<i>SfDicer2</i> RNA Interference Inhibits Molting and Wing Expansion in <i>Sogatella furcifera</i>
Endoribonuclease 2 (Dicer2) is a key nicking endonuclease involved in the small interfering RNA biosynthesis, and it plays important roles in gene regulation and antiviral immunity. The Dicer2 sequence was obtained using the transcriptomic and genomic information of <i>Sogatella furcifera</...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/13/8/677 |
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| author | Qing-Hui Zeng Gui-Yun Long Xi-Bin Yang Ze-Yan Jia Dao-Chao Jin Hong Yang |
| author_facet | Qing-Hui Zeng Gui-Yun Long Xi-Bin Yang Ze-Yan Jia Dao-Chao Jin Hong Yang |
| author_sort | Qing-Hui Zeng |
| collection | DOAJ |
| description | Endoribonuclease 2 (Dicer2) is a key nicking endonuclease involved in the small interfering RNA biosynthesis, and it plays important roles in gene regulation and antiviral immunity. The Dicer2 sequence was obtained using the transcriptomic and genomic information of <i>Sogatella furcifera</i> (Horváth), and the spatiotemporal characteristics and functions of molting and wing expansion regulation were studied using real-time quantitative polymerase chain reaction and RNA interference (RNAi) technology. The expression of <i>SfDicer2</i> fluctuated during the nymphal stage of <i>S. furcifera</i>. Its expression decreased significantly over the course of molting. <i>SfDicer2</i> exhibited the highest transcript level in the nymphal stage and adult fat body. After <i>SfDicer2</i> was silenced, the total mortality rate was 42.69%; 18.32% of the insects died because of their inability to molt. Compared with the effects of ds<i>GFP</i> or water, 44.38% of the insects subjected to the silencing of <i>SfDicer2</i> exhibited wing deformities after successful eclosion. After <i>SfDicer2</i> RNAi, the expression of chitinase, chitin deacetylase, trehalase, chitin synthase 1, and wing expansion-related genes was significantly inhibited. These findings indicate that <i>SfDicer2</i> controls molting by affecting genes associated with chitin synthesis and degradation and regulates wing expansion by altering the expression of wing expansion-related genes in <i>S. furcifera</i>. |
| first_indexed | 2024-03-09T09:55:15Z |
| format | Article |
| id | doaj.art-4d8a78d07f2a41d5a82c54d3c6c4f886 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T09:55:15Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-4d8a78d07f2a41d5a82c54d3c6c4f8862023-12-01T23:49:23ZengMDPI AGInsects2075-44502022-07-0113867710.3390/insects13080677<i>SfDicer2</i> RNA Interference Inhibits Molting and Wing Expansion in <i>Sogatella furcifera</i>Qing-Hui Zeng0Gui-Yun Long1Xi-Bin Yang2Ze-Yan Jia3Dao-Chao Jin4Hong Yang5Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Scientific Observing and Experimental Station of Crop Pests in Guiyang, Ministry of Agriculture and Rural Affairs, Institute of Entomology, Guizhou University, Guiyang 550025, ChinaGuizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Scientific Observing and Experimental Station of Crop Pests in Guiyang, Ministry of Agriculture and Rural Affairs, Institute of Entomology, Guizhou University, Guiyang 550025, ChinaGuizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Scientific Observing and Experimental Station of Crop Pests in Guiyang, Ministry of Agriculture and Rural Affairs, Institute of Entomology, Guizhou University, Guiyang 550025, ChinaGuizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Scientific Observing and Experimental Station of Crop Pests in Guiyang, Ministry of Agriculture and Rural Affairs, Institute of Entomology, Guizhou University, Guiyang 550025, ChinaGuizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Scientific Observing and Experimental Station of Crop Pests in Guiyang, Ministry of Agriculture and Rural Affairs, Institute of Entomology, Guizhou University, Guiyang 550025, ChinaGuizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Scientific Observing and Experimental Station of Crop Pests in Guiyang, Ministry of Agriculture and Rural Affairs, Institute of Entomology, Guizhou University, Guiyang 550025, ChinaEndoribonuclease 2 (Dicer2) is a key nicking endonuclease involved in the small interfering RNA biosynthesis, and it plays important roles in gene regulation and antiviral immunity. The Dicer2 sequence was obtained using the transcriptomic and genomic information of <i>Sogatella furcifera</i> (Horváth), and the spatiotemporal characteristics and functions of molting and wing expansion regulation were studied using real-time quantitative polymerase chain reaction and RNA interference (RNAi) technology. The expression of <i>SfDicer2</i> fluctuated during the nymphal stage of <i>S. furcifera</i>. Its expression decreased significantly over the course of molting. <i>SfDicer2</i> exhibited the highest transcript level in the nymphal stage and adult fat body. After <i>SfDicer2</i> was silenced, the total mortality rate was 42.69%; 18.32% of the insects died because of their inability to molt. Compared with the effects of ds<i>GFP</i> or water, 44.38% of the insects subjected to the silencing of <i>SfDicer2</i> exhibited wing deformities after successful eclosion. After <i>SfDicer2</i> RNAi, the expression of chitinase, chitin deacetylase, trehalase, chitin synthase 1, and wing expansion-related genes was significantly inhibited. These findings indicate that <i>SfDicer2</i> controls molting by affecting genes associated with chitin synthesis and degradation and regulates wing expansion by altering the expression of wing expansion-related genes in <i>S. furcifera</i>.https://www.mdpi.com/2075-4450/13/8/677<i>Sogatella furcifera</i><i>Dicer2</i>RNA interferencemoltingwing expansion |
| spellingShingle | Qing-Hui Zeng Gui-Yun Long Xi-Bin Yang Ze-Yan Jia Dao-Chao Jin Hong Yang <i>SfDicer2</i> RNA Interference Inhibits Molting and Wing Expansion in <i>Sogatella furcifera</i> Insects <i>Sogatella furcifera</i> <i>Dicer2</i> RNA interference molting wing expansion |
| title | <i>SfDicer2</i> RNA Interference Inhibits Molting and Wing Expansion in <i>Sogatella furcifera</i> |
| title_full | <i>SfDicer2</i> RNA Interference Inhibits Molting and Wing Expansion in <i>Sogatella furcifera</i> |
| title_fullStr | <i>SfDicer2</i> RNA Interference Inhibits Molting and Wing Expansion in <i>Sogatella furcifera</i> |
| title_full_unstemmed | <i>SfDicer2</i> RNA Interference Inhibits Molting and Wing Expansion in <i>Sogatella furcifera</i> |
| title_short | <i>SfDicer2</i> RNA Interference Inhibits Molting and Wing Expansion in <i>Sogatella furcifera</i> |
| title_sort | i sfdicer2 i rna interference inhibits molting and wing expansion in i sogatella furcifera i |
| topic | <i>Sogatella furcifera</i> <i>Dicer2</i> RNA interference molting wing expansion |
| url | https://www.mdpi.com/2075-4450/13/8/677 |
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