosarum bv. viciae |
| title_fullStr | Localizatlon of expansin-like protein in apoplast of pea (Pisum sativum L.) root nodules during interaction with Rhizobium leguminosarum bv. viciae |
| title_full_unstemmed | Localizatlon of expansin-like protein in apoplast of pea (Pisum sativum L.) root nodules during interaction with Rhizobium leguminosarum bv. viciae |
| title_short | Localizatlon of expansin-like protein in apoplast of pea (Pisum sativum L.) root nodules during interaction with Rhizobium leguminosarum bv. viciae |
| title_sort | localizatlon of expansin like protein in apoplast of pea pisum sativum l root nodules during interaction with rhizobium leguminosarum bv viciae |
| topic | expansin expansive growth infection threads nodules Pisum sativum L. symbiosis |
| url | https://pbsociety.org.pl/journals/index.php/asbp/article/view/471 |
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