Genomic, phenotypic and demographic characterization of Mycobacterium tuberculosis in Israel in 2021
According to World Health Organization WHO, Tuberculosis (TB) is the second cause of death from infectious disease worldwide. During 2021, 10.6 million people were infected with TB, and 1.6 million people died. TB is caused by pathogens belonging to the Mycobacterium tuberculosis complex (MTBC), mai...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1196904/full |
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| author | Yelena Losev Mor Rubinstein Israel Nissan Paz Haviv Yohi Barsky Martha Volinsky Gefen Bar-Giora Tamara Zouher Mazal Hamawi Gal Zizelski Valenci Ina Kutikov Hasia Kaidar Shwartz Zeev Dveyrin Daniel Chemtob Daniel Chemtob Efrat Rorman |
| author_facet | Yelena Losev Mor Rubinstein Israel Nissan Paz Haviv Yohi Barsky Martha Volinsky Gefen Bar-Giora Tamara Zouher Mazal Hamawi Gal Zizelski Valenci Ina Kutikov Hasia Kaidar Shwartz Zeev Dveyrin Daniel Chemtob Daniel Chemtob Efrat Rorman |
| author_sort | Yelena Losev |
| collection | DOAJ |
| description | According to World Health Organization WHO, Tuberculosis (TB) is the second cause of death from infectious disease worldwide. During 2021, 10.6 million people were infected with TB, and 1.6 million people died. TB is caused by pathogens belonging to the Mycobacterium tuberculosis complex (MTBC), mainly by Mycobacterium tuberculosis (M.tb). Members of this complex are acid-fast bacilli, which can cause intrapulmonary and extra pulmonary TB, and can be divided into various lineages, based on genomic markers. The main public health threat comes from drug resistant M.tb strains, which are responsible for about 25% of TB death and treatment failure worldwide. Treating drug resistant TB patients significantly raises the costs of TB treatment. This study provides an overview of the demographic and drug susceptibility characteristics of newly diagnosed TB patients in Israel in 2021. The State of Israel has a very low level of TB endemicity and is at a pre-elimination phase. Notably, only 11.7% of the newly diagnosed TB patients were born in Israel. In this report, of the 154 new laboratory-confirmed TB patients, 66.7% had pulmonary TB, while 16% had extrapulmonary TB. Males accounted for 52% of the patients, with the most prevalent age group being 21-40. Most patients were citizens of Israel (53.9%), while 37.7% had no Israeli citizenship. Among non-citizens, there was a predominance of males and patients aged 21-40. The susceptibility profile showed a high resistance rate to streptomycin (18.2%) and to a lower extent to isoniazid (13.6%), pyrazinamide (8.4%), rifampicin (7.8%), and ethambutol (3.2%). Only 2 cases of XDR-TB and 10 MDR-TB strains were detected in Israel in 2021, with both XDR strains and 5 out of 10 MDR strains belonging to the Beijing lineage. Most of Beijing isolates were resistant to at least one tested drug. Genomic sequencing of 134 out of 156 strains and bioinformatics analysis using the MTBseq program and WHO mutation catalogue shows a good match with only 9 discrepancies between phenotypic and genotypic susceptibility profiles in first line drugs. The most common lineage is Delhi-Cas (23%) followed by the Beijing lineage (17%). Most patients from the Delhi-Cas lineage were born in Africa, while patients with Beijing isolates were born in different countries. Minimum spanning tree analysis identified 15 clusters. The study highlights the need for ongoing surveillance of TB using molecular and phenotypic tools to further decreasing the spreading level of the disease and develop effective treatment strategies. |
| first_indexed | 2024-03-11T17:37:43Z |
| format | Article |
| id | doaj.art-4dac0cd26cbf4563b9ccee306aebaea6 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-03-11T17:37:43Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-4dac0cd26cbf4563b9ccee306aebaea62023-10-18T18:05:16ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-10-011310.3389/fcimb.2023.11969041196904Genomic, phenotypic and demographic characterization of Mycobacterium tuberculosis in Israel in 2021Yelena Losev0Mor Rubinstein1Israel Nissan2Paz Haviv3Yohi Barsky4Martha Volinsky5Gefen Bar-Giora6Tamara Zouher7Mazal Hamawi8Gal Zizelski Valenci9Ina Kutikov10Hasia Kaidar Shwartz11Zeev Dveyrin12Daniel Chemtob13Daniel Chemtob14Efrat Rorman15National Public Health Laboratory, Public Health Directorate, Ministry of Health, Tel Aviv, IsraelNational Public Health Laboratory, Public Health Directorate, Ministry of Health, Tel Aviv, IsraelNational Public Health Laboratory, Public Health Directorate, Ministry of Health, Tel Aviv, IsraelNational Public Health Laboratory, Public Health Directorate, Ministry of Health, Tel Aviv, IsraelNational Public Health Laboratory, Public Health Directorate, Ministry of Health, Tel Aviv, IsraelNational Public Health Laboratory, Public Health Directorate, Ministry of Health, Tel Aviv, IsraelNational Public Health Laboratory, Public Health Directorate, Ministry of Health, Tel Aviv, IsraelNational Public Health Laboratory, Public Health Directorate, Ministry of Health, Tel