Hormonal Responses to Susceptible, Intermediate, and Resistant Interactions in the <i>Brassica napus</i>–<i>Leptosphaeria maculans</i> Pathosystem
Hormone signaling plays a pivotal role in plant–microbe interactions. There are three major phytohormones in plant defense: salicylic acid (SA), jasmonic acid (JA), and ethylene (ET). The activation and trade-off of signaling between these three hormones likely determines the strength of plant defen...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/9/4714 |
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| author | Cunchun Yang W. G. Dilantha Fernando |
| author_facet | Cunchun Yang W. G. Dilantha Fernando |
| author_sort | Cunchun Yang |
| collection | DOAJ |
| description | Hormone signaling plays a pivotal role in plant–microbe interactions. There are three major phytohormones in plant defense: salicylic acid (SA), jasmonic acid (JA), and ethylene (ET). The activation and trade-off of signaling between these three hormones likely determines the strength of plant defense in response to pathogens. Here, we describe the allocation of hormonal signaling in <i>Brassica napus</i> against the fungal pathogen <i>Leptosphaeria maculans</i>. Three <i>B. napus</i> genotypes (Westar, Surpass400, and 01-23-2-1) were inoculated with two <i>L. maculans</i> isolates (H75 8-1 and H77 7-2), subsequently exhibiting three levels of resistance: susceptible, intermediate, and resistant. Quantitative analyses suggest that the early activation of some SA-responsive genes, including <i>WRKY70</i> and <i>NPR1</i>, contribute to an effective defense against <i>L. maculans</i>. The co-expression among factors responding to SA/ET/JA was also observed in the late stage of infection. The results of conjugated SA measurement also support that early SA activation plays a crucial role in durable resistance. Our results demonstrate the relationship between the onset patterns of certain hormone regulators and the effectiveness of the defense of <i>B. napus</i> against <i>L. maculans</i>. |
| first_indexed | 2024-03-10T11:50:03Z |
| format | Article |
| id | doaj.art-4d8be3c7b20a444c8caaa6c13c30362c |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T11:50:03Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4d8be3c7b20a444c8caaa6c13c30362c2023-11-21T17:45:34ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-04-01229471410.3390/ijms22094714Hormonal Responses to Susceptible, Intermediate, and Resistant Interactions in the <i>Brassica napus</i>–<i>Leptosphaeria maculans</i> PathosystemCunchun Yang0W. G. Dilantha Fernando1Department of Plant Science, Faculty of Agriculture and Food Science, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaDepartment of Plant Science, Faculty of Agriculture and Food Science, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaHormone signaling plays a pivotal role in plant–microbe interactions. There are three major phytohormones in plant defense: salicylic acid (SA), jasmonic acid (JA), and ethylene (ET). The activation and trade-off of signaling between these three hormones likely determines the strength of plant defense in response to pathogens. Here, we describe the allocation of hormonal signaling in <i>Brassica napus</i> against the fungal pathogen <i>Leptosphaeria maculans</i>. Three <i>B. napus</i> genotypes (Westar, Surpass400, and 01-23-2-1) were inoculated with two <i>L. maculans</i> isolates (H75 8-1 and H77 7-2), subsequently exhibiting three levels of resistance: susceptible, intermediate, and resistant. Quantitative analyses suggest that the early activation of some SA-responsive genes, including <i>WRKY70</i> and <i>NPR1</i>, contribute to an effective defense against <i>L. maculans</i>. The co-expression among factors responding to SA/ET/JA was also observed in the late stage of infection. The results of conjugated SA measurement also support that early SA activation plays a crucial role in durable resistance. Our results demonstrate the relationship between the onset patterns of certain hormone regulators and the effectiveness of the defense of <i>B. napus</i> against <i>L. maculans</i>.https://www.mdpi.com/1422-0067/22/9/4714<i>Leptosphaeria maculans</i><i>Brassica napus</i>hormone signalinggene expressionsalicylic acid (SA)jasmonic acid (JA) |
| spellingShingle | Cunchun Yang W. G. Dilantha Fernando Hormonal Responses to Susceptible, Intermediate, and Resistant Interactions in the <i>Brassica napus</i>–<i>Leptosphaeria maculans</i> Pathosystem International Journal of Molecular Sciences <i>Leptosphaeria maculans</i> <i>Brassica napus</i> hormone signaling gene expression salicylic acid (SA) jasmonic acid (JA) |
| title | Hormonal Responses to Susceptible, Intermediate, and Resistant Interactions in the <i>Brassica napus</i>–<i>Leptosphaeria maculans</i> Pathosystem |
| title_full | Hormonal Responses to Susceptible, Intermediate, and Resistant Interactions in the <i>Brassica napus</i>–<i>Leptosphaeria maculans</i> Pathosystem |
| title_fullStr | Hormonal Responses to Susceptible, Intermediate, and Resistant Interactions in the <i>Brassica napus</i>–<i>Leptosphaeria maculans</i> Pathosystem |
| title_full_unstemmed | Hormonal Responses to Susceptible, Intermediate, and Resistant Interactions in the <i>Brassica napus</i>–<i>Leptosphaeria maculans</i> Pathosystem |
| title_short | Hormonal Responses to Susceptible, Intermediate, and Resistant Interactions in the <i>Brassica napus</i>–<i>Leptosphaeria maculans</i> Pathosystem |
| title_sort | hormonal responses to susceptible intermediate and resistant interactions in the i brassica napus i i leptosphaeria maculans i pathosystem |
| topic | <i>Leptosphaeria maculans</i> <i>Brassica napus</i> hormone signaling gene expression salicylic acid (SA) jasmonic acid (JA) |
| url | https://www.mdpi.com/1422-0067/22/9/4714 |
| work_keys_str_mv | AT cunchunyang hormonalresponsestosusceptibleintermediateandresistantinteractionsintheibrassicanapusiileptosphaeriamaculansipathosystem AT wgdilanthafernando hormonalresponsestosusceptibleintermediateandresistantinteractionsintheibrassicanapusiileptosphaeriamaculansipathosystem |