Reorganization of Metabolism during Cardiomyogenesis Implies Time-Specific Signaling Pathway Regulation
Understanding the cell differentiation process involves the characterization of signaling and regulatory pathways. The coordinated action involved in multilevel regulation determines the commitment of stem cells and their differentiation into a specific cell lineage. Cellular metabolism plays a rele...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/3/1330 |
| _version_ | 1797406096595353600 |
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| author | María Julia Barisón Isabela Tiemy Pereira Anny Waloski Robert Bruno Dallagiovanna |
| author_facet | María Julia Barisón Isabela Tiemy Pereira Anny Waloski Robert Bruno Dallagiovanna |
| author_sort | María Julia Barisón |
| collection | DOAJ |
| description | Understanding the cell differentiation process involves the characterization of signaling and regulatory pathways. The coordinated action involved in multilevel regulation determines the commitment of stem cells and their differentiation into a specific cell lineage. Cellular metabolism plays a relevant role in modulating the expression of genes, which act as sensors of the extra-and intracellular environment. In this work, we analyzed mRNAs associated with polysomes by focusing on the expression profile of metabolism-related genes during the cardiac differentiation of human embryonic stem cells (hESCs). We compared different time points during cardiac differentiation (pluripotency, embryoid body aggregation, cardiac mesoderm, cardiac progenitor and cardiomyocyte) and showed the immature cell profile of energy metabolism. Highly regulated canonical pathways are thoroughly discussed, such as those involved in metabolic signaling and lipid homeostasis. We reveal the critical relevance of retinoic X receptor (RXR) heterodimers in upstream retinoic acid metabolism and their relationship with thyroid hormone signaling. Additionally, we highlight the importance of lipid homeostasis and extracellular matrix component biosynthesis during cardiomyogenesis, providing new insights into how hESCs reorganize their metabolism during in vitro cardiac differentiation. |
| first_indexed | 2024-03-09T03:21:21Z |
| format | Article |
| id | doaj.art-4d9aed3d3ab348d5ae86ae190689c46f |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T03:21:21Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4d9aed3d3ab348d5ae86ae190689c46f2023-12-03T15:09:53ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-01223133010.3390/ijms22031330Reorganization of Metabolism during Cardiomyogenesis Implies Time-Specific Signaling Pathway RegulationMaría Julia Barisón0Isabela Tiemy Pereira1Anny Waloski Robert2Bruno Dallagiovanna3Basic Stem Cell Biology Laboratory, Instituto Carlos Chagas-FIOCRUZ-PR, Rua Professor Algacyr Munhoz Mader, 3775, Curitiba, PR 81350-010, BrazilBasic Stem Cell Biology Laboratory, Instituto Carlos Chagas-FIOCRUZ-PR, Rua Professor Algacyr Munhoz Mader, 3775, Curitiba, PR 81350-010, BrazilBasic Stem Cell Biology Laboratory, Instituto Carlos Chagas-FIOCRUZ-PR, Rua Professor Algacyr Munhoz Mader, 3775, Curitiba, PR 81350-010, BrazilBasic Stem Cell Biology Laboratory, Instituto Carlos Chagas-FIOCRUZ-PR, Rua Professor Algacyr Munhoz Mader, 3775, Curitiba, PR 81350-010, BrazilUnderstanding the cell differentiation process involves the characterization of signaling and regulatory pathways. The coordinated action involved in multilevel regulation determines the commitment of stem cells and their differentiation into a specific cell lineage. Cellular metabolism plays a relevant role in modulating the expression of genes, which act as sensors of the extra-and intracellular environment. In this work, we analyzed mRNAs associated with polysomes by focusing on the expression profile of metabolism-related genes during the cardiac differentiation of human embryonic stem cells (hESCs). We compared different time points during cardiac differentiation (pluripotency, embryoid body aggregation, cardiac mesoderm, cardiac progenitor and cardiomyocyte) and showed the immature cell profile of energy metabolism. Highly regulated canonical pathways are thoroughly discussed, such as those involved in metabolic signaling and lipid homeostasis. We reveal the critical relevance of retinoic X receptor (RXR) heterodimers in upstream retinoic acid metabolism and their relationship with thyroid hormone signaling. Additionally, we highlight the importance of lipid homeostasis and extracellular matrix component biosynthesis during cardiomyogenesis, providing new insights into how hESCs reorganize their metabolism during in vitro cardiac differentiation.https://www.mdpi.com/1422-0067/22/3/1330hESCcardiomyocytescardiac differentiationmetabolismRXR heterodimersthyroid hormone |
| spellingShingle | María Julia Barisón Isabela Tiemy Pereira Anny Waloski Robert Bruno Dallagiovanna Reorganization of Metabolism during Cardiomyogenesis Implies Time-Specific Signaling Pathway Regulation International Journal of Molecular Sciences hESC cardiomyocytes cardiac differentiation metabolism RXR heterodimers thyroid hormone |
| title | Reorganization of Metabolism during Cardiomyogenesis Implies Time-Specific Signaling Pathway Regulation |
| title_full | Reorganization of Metabolism during Cardiomyogenesis Implies Time-Specific Signaling Pathway Regulation |
| title_fullStr | Reorganization of Metabolism during Cardiomyogenesis Implies Time-Specific Signaling Pathway Regulation |
| title_full_unstemmed | Reorganization of Metabolism during Cardiomyogenesis Implies Time-Specific Signaling Pathway Regulation |
| title_short | Reorganization of Metabolism during Cardiomyogenesis Implies Time-Specific Signaling Pathway Regulation |
| title_sort | reorganization of metabolism during cardiomyogenesis implies time specific signaling pathway regulation |
| topic | hESC cardiomyocytes cardiac differentiation metabolism RXR heterodimers thyroid hormone |
| url | https://www.mdpi.com/1422-0067/22/3/1330 |
| work_keys_str_mv | AT mariajuliabarison reorganizationofmetabolismduringcardiomyogenesisimpliestimespecificsignalingpathwayregulation AT isabelatiemypereira reorganizationofmetabolismduringcardiomyogenesisimpliestimespecificsignalingpathwayregulation AT annywaloskirobert reorganizationofmetabolismduringcardiomyogenesisimpliestimespecificsignalingpathwayregulation AT brunodallagiovanna reorganizationofmetabolismduringcardiomyogenesisimpliestimespecificsignalingpathwayregulation |