Spatio-temporal activation patterns of neuronal population evoked by optostimulation and the comparison to electrical microstimulation
Abstract Optostimulation and electrical microstimulation are well-established techniques that enable to artificially stimulate the brain. While the activation patterns evoked by microstimulation in cortical network are well characterized, much less is known for optostimulation. Specifically, the act...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-39808-w |
| _version_ | 1797452713646096384 |
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| author | Shany Nivinsky Margalit Hamutal Slovin |
| author_facet | Shany Nivinsky Margalit Hamutal Slovin |
| author_sort | Shany Nivinsky Margalit |
| collection | DOAJ |
| description | Abstract Optostimulation and electrical microstimulation are well-established techniques that enable to artificially stimulate the brain. While the activation patterns evoked by microstimulation in cortical network are well characterized, much less is known for optostimulation. Specifically, the activation maps of neuronal population at the membrane potential level and direct measurements of these maps were barely reported. In addition, only a few studies compared the activation patterns evoked by microstimulation and optostimulation. In this study we addressed these issues by applying optostimulation in the barrel cortex of anesthetized rats after a short (ShortExp) or a long (LongExp) opsin expression time and compared it to microstimulation. We measured the membrane potential of neuronal populations at high spatial (meso-scale) and temporal resolution using voltage-sensitive dye imaging. Longer optostimulation pulses evoked higher neural responses spreading over larger region relative to short pulses. Interestingly, similar optostimulation pulses evoked stronger and more prolonged population response in the LongExp vs. the ShortExp condition. Finally, the spatial activation patterns evoked in the LongExp condition showed an intermediate state, with higher resemblance to the microstimulation at the stimulation site. Therefore, short microstimulation and optostimulation can induce wide spread activation, however the effects of optostimulation depend on the opsin expression time. |
| first_indexed | 2024-03-09T15:12:38Z |
| format | Article |
| id | doaj.art-4dadc8ee1a094adfb4f7a41e8f29ddd3 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:12:38Z |
| publishDate | 2023-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-4dadc8ee1a094adfb4f7a41e8f29ddd32023-11-26T13:16:22ZengNature PortfolioScientific Reports2045-23222023-08-0113111410.1038/s41598-023-39808-wSpatio-temporal activation patterns of neuronal population evoked by optostimulation and the comparison to electrical microstimulationShany Nivinsky Margalit0Hamutal Slovin1The Gonda Multidisciplinary Brain Research Center, Bar-Ilan UniversityThe Gonda Multidisciplinary Brain Research Center, Bar-Ilan UniversityAbstract Optostimulation and electrical microstimulation are well-established techniques that enable to artificially stimulate the brain. While the activation patterns evoked by microstimulation in cortical network are well characterized, much less is known for optostimulation. Specifically, the activation maps of neuronal population at the membrane potential level and direct measurements of these maps were barely reported. In addition, only a few studies compared the activation patterns evoked by microstimulation and optostimulation. In this study we addressed these issues by applying optostimulation in the barrel cortex of anesthetized rats after a short (ShortExp) or a long (LongExp) opsin expression time and compared it to microstimulation. We measured the membrane potential of neuronal populations at high spatial (meso-scale) and temporal resolution using voltage-sensitive dye imaging. Longer optostimulation pulses evoked higher neural responses spreading over larger region relative to short pulses. Interestingly, similar optostimulation pulses evoked stronger and more prolonged population response in the LongExp vs. the ShortExp condition. Finally, the spatial activation patterns evoked in the LongExp condition showed an intermediate state, with higher resemblance to the microstimulation at the stimulation site. Therefore, short microstimulation and optostimulation can induce wide spread activation, however the effects of optostimulation depend on the opsin expression time.https://doi.org/10.1038/s41598-023-39808-w |
| spellingShingle | Shany Nivinsky Margalit Hamutal Slovin Spatio-temporal activation patterns of neuronal population evoked by optostimulation and the comparison to electrical microstimulation Scientific Reports |
| title | Spatio-temporal activation patterns of neuronal population evoked by optostimulation and the comparison to electrical microstimulation |
| title_full | Spatio-temporal activation patterns of neuronal population evoked by optostimulation and the comparison to electrical microstimulation |
| title_fullStr | Spatio-temporal activation patterns of neuronal population evoked by optostimulation and the comparison to electrical microstimulation |
| title_full_unstemmed | Spatio-temporal activation patterns of neuronal population evoked by optostimulation and the comparison to electrical microstimulation |
| title_short | Spatio-temporal activation patterns of neuronal population evoked by optostimulation and the comparison to electrical microstimulation |
| title_sort | spatio temporal activation patterns of neuronal population evoked by optostimulation and the comparison to electrical microstimulation |
| url | https://doi.org/10.1038/s41598-023-39808-w |
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