MAYTENUS MACROCARPA “Chuchuhuasi” decrease the fertility and sperm quality in mice
Introduction: Maytenus macrocarpa (MM) "Chuchuhuasi" is a native plant from the Amazon region of South America, widely used in traditional medicine due its anti-inflammatory, analgesic and anti-tumor properties. However, its effects on reproductive physiology and male fertility have not be...
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| Format: | Article |
| Language: | Spanish |
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Universidad Ricardo Palma
2022-10-01
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| Series: | Revista de la Facultad de Medicina Humana |
| Subjects: | |
| Online Access: | http://revistas.urp.edu.pe/index.php/RFMH/article/view/5115 |
| _version_ | 1797988760602804224 |
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| author | Jonathan Vásquez Cavero Pilar Pino Velásquez José Gonzáles Daga Eduardo Pulido-Murillo José L. Pino Gaviño |
| author_facet | Jonathan Vásquez Cavero Pilar Pino Velásquez José Gonzáles Daga Eduardo Pulido-Murillo José L. Pino Gaviño |
| author_sort | Jonathan Vásquez Cavero |
| collection | DOAJ |
| description | Introduction: Maytenus macrocarpa (MM) "Chuchuhuasi" is a native plant from the Amazon region of South America, widely used in traditional medicine due its anti-inflammatory, analgesic and anti-tumor properties. However, its effects on reproductive physiology and male fertility have not been elucidated.
Methods: Preclinical experimental study of cases and controls. Male mice with eight weeks from birth were administered orally for 70 days (two spermatogenic cycles) an aqueous extract MM: GC (n = 8) 0 mg / kg body weight (bw) control group and treated group GT (n = 8) 1000 mg / kg bw. Later the animals were euthanized, the reproductive organs were weighted, also motility and sperm count were recorded. During the days 60 or 65 of treatment, males were mated and pregnant females were sacrificed in the fourth day of pregnancy for evaluating development, quality and degree of blastulation.
Results: The weights of reproductive organs did not differ significantly between the two groups (p> 0.05). However, progressive motility (32.81 vs. 15.27 ± 4.17% ± 2.08%) and sperm count (7.75 ± 0.34 x 106 / ml vs. 2.56 ± 0.42 x 106 / ml) decreased significantly between GC and GT, respectively. 87.5% (7/8) of the pregnant mice produced 15 litters (GC) and only 50% (4/8) of the pregnant GT yields 4 litters. However, no significant differences were found in the percentage of blastocyst stage embryos, good quality embryos, or embryos with greater than 50% the size of the embryo between the two groups (p> 0.05) blastoceles.
Conclusion: the aqueous extract of Maytenus macrocarpa (1000 mg/kl) could have possesses anti-reproductive action in male mice. |
| first_indexed | 2024-04-11T08:08:00Z |
| format | Article |
| id | doaj.art-4da25fc5ac394f75a0a59b748494d3a1 |
| institution | Directory Open Access Journal |
| issn | 1814-5469 2308-0531 |
| language | Spanish |
| last_indexed | 2024-04-11T08:08:00Z |
| publishDate | 2022-10-01 |
| publisher | Universidad Ricardo Palma |
| record_format | Article |
| series | Revista de la Facultad de Medicina Humana |
