Detection of multi-drug resistant (MDR) Escherichia coli and tet gene prevalence at a pig farm in Kupang, Indonesia
Objective: The purpose of this study was to detect the incidence of multi-drug resistant (MDR) and the spread of tet genes that encode tetracycline (TE) resistance in E. coli in pig farms in the city of Kupang, Indonesia. Materials and Methods: Samples of pig feces have been obtained from 96 pig far...
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| Format: | Article |
| Language: | English |
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Network for the Veterinarians of Bangladesh
2018-12-01
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| Series: | Journal of Advanced Veterinary and Animal Research |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=298393 |
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| author | Novalino Harold Geoffrey Kallau I Wayan Teguh Wibawan Denny Widaya Lukman Mirnawati Bachrum Sudarwanto |
| author_facet | Novalino Harold Geoffrey Kallau I Wayan Teguh Wibawan Denny Widaya Lukman Mirnawati Bachrum Sudarwanto |
| author_sort | Novalino Harold Geoffrey Kallau |
| collection | DOAJ |
| description | Objective: The purpose of this study was to detect the incidence of multi-drug resistant (MDR) and the spread of tet genes that encode tetracycline (TE) resistance in E. coli in pig farms in the city of Kupang, Indonesia.
Materials and Methods: Samples of pig feces have been obtained from 96 pig farms in Kupang city, Indonesia. Escherichia coli bacteria were isolated and identified morphologically and biochemically, and finally confirmed by the API test. The disk diffusion method has been used to observe the antibiotic sensitivity effects and has been followed by observing resistant genes encoding TE resistance using the multiplex polymerase chain reaction (m-PCR) method to detect the presence of tet genes such as tet (A), tet (B), tet (C), tet (D), and tet (E), respectively.
Results: A total of 82 (85.4%) of E. coli isolates have been found in all pig feces samples obtained from 96 pig farms in Kupang city. This study has shown a high level of antibiotic resistance dominated by erythromycin (85.4%) and cephalothin (58.5%) and followed by several other antibiotics with a percentage below 34.1%. The prevalence of MDR E. coli was 57.3% by showing 39 different patterns. The most common pattern was showed by the Cephalothin-Colistin-Erythromycin pattern. The resistance of E. coli to TE appears to be related to the presence of tet (A) and tet (E) genes.
Conclusion: This study has encouraged the need for public awareness (farmers) of the wise use of antibiotics in preventing the spread of resistant bacteria that can cause health problems in animals and humans. [J Adv Vet Anim Res 2018; 5(4.000): 388-396] |
| first_indexed | 2024-12-11T10:04:06Z |
| format | Article |
| id | doaj.art-4d95c488a8414cce846713a115cb7cc3 |
| institution | Directory Open Access Journal |
| issn | 2311-7710 |
| language | English |
| last_indexed | 2024-12-11T10:04:06Z |
| publishDate | 2018-12-01 |
| publisher | Network for the Veterinarians of Bangladesh |
| record_format | Article |
| series | Journal of Advanced Veterinary and Animal Research |
| spelling | doaj.art-4d95c488a8414cce846713a115cb7cc32022-12-22T01:12:00ZengNetwork for the Veterinarians of BangladeshJournal of Advanced Veterinary and Animal Research2311-77102018-12-015438839610.5455/javar.2018.e289298393Detection of multi-drug resistant (MDR) Escherichia coli and tet gene prevalence at a pig farm in Kupang, IndonesiaNovalino Harold Geoffrey Kallau0I Wayan Teguh Wibawan1Denny Widaya Lukman2Mirnawati Bachrum Sudarwanto3Department of Animal Disease and Veterinary Public Health, Faculty of Veterinary Medicine of Nusa Cendana University of Indonesia Department of Animal Disease and Veterinary Public Health, Faculty of Veterinary Medicine of Bogor Agricultural Institute Indonesia Department of Animal Disease and Veterinary Public Health, Faculty of Veterinary Medicine of Bogor Agricultural Institute Indonesia Department of Animal Disease and Veterinary Public Health, Faculty of Veterinary Medicine of Bogor Agricultural Institute IndonesiaObjective: The purpose of this study was to detect the incidence of multi-drug resistant (MDR) and the spread of tet genes that encode tetracycline (TE) resistance in E. coli in pig farms in the city of Kupang, Indonesia. Materials and Methods: Samples of pig feces have been obtained from 96 pig farms in Kupang city, Indonesia. Escherichia coli bacteria were isolated and identified morphologically and biochemically, and finally confirmed by the API test. The disk diffusion method has been used to observe the antibiotic sensitivity effects and has been followed by observing resistant genes encoding TE resistance using the multiplex polymerase chain reaction (m-PCR) method to detect the presence of tet genes such as tet (A), tet (B), tet (C), tet (D), and tet (E), respectively. Results: A total of 82 (85.4%) of E. coli isolates have been found in all pig feces samples obtained from 96 pig farms in Kupang city. This study has shown a high level of antibiotic resistance dominated by erythromycin (85.4%) and cephalothin (58.5%) and followed by several other antibiotics with a percentage below 34.1%. The prevalence of MDR E. coli was 57.3% by showing 39 different patterns. The most common pattern was showed by the Cephalothin-Colistin-Erythromycin pattern. The resistance of E. coli to TE appears to be related to the presence of tet (A) and tet (E) genes. Conclusion: This study has encouraged the need for public awareness (farmers) of the wise use of antibiotics in preventing the spread of resistant bacteria that can cause health problems in animals and humans. [J Adv Vet Anim Res 2018; 5(4.000): 388-396]http://www.ejmanager.com/fulltextpdf.php?mno=298393Antibiotics; E. coli; tet gene; multidrug resistance; pig farm |
| spellingShingle | Novalino Harold Geoffrey Kallau I Wayan Teguh Wibawan Denny Widaya Lukman Mirnawati Bachrum Sudarwanto Detection of multi-drug resistant (MDR) Escherichia coli and tet gene prevalence at a pig farm in Kupang, Indonesia Journal of Advanced Veterinary and Animal Research Antibiotics; E. coli; tet gene; multidrug resistance; pig farm |
| title | Detection of multi-drug resistant (MDR) Escherichia coli and tet gene prevalence at a pig farm in Kupang, Indonesia |
| title_full | Detection of multi-drug resistant (MDR) Escherichia coli and tet gene prevalence at a pig farm in Kupang, Indonesia |
| title_fullStr | Detection of multi-drug resistant (MDR) Escherichia coli and tet gene prevalence at a pig farm in Kupang, Indonesia |
| title_full_unstemmed | Detection of multi-drug resistant (MDR) Escherichia coli and tet gene prevalence at a pig farm in Kupang, Indonesia |
| title_short | Detection of multi-drug resistant (MDR) Escherichia coli and tet gene prevalence at a pig farm in Kupang, Indonesia |
| title_sort | detection of multi drug resistant mdr escherichia coli and tet gene prevalence at a pig farm in kupang indonesia |
| topic | Antibiotics; E. coli; tet gene; multidrug resistance; pig farm |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=298393 |
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