Naringin ameliorates the high glucose-induced rat mesangial cell inflammatory reaction by modulating the NLRP3 Inflammasome
Abstract Background The Nucleotide binding and oligomerization domain-like receptorfamily pyrin domain-containing 3 (NLRP3)-inflammasome plays an important role in various diseases, including a variety of kidney diseases. Naringin exhibits anti-inflammatory and anti-oxidation effects among others, b...
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| Format: | Article |
| Language: | English |
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BMC
2018-06-01
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| Series: | BMC Complementary and Alternative Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s12906-018-2257-y |
| _version_ | 1818061593876889600 |
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| author | Fenqin Chen Guozhu Wei Jiao Xu Xiaoyu Ma Qiuyue Wang |
| author_facet | Fenqin Chen Guozhu Wei Jiao Xu Xiaoyu Ma Qiuyue Wang |
| author_sort | Fenqin Chen |
| collection | DOAJ |
| description | Abstract Background The Nucleotide binding and oligomerization domain-like receptorfamily pyrin domain-containing 3 (NLRP3)-inflammasome plays an important role in various diseases, including a variety of kidney diseases. Naringin exhibits anti-inflammatory and anti-oxidation effects among others, but its specific mechanisms are not clear. We investigated the expression of the NLRP3-inflammasome under high-glucose conditions, assessed the effects of naringin on that process, and further elucidated the role of naringin in the pathogenesis of diabetic kidney disease(DKD). Methods To assess the therapeutic potential of naringin and the mechanisms involved, we cultured rat glomerular mesangial cells and grouped them according to different glucose concentrations, different action times, different concentrations of MCC950, and different concentrations of naringin.The cell proliferation was measured by MTT assay. The expression of Interleukin-1β(IL-1β) and Interleukin18 (IL-18) in the cell supernatant were detected by ELISA. The expression and activity of NLPR3, apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) and Caspase-1 were detected by Western Blot. Results The expressions of NLRP3, ASC, caspase-1, IL-1β, and IL-18 in rat glomerular mesangial cells were significantly higher in the high glucose (HG) group than in the control normal glucose (NG) group and exhibited time-dependence activity. The expression levels of NLRP3, caspase-1, IL-1β, and IL-18 in different treatment groups were significantly lower compared with the HG group after 48 h of MCC950 pre-treatment (p < 0.05). Pre-treatment with naringin produced the same results. Naringin also inhibited the proliferation of cells. Conclusions The NLRP3-inflammasome potentially plays a role in the process of activation and inflammation of glomerular mesangial cells as induced by high-glucose conditions. Naringin inhibited the proliferation of cells that were induced by high glucose. Further, it reduced the expression of inflammatory factors that are mediated by NLRP3 through the NLRP3-caspase-1-IL-1β/IL-18 signaling pathway, which makes naringin a potentially novel treatment for DKD disease. |
| first_indexed | 2024-12-10T13:50:47Z |
| format | Article |
| id | doaj.art-4daf7c7daad64658858da95e128a4657 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-10T13:50:47Z |
| publishDate | 2018-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-4daf7c7daad64658858da95e128a46572022-12-22T01:46:11ZengBMCBMC Complementary and Alternative Medicine1472-68822018-06-0118111110.1186/s12906-018-2257-yNaringin ameliorates the high glucose-induced rat mesangial cell inflammatory reaction by modulating the NLRP3 InflammasomeFenqin Chen0Guozhu Wei1Jiao Xu2Xiaoyu Ma3Qiuyue Wang4Departments of Geriatric, the First Affiliated Hospital, China Medical UniversityDepartment of Radiology, Orthopedic Hospital of ShenyangDepartments of Geriatric, the First Affiliated Hospital, China Medical UniversityDepartments of Geriatric, the First Affiliated Hospital, China Medical UniversityDepartments of Endocrinology, the First Affiliated Hospital, China Medical UniversityAbstract Background The Nucleotide binding and oligomerization domain-like receptorfamily pyrin domain-containing 3 (NLRP3)-inflammasome plays an important role in various diseases, including a variety of kidney diseases. Naringin exhibits anti-inflammatory and anti-oxidation effects among others, but its specific mechanisms are not clear. We investigated the expression of the NLRP3-inflammasome under high-glucose conditions, assessed the effects of naringin on that process, and further elucidated the role of naringin in the pathogenesis of diabetic kidney disease(DKD). Methods To assess the therapeutic potential of naringin and the mechanisms involved, we cultured rat glomerular mesangial cells and grouped them according to different glucose concentrations, different action times, different concentrations of MCC950, and different concentrations of naringin.The cell proliferation was measured by MTT assay. The expression of Interleukin-1β(IL-1β) and Interleukin18 (IL-18) in the cell supernatant were detected by ELISA. The expression and activity of NLPR3, apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) and Caspase-1 were detected by Western Blot. Results The expressions of NLRP3, ASC, caspase-1, IL-1β, and IL-18 in rat glomerular mesangial cells were significantly higher in the high glucose (HG) group than in the control normal glucose (NG) group and exhibited time-dependence activity. The expression levels of NLRP3, caspase-1, IL-1β, and IL-18 in different treatment groups were significantly lower compared with the HG group after 48 h of MCC950 pre-treatment (p < 0.05). Pre-treatment with naringin produced the same results. Naringin also inhibited the proliferation of cells. Conclusions The NLRP3-inflammasome potentially plays a role in the process of activation and inflammation of glomerular mesangial cells as induced by high-glucose conditions. Naringin inhibited the proliferation of cells that were induced by high glucose. Further, it reduced the expression of inflammatory factors that are mediated by NLRP3 through the NLRP3-caspase-1-IL-1β/IL-18 signaling pathway, which makes naringin a potentially novel treatment for DKD disease.http://link.springer.com/article/10.1186/s12906-018-2257-yDiabetes mellitusDiabetic kidney diseaseNLRP3 inflammasomeNLRP3-caspase-1-IL-1β/IL-18 signaling pathwayNaringin |
| spellingShingle | Fenqin Chen Guozhu Wei Jiao Xu Xiaoyu Ma Qiuyue Wang Naringin ameliorates the high glucose-induced rat mesangial cell inflammatory reaction by modulating the NLRP3 Inflammasome BMC Complementary and Alternative Medicine Diabetes mellitus Diabetic kidney disease NLRP3 inflammasome NLRP3-caspase-1-IL-1β/IL-18 signaling pathway Naringin |
| title | Naringin ameliorates the high glucose-induced rat mesangial cell inflammatory reaction by modulating the NLRP3 Inflammasome |
| title_full | Naringin ameliorates the high glucose-induced rat mesangial cell inflammatory reaction by modulating the NLRP3 Inflammasome |
| title_fullStr | Naringin ameliorates the high glucose-induced rat mesangial cell inflammatory reaction by modulating the NLRP3 Inflammasome |
| title_full_unstemmed | Naringin ameliorates the high glucose-induced rat mesangial cell inflammatory reaction by modulating the NLRP3 Inflammasome |
| title_short | Naringin ameliorates the high glucose-induced rat mesangial cell inflammatory reaction by modulating the NLRP3 Inflammasome |
| title_sort | naringin ameliorates the high glucose induced rat mesangial cell inflammatory reaction by modulating the nlrp3 inflammasome |
| topic | Diabetes mellitus Diabetic kidney disease NLRP3 inflammasome NLRP3-caspase-1-IL-1β/IL-18 signaling pathway Naringin |
| url | http://link.springer.com/article/10.1186/s12906-018-2257-y |
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