| spelling | doaj.art-4da25fc5ac394f75a0a59b748494d3a12022-12-22T04:35:27ZspaUniversidad Ricardo PalmaRevista de la Facultad de Medicina Humana1814-54692308-05312022-10-01224725734https://doi.org/10.25176/RFMH.v22i4.5115MAYTENUS MACROCARPA “Chuchuhuasi” decrease the fertility and sperm quality in miceJonathan Vásquez Cavero0Pilar Pino Velásquez1https://orcid.org/0000-0002-7231-811XJosé Gonzáles Daga2https://orcid.org/0000-0002-3668-5369Eduardo Pulido-Murillo3José L. Pino Gaviño4https://orcid.org/0000-0001-7675-5715Laboratorio de Reproducción y Biología del Desarrollo. Facultad de Ciencias Biológicas. Universidad Nacional Mayor de San Marcos. Lima-Perú.Laboratorio de Reproducción y Biología del Desarrollo. Facultad de Ciencias Biológicas. Universidad Nacional Mayor de San Marcos. Lima-Perú.Laboratorio de Reproducción y Biología del Desarrollo. Facultad de Ciencias Biológicas. Universidad Nacional Mayor de San Marcos. Lima-Perú.Instituto de investigaciones en ciencias biomédicas. Facultad de medicina humana. Universidad Ricardo Palma. Lima, PerúLaboratorio de Reproducción y Biología del Desarrollo. Facultad de Ciencias Biológicas. Universidad Nacional Mayor de San Marcos. Lima-Perú.Introduction: Maytenus macrocarpa (MM) "Chuchuhuasi" is a native plant from the Amazon region of South America, widely used in traditional medicine due its anti-inflammatory, analgesic and anti-tumor properties. However, its effects on reproductive physiology and male fertility have not been elucidated. Methods: Preclinical experimental study of cases and controls. Male mice with eight weeks from birth were administered orally for 70 days (two spermatogenic cycles) an aqueous extract MM: GC (n = 8) 0 mg / kg body weight (bw) control group and treated group GT (n = 8) 1000 mg / kg bw. Later the animals were euthanized, the reproductive organs were weighted, also motility and sperm count were recorded. During the days 60 or 65 of treatment, males were mated and pregnant females were sacrificed in the fourth day of pregnancy for evaluating development, quality and degree of blastulation. Results: The weights of reproductive organs did not differ significantly between the two groups (p> 0.05). However, progressive motility (32.81 vs. 15.27 ± 4.17% ± 2.08%) and sperm count (7.75 ± 0.34 x 106 / ml vs. 2.56 ± 0.42 x 106 / ml) decreased significantly between GC and GT, respectively. 87.5% (7/8) of the pregnant mice produced 15 litters (GC) and only 50% (4/8) of the pregnant GT yields 4 litters. However, no significant differences were found in the percentage of blastocyst stage embryos, good quality embryos, or embryos with greater than 50% the size of the embryo between the two groups (p> 0.05) blastoceles. Conclusion: the aqueous extract of Maytenus macrocarpa (1000 mg/kl) could have possesses anti-reproductive action in male mice.http://revistas.urp.edu.pe/index.php/RFMH/article/view/5115spermatic parametersembryos classificationaqueous extractfertilitymaytenus macrocarpa |
| spellingShingle | Jonathan Vásquez Cavero Pilar Pino Velásquez José Gonzáles Daga Eduardo Pulido-Murillo José L. Pino Gaviño MAYTENUS MACROCARPA “Chuchuhuasi” decrease the fertility and sperm quality in mice Revista de la Facultad de Medicina Humana spermatic parameters embryos classification aqueous extract fertility maytenus macrocarpa |
| title | MAYTENUS MACROCARPA “Chuchuhuasi” decrease the fertility and sperm quality in mice |
| title_full | MAYTENUS MACROCARPA “Chuchuhuasi” decrease the fertility and sperm quality in mice |
| title_fullStr | MAYTENUS MACROCARPA “Chuchuhuasi” decrease the fertility and sperm quality in mice |
| title_full_unstemmed | MAYTENUS MACROCARPA “Chuchuhuasi” decrease the fertility and sperm quality in mice |
| title_short | MAYTENUS MACROCARPA “Chuchuhuasi” decrease the fertility and sperm quality in mice |
| title_sort | maytenus macrocarpa chuchuhuasi decrease the fertility and sperm quality in mice |
| topic | spermatic parameters embryos classification aqueous extract fertility maytenus macrocarpa |
| url | http://revistas.urp.edu.pe/index.php/RFMH/article/view/5115 |
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