Deep metagenomic characterization of gut microbial community and function in preeclampsia
Preeclampsia (PE) is a pregnancy complication characterized by severe hypertension and multiple organ damage. Gut microbiota has been linked to PE by previous amplicon sequencing studies. To resolve the PE gut microbiota in a higher taxonomy resolution, we performed shotgun metagenomic sequencing on...
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| Format: | Article |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.933523/full |
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| author | Li-Juan Lv Sheng-Hui Li Ji-Ying Wen Guang-Yang Wang Hui Li Tian-Wen He Qing-Bo Lv Man-Chun Xiao Hong-Li Duan Min-Chai Chen Zhou-Ting Yi Qiu-Long Yan Ai-Hua Yin |
| author_facet | Li-Juan Lv Sheng-Hui Li Ji-Ying Wen Guang-Yang Wang Hui Li Tian-Wen He Qing-Bo Lv Man-Chun Xiao Hong-Li Duan Min-Chai Chen Zhou-Ting Yi Qiu-Long Yan Ai-Hua Yin |
| author_sort | Li-Juan Lv |
| collection | DOAJ |
| description | Preeclampsia (PE) is a pregnancy complication characterized by severe hypertension and multiple organ damage. Gut microbiota has been linked to PE by previous amplicon sequencing studies. To resolve the PE gut microbiota in a higher taxonomy resolution, we performed shotgun metagenomic sequencing on the fecal samples from 40 early-onset PE and 37 healthy pregnant women. We recovered 1,750 metagenome-assembled genomes (representing 406 species) from the metagenomic dataset and profiled their abundances. We found that PE gut microbiota had enriched in some species belonging to Blautia, Pauljensenia, Ruminococcus, and Collinsella and microbial functions such as the bacitracin/lantibiotics transport system, maltooligosaccharide transport system, multidrug efflux pump, and rhamnose transport system. Conversely, the gut microbiome of healthy pregnant women was enriched in species of Bacteroides and Phocaeicola and microbial functions including the porphyrin and chlorophyll metabolism, pyridoxal-P biosynthesis, riboflavin metabolism, and folate biosynthesis pathway. PE diagnostic potential of gut microbial biomarkers was developed using both species and function profile data. These results will help to explore the relationships between gut bacteria and PE and provide new insights into PE early warning. |
| first_indexed | 2024-04-12T21:48:35Z |
| format | Article |
| id | doaj.art-4d88f3ba338c431ba87970827b608751 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-12T21:48:35Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-4d88f3ba338c431ba87970827b6087512022-12-22T03:15:33ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-09-011210.3389/fcimb.2022.933523933523Deep metagenomic characterization of gut microbial community and function in preeclampsiaLi-Juan Lv0Sheng-Hui Li1Ji-Ying Wen2Guang-Yang Wang3Hui Li4Tian-Wen He5Qing-Bo Lv6Man-Chun Xiao7Hong-Li Duan8Min-Chai Chen9Zhou-Ting Yi10Qiu-Long Yan11Ai-Hua Yin12Medical Genetic Center, Guangdong Women and Children Hospital, Guangzhou, ChinaPuensum Genetech Institute, Wuhan, ChinaDepartment of Obstetric, Guangdong Women and Children Hospital, Guangzhou, ChinaDepartment of Microbiology, College of Basic Medical Sciences, Dalian Medical University, Dalian, ChinaDepartment of Obstetric, Guangdong Women and Children Hospital, Guangzhou, ChinaMedical Genetic Center, Guangdong Women and Children Hospital, Guangzhou, ChinaPuensum Genetech Institute, Wuhan, ChinaDepartment of Microbiology, College of Basic Medical Sciences, Dalian Medical University, Dalian, ChinaDepartment of Obstetric, Guangdong Women and Children Hospital, Guangzhou, ChinaMedical Genetic Center, Guangdong Women and Children Hospital, Guangzhou, ChinaDepartment of Obstetric, Guangdong Women and Children Hospital, Guangzhou, ChinaDepartment of Microbiology, College of Basic Medical Sciences, Dalian Medical University, Dalian, ChinaMedical Genetic Center, Guangdong Women and Children Hospital, Guangzhou, ChinaPreeclampsia (PE) is a pregnancy complication characterized by severe hypertension and multiple organ damage. Gut microbiota has been linked to PE by previous amplicon sequencing studies. To resolve the PE gut microbiota in a higher taxonomy resolution, we performed shotgun metagenomic sequencing on the fecal samples from 40 early-onset PE and 37 healthy pregnant women. We recovered 1,750 metagenome-assembled genomes (representing 406 species) from the metagenomic dataset and profiled their abundances. We found that PE gut microbiota had enriched in some species belonging to Blautia, Pauljensenia, Ruminococcus, and Collinsella and microbial functions such as the bacitracin/lantibiotics transport system, maltooligosaccharide transport system, multidrug efflux pump, and rhamnose transport system. Conversely, the gut microbiome of healthy pregnant women was enriched in species of Bacteroides and Phocaeicola and microbial functions including the porphyrin and chlorophyll metabolism, pyridoxal-P biosynthesis, riboflavin metabolism, and folate biosynthesis pathway. PE diagnostic potential of gut microbial biomarkers was developed using both species and function profile data. These results will help to explore the relationships between gut bacteria and PE and provide new insights into PE early warning.https://www.frontiersin.org/articles/10.3389/fcimb.2022.933523/fullpreeclampsiagut microbiomeshotgun metagenomic sequencingpregnancymicrobial dysbiosismicrobial function |
| spellingShingle | Li-Juan Lv Sheng-Hui Li Ji-Ying Wen Guang-Yang Wang Hui Li Tian-Wen He Qing-Bo Lv Man-Chun Xiao Hong-Li Duan Min-Chai Chen Zhou-Ting Yi Qiu-Long Yan Ai-Hua Yin Deep metagenomic characterization of gut microbial community and function in preeclampsia Frontiers in Cellular and Infection Microbiology preeclampsia gut microbiome shotgun metagenomic sequencing pregnancy microbial dysbiosis microbial function |
| title | Deep metagenomic characterization of gut microbial community and function in preeclampsia |
| title_full | Deep metagenomic characterization of gut microbial community and function in preeclampsia |
| title_fullStr | Deep metagenomic characterization of gut microbial community and function in preeclampsia |
| title_full_unstemmed | Deep metagenomic characterization of gut microbial community and function in preeclampsia |
| title_short | Deep metagenomic characterization of gut microbial community and function in preeclampsia |
| title_sort | deep metagenomic characterization of gut microbial community and function in preeclampsia |
| topic | preeclampsia gut microbiome shotgun metagenomic sequencing pregnancy microbial dysbiosis microbial function |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.933523/full |
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