Piceatannol markedly upregulates heme oxygenase-1 expression and alleviates oxidative stress in skeletal muscle cells
Piceatannol (PIC), a phytochemical, is abundant in passion fruit (Passiflora edulis) seeds. In this study, we investigated the effects of PIC on the expression levels of antioxidant enzymes in C2C12 skeletal muscle cells and compared its effects with those of PIC analogues and polyphenols. We also e...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-07-01
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| Series: | Biochemistry and Biophysics Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580819300251 |
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| author | Shiori Nonaka Shinpei Kawakami Hiroko Maruki-Uchida Sadao Mori Minoru Morita |
| author_facet | Shiori Nonaka Shinpei Kawakami Hiroko Maruki-Uchida Sadao Mori Minoru Morita |
| author_sort | Shiori Nonaka |
| collection | DOAJ |
| description | Piceatannol (PIC), a phytochemical, is abundant in passion fruit (Passiflora edulis) seeds. In this study, we investigated the effects of PIC on the expression levels of antioxidant enzymes in C2C12 skeletal muscle cells and compared its effects with those of PIC analogues and polyphenols. We also evaluated its effects on hydrogen peroxide–induced accumulation of reactive oxygen species in C2C12 myotubes. Treatment with PIC led to dose-dependent upregulation of heme oxygenase-1 (Ho-1) and superoxide dismutase 1 (Sod1) mRNA expression in C2C12 myotubes. PIC was the most potent inducer of Ho-1 among the PIC analogues and major polyphenols tested. In addition, treatment with PIC suppressed the hydrogen peroxide–induced increase in intracellular reactive oxygen species levels. Our results suggest that PIC protects skeletal muscles from oxidative stress by activating antioxidant enzymes such as HO-1 and SOD1 and can therefore help prevent oxidative stress–induced muscle dysfunction such as muscle fatigue and sarcopenia. Keywords: Piceatannol, Skeletal muscle cell, HO-1, Oxidative stress, Antioxidant enzymes |
| first_indexed | 2024-04-12T21:21:20Z |
| format | Article |
| id | doaj.art-4da390aae9dc48afaccae1873e24c321 |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-04-12T21:21:20Z |
| publishDate | 2019-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-4da390aae9dc48afaccae1873e24c3212022-12-22T03:16:17ZengElsevierBiochemistry and Biophysics Reports2405-58082019-07-0118Piceatannol markedly upregulates heme oxygenase-1 expression and alleviates oxidative stress in skeletal muscle cellsShiori Nonaka0Shinpei Kawakami1Hiroko Maruki-Uchida2Sadao Mori3Minoru Morita4Research and Development Institute, Health Science Research Center, Morinaga & Co., Ltd., 2-1-1 Shimosueyoshi, Tsurumi-ku, Yokohama, Kanagawa, 230-8504, JapanCorresponding author.; Research and Development Institute, Health Science Research Center, Morinaga & Co., Ltd., 2-1-1 Shimosueyoshi, Tsurumi-ku, Yokohama, Kanagawa, 230-8504, JapanResearch and Development Institute, Health Science Research Center, Morinaga & Co., Ltd., 2-1-1 Shimosueyoshi, Tsurumi-ku, Yokohama, Kanagawa, 230-8504, JapanResearch and Development Institute, Health Science Research Center, Morinaga & Co., Ltd., 2-1-1 Shimosueyoshi, Tsurumi-ku, Yokohama, Kanagawa, 230-8504, JapanResearch and Development Institute, Health Science Research Center, Morinaga & Co., Ltd., 2-1-1 Shimosueyoshi, Tsurumi-ku, Yokohama, Kanagawa, 230-8504, JapanPiceatannol (PIC), a phytochemical, is abundant in passion fruit (Passiflora edulis) seeds. In this study, we investigated the effects of PIC on the expression levels of antioxidant enzymes in C2C12 skeletal muscle cells and compared its effects with those of PIC analogues and polyphenols. We also evaluated its effects on hydrogen peroxide–induced accumulation of reactive oxygen species in C2C12 myotubes. Treatment with PIC led to dose-dependent upregulation of heme oxygenase-1 (Ho-1) and superoxide dismutase 1 (Sod1) mRNA expression in C2C12 myotubes. PIC was the most potent inducer of Ho-1 among the PIC analogues and major polyphenols tested. In addition, treatment with PIC suppressed the hydrogen peroxide–induced increase in intracellular reactive oxygen species levels. Our results suggest that PIC protects skeletal muscles from oxidative stress by activating antioxidant enzymes such as HO-1 and SOD1 and can therefore help prevent oxidative stress–induced muscle dysfunction such as muscle fatigue and sarcopenia. Keywords: Piceatannol, Skeletal muscle cell, HO-1, Oxidative stress, Antioxidant enzymeshttp://www.sciencedirect.com/science/article/pii/S2405580819300251 |
| spellingShingle | Shiori Nonaka Shinpei Kawakami Hiroko Maruki-Uchida Sadao Mori Minoru Morita Piceatannol markedly upregulates heme oxygenase-1 expression and alleviates oxidative stress in skeletal muscle cells Biochemistry and Biophysics Reports |
| title | Piceatannol markedly upregulates heme oxygenase-1 expression and alleviates oxidative stress in skeletal muscle cells |
| title_full | Piceatannol markedly upregulates heme oxygenase-1 expression and alleviates oxidative stress in skeletal muscle cells |
| title_fullStr | Piceatannol markedly upregulates heme oxygenase-1 expression and alleviates oxidative stress in skeletal muscle cells |
| title_full_unstemmed | Piceatannol markedly upregulates heme oxygenase-1 expression and alleviates oxidative stress in skeletal muscle cells |
| title_short | Piceatannol markedly upregulates heme oxygenase-1 expression and alleviates oxidative stress in skeletal muscle cells |
| title_sort | piceatannol markedly upregulates heme oxygenase 1 expression and alleviates oxidative stress in skeletal muscle cells |
| url | http://www.sciencedirect.com/science/article/pii/S2405580819300251 |
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