Aviv, IsraelNational Public Health Laboratory, Public Health Directorate, Ministry of Health, Tel Aviv, IsraelNational Public Health Laboratory, Public Health Directorate, Ministry of Health, Tel Aviv, IsraelNational Public Health Laboratory, Public Health Directorate, Ministry of Health, Tel Aviv, IsraelNational Public Health Laboratory, Public Health Directorate, Ministry of Health, Tel Aviv, IsraelNational Public Health Laboratory, Public Health Directorate, Ministry of Health, Tel Aviv, IsraelDepartment of Tuberculosis (TB) and AIDS and National TB Program Manager, Ministry of Health, Jerusalem, IsraelHebrew University-Hadassah Faculty of Medicine, School of Public Health and Community Medicine, Jerusalem, IsraelNational Public Health Laboratory, Public Health Directorate, Ministry of Health, Tel Aviv, IsraelAccording to World Health Organization WHO, Tuberculosis (TB) is the second cause of death from infectious disease worldwide. During 2021, 10.6 million people were infected with TB, and 1.6 million people died. TB is caused by pathogens belonging to the Mycobacterium tuberculosis complex (MTBC), mainly by Mycobacterium tuberculosis (M.tb). Members of this complex are acid-fast bacilli, which can cause intrapulmonary and extra pulmonary TB, and can be divided into various lineages, based on genomic markers. The main public health threat comes from drug resistant M.tb strains, which are responsible for about 25% of TB death and treatment failure worldwide. Treating drug resistant TB patients significantly raises the costs of TB treatment. This study provides an overview of the demographic and drug susceptibility characteristics of newly diagnosed TB patients in Israel in 2021. The State of Israel has a very low level of TB endemicity and is at a pre-elimination phase. Notably, only 11.7% of the newly diagnosed TB patients were born in Israel. In this report, of the 154 new laboratory-confirmed TB patients, 66.7% had pulmonary TB, while 16% had extrapulmonary TB. Males accounted for 52% of the patients, with the most prevalent age group being 21-40. Most patients were citizens of Israel (53.9%), while 37.7% had no Israeli citizenship. Among non-citizens, there was a predominance of males and patients aged 21-40. The susceptibility profile showed a high resistance rate to streptomycin (18.2%) and to a lower extent to isoniazid (13.6%), pyrazinamide (8.4%), rifampicin (7.8%), and ethambutol (3.2%). Only 2 cases of XDR-TB and 10 MDR-TB strains were detected in Israel in 2021, with both XDR strains and 5 out of 10 MDR strains belonging to the Beijing lineage. Most of Beijing isolates were resistant to at least one tested drug. Genomic sequencing of 134 out of 156 strains and bioinformatics analysis using the MTBseq program and WHO mutation catalogue shows a good match with only 9 discrepancies between phenotypic and genotypic susceptibility profiles in first line drugs. The most common lineage is Delhi-Cas (23%) followed by the Beijing lineage (17%). Most patients from the Delhi-Cas lineage were born in Africa, while patients with Beijing isolates were born in different countries. Minimum spanning tree analysis identified 15 clusters. The study highlights the need for ongoing surveillance of TB using molecular and phenotypic tools to further decreasing the spreading level of the disease and develop effective treatment strategies.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1196904/fullMycobacterium tuberculosis complex (MTBC)next generation sequencing (NGS)whole genome sequencing (WGS)Israelmigrantsmultidrug resistant M.tb (MDR-TB) |
| spellingShingle | Yelena Losev Mor Rubinstein Israel Nissan Paz Haviv Yohi Barsky Martha Volinsky Gefen Bar-Giora Tamara Zouher Mazal Hamawi Gal Zizelski Valenci Ina Kutikov Hasia Kaidar Shwartz Zeev Dveyrin Daniel Chemtob Daniel Chemtob Efrat Rorman Genomic, phenotypic and demographic characterization of Mycobacterium tuberculosis in Israel in 2021 Frontiers in Cellular and Infection Microbiology Mycobacterium tuberculosis complex (MTBC) next generation sequencing (NGS) whole genome sequencing (WGS) Israel migrants multidrug resistant M.tb (MDR-TB) |
| title | Genomic, phenotypic and demographic characterization of Mycobacterium tuberculosis in Israel in 2021 |
| title_full | Genomic, phenotypic and demographic characterization of Mycobacterium tuberculosis in Israel in 2021 |
| title_fullStr | Genomic, phenotypic and demographic characterization of Mycobacterium tuberculosis in Israel in 2021 |
| title_full_unstemmed | Genomic, phenotypic and demographic characterization of Mycobacterium tuberculosis in Israel in 2021 |
| title_short | Genomic, phenotypic and demographic characterization of Mycobacterium tuberculosis in Israel in 2021 |
| title_sort | genomic phenotypic and demographic characterization of mycobacterium tuberculosis in israel in 2021 |
| topic | Mycobacterium tuberculosis complex (MTBC) next generation sequencing (NGS) whole genome sequencing (WGS) Israel migrants multidrug resistant M.tb (MDR-TB) |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1196904/full |
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