Comprehensive Analysis of Autophagy-Related Genes in Rice Immunity against <i>Magnaporthe oryzae</i>
Rice blast disease, caused by the fungus <i>Magnaporthe oryzae</i>, is a significant threat to rice production. Resistant cultivars can effectively resist the invasion of <i>M. oryzae</i>. Thus, the identification of disease-resistant genes is of utmost importance for improvi...
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MDPI AG
2024-03-01
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| author | Xuze Xie Mengtian Pei Shan Liu Xinxiao Wang Shanshan Gong Jing Chen Ye Zhang Zonghua Wang Guodong Lu Ya Li |
| author_facet | Xuze Xie Mengtian Pei Shan Liu Xinxiao Wang Shanshan Gong Jing Chen Ye Zhang Zonghua Wang Guodong Lu Ya Li |
| author_sort | Xuze Xie |
| collection | DOAJ |
| description | Rice blast disease, caused by the fungus <i>Magnaporthe oryzae</i>, is a significant threat to rice production. Resistant cultivars can effectively resist the invasion of <i>M. oryzae</i>. Thus, the identification of disease-resistant genes is of utmost importance for improving rice production. Autophagy, a cellular process that recycles damaged components, plays a vital role in plant growth, development, senescence, stress response, and immunity. To understand the involvement of autophagy-related genes (ATGs) in rice immune response against <i>M. oryzae</i>, we conducted a comprehensive analysis of 37 OsATGs, including bioinformatic analysis, transcriptome analysis, disease resistance analysis, and protein interaction analysis. Bioinformatic analysis revealed that the promoter regions of 33 OsATGs contained cis-acting elements responsive to salicylic acid (SA) or jasmonic acid (JA), two key hormones involved in plant defense responses. Transcriptome data showed that 21 OsATGs were upregulated during <i>M. oryzae</i> infection. Loss-of-function experiments demonstrated that OsATG6c, OsATG8a, OsATG9b, and OsATG13a contribute to rice blast resistance. Additionally, through protein interaction analysis, we identified five proteins that may interact with OsATG13a and potentially contribute to plant immunity. Our study highlights the important role of autophagy in rice immunity and suggests that OsATGs may enhance resistance to rice blast fungus through the involvement of SA, JA, or immune-related proteins. These findings provide valuable insights for future efforts in improving rice production through the identification and utilization of autophagy-related genes. |
| first_indexed | 2024-04-24T10:36:39Z |
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| language | English |
| last_indexed | 2024-04-24T10:36:39Z |
| publishDate | 2024-03-01 |
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| series | Plants |
| spelling | doaj.art-4da2fa03b8f54ebda68ed025e1bf27182024-04-12T13:24:38ZengMDPI AGPlants2223-77472024-03-0113792710.3390/plants13070927Comprehensive Analysis of Autophagy-Related Genes in Rice Immunity against <i>Magnaporthe oryzae</i>Xuze Xie0Mengtian Pei1Shan Liu2Xinxiao Wang3Shanshan Gong4Jing Chen5Ye Zhang6Zonghua Wang7Guodong Lu8Ya Li9State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Universities Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Universities Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Universities Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Universities Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Universities Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Universities Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Universities Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Universities Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Universities Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Universities Key Laboratory for Plant-Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaRice blast disease, caused by the fungus <i>Magnaporthe oryzae</i>, is a significant threat to rice production. Resistant cultivars can effectively resist the invasion of <i>M. oryzae</i>. Thus, the identification of disease-resistant genes is of utmost importance for improving rice production. Autophagy, a cellular process that recycles damaged components, plays a vital role in plant growth, development, senescence, stress response, and immunity. To understand the involvement of autophagy-related genes (ATGs) in rice immune response against <i>M. oryzae</i>, we conducted a comprehensive analysis of 37 OsATGs, including bioinformatic analysis, transcriptome analysis, disease resistance analysis, and protein interaction analysis. Bioinformatic analysis revealed that the promoter regions of 33 OsATGs contained cis-acting elements responsive to salicylic acid (SA) or jasmonic acid (JA), two key hormones involved in plant defense responses. Transcriptome data showed that 21 OsATGs were upregulated during <i>M. oryzae</i> infection. Loss-of-function experiments demonstrated that OsATG6c, OsATG8a, OsATG9b, and OsATG13a contribute to rice blast resistance. Additionally, through protein interaction analysis, we identified five proteins that may interact with OsATG13a and potentially contribute to plant immunity. Our study highlights the important role of autophagy in rice immunity and suggests that OsATGs may enhance resistance to rice blast fungus through the involvement of SA, JA, or immune-related proteins. These findings provide valuable insights for future efforts in improving rice production through the identification and utilization of autophagy-related genes.https://www.mdpi.com/2223-7747/13/7/927autophagyOsATGsplant immunitysalicylic acidjasmonic acid<i>Magnaporthe oryzae</i> |
| spellingShingle | Xuze Xie Mengtian Pei Shan Liu Xinxiao Wang Shanshan Gong Jing Chen Ye Zhang Zonghua Wang Guodong Lu Ya Li Comprehensive Analysis of Autophagy-Related Genes in Rice Immunity against <i>Magnaporthe oryzae</i> Plants autophagy OsATGs plant immunity salicylic acid jasmonic acid <i>Magnaporthe oryzae</i> |
| title | Comprehensive Analysis of Autophagy-Related Genes in Rice Immunity against <i>Magnaporthe oryzae</i> |
| title_full | Comprehensive Analysis of Autophagy-Related Genes in Rice Immunity against <i>Magnaporthe oryzae</i> |
| title_fullStr | Comprehensive Analysis of Autophagy-Related Genes in Rice Immunity against <i>Magnaporthe oryzae</i> |
| title_full_unstemmed | Comprehensive Analysis of Autophagy-Related Genes in Rice Immunity against <i>Magnaporthe oryzae</i> |
| title_short | Comprehensive Analysis of Autophagy-Related Genes in Rice Immunity against <i>Magnaporthe oryzae</i> |
| title_sort | comprehensive analysis of autophagy related genes in rice immunity against i magnaporthe oryzae i |
| topic | autophagy OsATGs plant immunity salicylic acid jasmonic acid <i>Magnaporthe oryzae</i> |
| url | https://www.mdpi.com/2223-7747/13/7/927 |
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