Microorganisms Involved in Hydrogen Sink in the Gastrointestinal Tract of Chickens
Hydrogen sink is a beneficial process, which has never been properly examined in chickens. Therefore, the aim of this study was to assess the quantity and quality of microbiota involved in hydrogen uptake with the use of real-time PCR and metagenome sequencing. Analyses were carried out in 50 free-r...
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MDPI AG
2023-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/7/6674 |
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| author | Agata Anna Cisek Beata Dolka Iwona Bąk Bożena Cukrowska |
| author_facet | Agata Anna Cisek Beata Dolka Iwona Bąk Bożena Cukrowska |
| author_sort | Agata Anna Cisek |
| collection | DOAJ |
| description | Hydrogen sink is a beneficial process, which has never been properly examined in chickens. Therefore, the aim of this study was to assess the quantity and quality of microbiota involved in hydrogen uptake with the use of real-time PCR and metagenome sequencing. Analyses were carried out in 50 free-range chickens, 50 commercial broilers, and 54 experimental chickens isolated from external factors. The median values of acetogens, methanogens, sulfate-reducing bacteria (SRB), and [NiFe]-hydrogenase utilizers measured in the cecum were approx. 7.6, 0, 0, and 3.2 log<sub>10</sub>/gram of wet weight, respectively. For the excreta samples, these values were 5.9, 4.8, 4, and 3 log<sub>10</sub>/gram of wet weight, respectively. Our results showed that the acetogens were dominant over the other tested groups of hydrogen consumers. The quantities of methanogens, SRB, and the [NiFe]-hydrogenase utilizers were dependent on the overall rearing conditions, being the result of diet, environment, agrotechnical measures, and other factors combined. By sequencing of the 16S rRNA gene, archaea of the genus <i>Methanomassiliicoccus</i> (<i>Candidatus Methanomassiliicoccus</i>) were discovered in chickens for the first time. This study provides some indication that in chickens, acetogenesis may be the main metabolic pathway responsible for hydrogen sink. |
| first_indexed | 2024-03-11T05:33:43Z |
| format | Article |
| id | doaj.art-4d8ee468f7154fcaaf2eb90c8bc51cfe |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T05:33:43Z |
| publishDate | 2023-04-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4d8ee468f7154fcaaf2eb90c8bc51cfe2023-11-17T16:54:27ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-04-01247667410.3390/ijms24076674Microorganisms Involved in Hydrogen Sink in the Gastrointestinal Tract of ChickensAgata Anna Cisek0Beata Dolka1Iwona Bąk2Bożena Cukrowska3Department of Pathology, The Children’s Memorial Health Institute, Av. Dzieci Polskich 20, 04-730 Warsaw, PolandDepartment of Pathology and Veterinary Diagnostics, Institute of Veterinary Medicine, Warsaw University of Life Sciences, St. Ciszewskiego 8, 02-786 Warsaw, PolandDepartment of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences, St. Ciszewskiego 8, 02-786 Warsaw, PolandDepartment of Pathology, The Children’s Memorial Health Institute, Av. Dzieci Polskich 20, 04-730 Warsaw, PolandHydrogen sink is a beneficial process, which has never been properly examined in chickens. Therefore, the aim of this study was to assess the quantity and quality of microbiota involved in hydrogen uptake with the use of real-time PCR and metagenome sequencing. Analyses were carried out in 50 free-range chickens, 50 commercial broilers, and 54 experimental chickens isolated from external factors. The median values of acetogens, methanogens, sulfate-reducing bacteria (SRB), and [NiFe]-hydrogenase utilizers measured in the cecum were approx. 7.6, 0, 0, and 3.2 log<sub>10</sub>/gram of wet weight, respectively. For the excreta samples, these values were 5.9, 4.8, 4, and 3 log<sub>10</sub>/gram of wet weight, respectively. Our results showed that the acetogens were dominant over the other tested groups of hydrogen consumers. The quantities of methanogens, SRB, and the [NiFe]-hydrogenase utilizers were dependent on the overall rearing conditions, being the result of diet, environment, agrotechnical measures, and other factors combined. By sequencing of the 16S rRNA gene, archaea of the genus <i>Methanomassiliicoccus</i> (<i>Candidatus Methanomassiliicoccus</i>) were discovered in chickens for the first time. This study provides some indication that in chickens, acetogenesis may be the main metabolic pathway responsible for hydrogen sink.https://www.mdpi.com/1422-0067/24/7/6674acetogens<i>Campylobacter jejuni</i>hydrogen uptakemethanogenic archaea<i>Methanomassiliicoccus</i>chicken gut microbiota |
| spellingShingle | Agata Anna Cisek Beata Dolka Iwona Bąk Bożena Cukrowska Microorganisms Involved in Hydrogen Sink in the Gastrointestinal Tract of Chickens International Journal of Molecular Sciences acetogens <i>Campylobacter jejuni</i> hydrogen uptake methanogenic archaea <i>Methanomassiliicoccus</i> chicken gut microbiota |
| title | Microorganisms Involved in Hydrogen Sink in the Gastrointestinal Tract of Chickens |
| title_full | Microorganisms Involved in Hydrogen Sink in the Gastrointestinal Tract of Chickens |
| title_fullStr | Microorganisms Involved in Hydrogen Sink in the Gastrointestinal Tract of Chickens |
| title_full_unstemmed | Microorganisms Involved in Hydrogen Sink in the Gastrointestinal Tract of Chickens |
| title_short | Microorganisms Involved in Hydrogen Sink in the Gastrointestinal Tract of Chickens |
| title_sort | microorganisms involved in hydrogen sink in the gastrointestinal tract of chickens |
| topic | acetogens <i>Campylobacter jejuni</i> hydrogen uptake methanogenic archaea <i>Methanomassiliicoccus</i> chicken gut microbiota |
| url | https://www.mdpi.com/1422-0067/24/7/6674 |
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