Exposure to particulate matter 2.5 leading to lung microbiome disorder and the alleviation effect of Auricularia auricular-judae polysaccharide
Objectives The aim of the paper is to explore the role of lung microbiome disorder in lung tissue injury induced by exposure to particulate matter with a maximum diameter of 2.5 μm (PM2.5) and the alleviation effect of Auricularia auricular-judae polysaccharide (AAP). Material and Methods Sprague...
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| Format: | Article |
| Language: | English |
| Published: |
Nofer Institute of Occupational Medicine
2022-07-01
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| Series: | International Journal of Occupational Medicine and Environmental Health |
| Subjects: | |
| Online Access: | http://ijomeh.eu/Exposure-to-particulate-matter-2-5-leading-to-lung-microbiome-disorder-and-the-alleviation,141469,0,2.html |
| _version_ | 1797980731934244864 |
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| author | Yanshu Zhang Bin He Lei Wu Xiaoyi Mi Lijin Zhang Shuang Li Jian Wang Xiaoyu Yu |
| author_facet | Yanshu Zhang Bin He Lei Wu Xiaoyi Mi Lijin Zhang Shuang Li Jian Wang Xiaoyu Yu |
| author_sort | Yanshu Zhang |
| collection | DOAJ |
| description | Objectives
The aim of the paper is to explore the role of lung microbiome disorder in lung tissue injury induced by exposure to particulate matter
with a maximum diameter of 2.5 μm (PM2.5) and the alleviation effect of Auricularia auricular-judae polysaccharide (AAP).
Material and Methods
Sprague Dawley rats were given PM 2.5 suspension at a dose of 20 mg/l twice a week for 8 weeks. Then, 100 mg/kg or 200 mg/kg of AAP was administered
to the rats after PM 2.5 exposure. The bronchoalveolar lavage fluid (BALF) and lung tissue samples were collected at the end of the experiment.
The BALF was meant to detect changes in lung microbiome by 16S sequences and cluster analysis, with the application of the principal component
analysis and the partial least squares discriminant analysis. The levels of interferon-γ (IFN-γ), and interleukin (IL)-4, IL-8, and IL-10 in lung tissue
were detected by the enzyme-linked immunosorbent assay method. The pathological changes in lung tissue were observed by hematoxylin and
eosin staining.
Results
After PM 2.5 exposure, the alveolar septum was widened, and the structures of alveolar walls were destroyed. There was
inflammatory cells infiltration in the alveolar space and the interstitial space. Alpha diversity in BALF showed that the Chao1, ACE, Simpson, and
Shannon values were increased, and the lung microbiome analysis revealed that the relative abundance of Firmicutes and Clostridium increased,
while the relative abundance of Bacteroidetes and Akkermansia decreased. The contents of IFN-γ and IL-8 in lung tissue increased while the content
of IL-10 decreased. After the administration of AAP, the alveolar structure damage was alleviated, and the interstitial hemorrhage, edema, and inflammatory
cells infiltration were reduced. The Chao1 and ACE values decreased, and the taxonomic abundance values of Akkermansia were much
higher. Simultaneously, the contents of IFN-γ, IL-4, and IL-8 decreased, and the content of IL-10 increased.
Conclusions
It was found that PM 2.5 resulted in lung microbiome disorder, which might lead to the inflammation of lung tissue. It was also revealed that AAP could alleviate the inflammatory damage of lung tissue induced by PM 2.5 . Int J Occup Med Environ Health. 2022;35(6):651–64 |
| first_indexed | 2024-04-11T05:59:34Z |
| format | Article |
| id | doaj.art-4da1009dd8354b53be91bdadef39100c |
| institution | Directory Open Access Journal |
| issn | 1232-1087 1896-494X |
| language | English |
| last_indexed | 2024-04-11T05:59:34Z |
| publishDate | 2022-07-01 |
| publisher | Nofer Institute of Occupational Medicine |
| record_format | Article |
| series | International Journal of Occupational Medicine and Environmental Health |
| spelling | doaj.art-4da1009dd8354b53be91bdadef39100c2022-12-22T04:41:47ZengNofer Institute of Occupational MedicineInternational Journal of Occupational Medicine and Environmental Health1232-10871896-494X2022-07-0135665166410.13075/ijomeh.1896.01742141469Exposure to particulate matter 2.5 leading to lung microbiome disorder and the alleviation effect of Auricularia auricular-judae polysaccharideYanshu Zhang0Bin He1Lei Wu2Xiaoyi Mi3Lijin Zhang4Shuang Li5Jian Wang6Xiaoyu Yu7North China University of Science and Technology, Tangshan, People’s Republic of China (School of Public Health)North China University of Science and Technology, Tangshan, People’s Republic of China (School of Public Health)North China University of Science and Technology, Tangshan, People’s Republic of China (School of Public Health)North China University of Science and Technology, Tangshan, People’s Republic of China (School of Public Health)North China University of Science and Technology, Tangshan, People’s Republic of China (School of Public Health)North China University of Science and Technology, Tangshan, People’s Republic of China (Experiment Animal Center)North China University of Science and Technology, Tangshan, People’s Republic of China (Experiment Animal Center)North China University of Science and Technology, Tangshan, People’s Republic of China (School of Psychology and Mental Health)Objectives The aim of the paper is to explore the role of lung microbiome disorder in lung tissue injury induced by exposure to particulate matter with a maximum diameter of 2.5 μm (PM2.5) and the alleviation effect of Auricularia auricular-judae polysaccharide (AAP). Material and Methods Sprague Dawley rats were given PM 2.5 suspension at a dose of 20 mg/l twice a week for 8 weeks. Then, 100 mg/kg or 200 mg/kg of AAP was administered to the rats after PM 2.5 exposure. The bronchoalveolar lavage fluid (BALF) and lung tissue samples were collected at the end of the experiment. The BALF was meant to detect changes in lung microbiome by 16S sequences and cluster analysis, with the application of the principal component analysis and the partial least squares discriminant analysis. The levels of interferon-γ (IFN-γ), and interleukin (IL)-4, IL-8, and IL-10 in lung tissue were detected by the enzyme-linked immunosorbent assay method. The pathological changes in lung tissue were observed by hematoxylin and eosin staining. Results After PM 2.5 exposure, the alveolar septum was widened, and the structures of alveolar walls were destroyed. There was inflammatory cells infiltration in the alveolar space and the interstitial space. Alpha diversity in BALF showed that the Chao1, ACE, Simpson, and Shannon values were increased, and the lung microbiome analysis revealed that the relative abundance of Firmicutes and Clostridium increased, while the relative abundance of Bacteroidetes and Akkermansia decreased. The contents of IFN-γ and IL-8 in lung tissue increased while the content of IL-10 decreased. After the administration of AAP, the alveolar structure damage was alleviated, and the interstitial hemorrhage, edema, and inflammatory cells infiltration were reduced. The Chao1 and ACE values decreased, and the taxonomic abundance values of Akkermansia were much higher. Simultaneously, the contents of IFN-γ, IL-4, and IL-8 decreased, and the content of IL-10 increased. Conclusions It was found that PM 2.5 resulted in lung microbiome disorder, which might lead to the inflammation of lung tissue. It was also revealed that AAP could alleviate the inflammatory damage of lung tissue induced by PM 2.5 . Int J Occup Med Environ Health. 2022;35(6):651–64http://ijomeh.eu/Exposure-to-particulate-matter-2-5-leading-to-lung-microbiome-disorder-and-the-alleviation,141469,0,2.htmlpm 2.5inflammatory damagelung injurybalfmicrobiome disorderaap |
| spellingShingle | Yanshu Zhang Bin He Lei Wu Xiaoyi Mi Lijin Zhang Shuang Li Jian Wang Xiaoyu Yu Exposure to particulate matter 2.5 leading to lung microbiome disorder and the alleviation effect of Auricularia auricular-judae polysaccharide International Journal of Occupational Medicine and Environmental Health pm 2.5 inflammatory damage lung injury balf microbiome disorder aap |
| title | Exposure to particulate matter 2.5 leading to lung microbiome disorder and the alleviation effect of Auricularia auricular-judae polysaccharide |
| title_full | Exposure to particulate matter 2.5 leading to lung microbiome disorder and the alleviation effect of Auricularia auricular-judae polysaccharide |
| title_fullStr | Exposure to particulate matter 2.5 leading to lung microbiome disorder and the alleviation effect of Auricularia auricular-judae polysaccharide |
| title_full_unstemmed | Exposure to particulate matter 2.5 leading to lung microbiome disorder and the alleviation effect of Auricularia auricular-judae polysaccharide |
| title_short | Exposure to particulate matter 2.5 leading to lung microbiome disorder and the alleviation effect of Auricularia auricular-judae polysaccharide |
| title_sort | exposure to particulate matter 2 5 leading to lung microbiome disorder and the alleviation effect of auricularia auricular judae polysaccharide |
| topic | pm 2.5 inflammatory damage lung injury balf microbiome disorder aap |
| url | http://ijomeh.eu/Exposure-to-particulate-matter-2-5-leading-to-lung-microbiome-disorder-and-the-alleviation,141469,0,2.html |
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