Amelioration of pain and anxiety in sleep-deprived rats by intra-amygdala injection of cinnamaldehyde
Background: Sleep disorders are accompanied by increased anxiety and somatic pain. In addition, it has been observed that anxiety and pain have a boosting effect on each other, resulting in continued sleep disturbances. Amygdala's (CeA) central nucleus plays a crucial role in these processes. C...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Sleep Medicine: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590142723000095 |
| _version_ | 1827919371579686912 |
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| author | Seyed Kaveh Hadeiy Solomon Habtemariam Zeinab Shankayi Shima Shahyad Hedayat Sahraei Milad Asghardoust Rezaei Farideh Bahrami |
| author_facet | Seyed Kaveh Hadeiy Solomon Habtemariam Zeinab Shankayi Shima Shahyad Hedayat Sahraei Milad Asghardoust Rezaei Farideh Bahrami |
| author_sort | Seyed Kaveh Hadeiy |
| collection | DOAJ |
| description | Background: Sleep disorders are accompanied by increased anxiety and somatic pain. In addition, it has been observed that anxiety and pain have a boosting effect on each other, resulting in continued sleep disturbances. Amygdala's (CeA) central nucleus plays a crucial role in these processes. Cinnamaldehyde (Cinn) is an aromatic compound with anti-anxiety, antioxidant, and sleep-promoting properties. The present study uses sleep-deprived rats to examine the effects of an intra-CeA injection of Cinn on pain and anxiety. Methods: Sleep deprivation (SD) was induced using the platform technique. 35 male Wistar rats were divided into five groups. Anxiety state and nociception were evaluated among groups using formalin test (F.T.), open field test (OFT), and elevated plus maze (EPM). Anxiety tests (OFT and EPM) were conducted in all groups. The first group was undergone FT without induction of SD (SD−FT+). The second group received SD without FT(SD+FT−). The third group received both SD and FT(SD+FT+). The treatment and vehicle groups have undergone both SD and FT in addition to the respectively intra-CeA injection of Cinn (SD+FT+ Cinn) and Cinn vehicle (SD+FT+ VC). The recorded behaviors were analyzed between groups using IBM SPSS 24th version. Results: SD did not lead to any significant difference in nociceptive behaviors in FT between groups SD−FT+ and SD+FT+ (P ≥ 0.05). At the same time, there was a considerable discrepancy in rearing behaviors (P < 0.006) and the number of fecal boli (P < 0.004) recorded in OFM between these groups. Treatment with Cinn led to decreased nociception (P < 0.038), decreased rearing behaviors (P < 0.01), and reduced defecation (P < 0.004) in group SD + FT+ Cinn in comparison to the group SD+FT+. There were no differences in anxiety test results between the first and second groups (P ≥ 0.05). Conclusion: SD can lead to elevated anxiety, while intra-CeA injection of Cinn ameliorated both perceptions of acute pain and anxiety. Besides, the conduction of FT before the anxiety test led to no disturbance in the results of anxiety tests. |
| first_indexed | 2024-03-13T03:56:52Z |
| format | Article |
| id | doaj.art-4db0f1b5d81a459c879eab49988b0ecb |
| institution | Directory Open Access Journal |
| issn | 2590-1427 |
| language | English |
| last_indexed | 2024-03-13T03:56:52Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sleep Medicine: X |
| spelling | doaj.art-4db0f1b5d81a459c879eab49988b0ecb2023-06-22T05:04:42ZengElsevierSleep Medicine: X2590-14272023-12-015100069Amelioration of pain and anxiety in sleep-deprived rats by intra-amygdala injection of cinnamaldehydeSeyed Kaveh Hadeiy0Solomon Habtemariam1Zeinab Shankayi2Shima Shahyad3Hedayat Sahraei4Milad Asghardoust Rezaei5Farideh Bahrami6Social Determinants of Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, IranPharmacognosy Research Laboratories & Herbal Analysis Services U.K., University of Greenwich, Chatham-Maritime, Kent, ME4 4 T, UK.B., UKNeuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran; Department of physiology and medical physics, school of medicine, Baqiyatallah University of medical sciences, Tehran, IranNeuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, IranNeuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran; Department of physiology and medical physics, school of medicine, Baqiyatallah University of medical sciences, Tehran, IranStudents' Research Committee, Baqiyatallah University of Medical Sciences, Tehran, IranNeuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran; Department of physiology and medical physics, school of medicine, Baqiyatallah University of medical sciences, Tehran, Iran; Corresponding author. Department of Physiology and Medical Physics, School of Medicine, Baqiyatallah University of Medical Sciences, South Sheikh Bahaei Avenue, Tehran, Iran.Background: Sleep disorders are accompanied by increased anxiety and somatic pain. In addition, it has been observed that anxiety and pain have a boosting effect on each other, resulting in continued sleep disturbances. Amygdala's (CeA) central nucleus plays a crucial role in these processes. Cinnamaldehyde (Cinn) is an aromatic compound with anti-anxiety, antioxidant, and sleep-promoting properties. The present study uses sleep-deprived rats to examine the effects of an intra-CeA injection of Cinn on pain and anxiety. Methods: Sleep deprivation (SD) was induced using the platform technique. 35 male Wistar rats were divided into five groups. Anxiety state and nociception were evaluated among groups using formalin test (F.T.), open field test (OFT), and elevated plus maze (EPM). Anxiety tests (OFT and EPM) were conducted in all groups. The first group was undergone FT without induction of SD (SD−FT+). The second group received SD without FT(SD+FT−). The third group received both SD and FT(SD+FT+). The treatment and vehicle groups have undergone both SD and FT in addition to the respectively intra-CeA injection of Cinn (SD+FT+ Cinn) and Cinn vehicle (SD+FT+ VC). The recorded behaviors were analyzed between groups using IBM SPSS 24th version. Results: SD did not lead to any significant difference in nociceptive behaviors in FT between groups SD−FT+ and SD+FT+ (P ≥ 0.05). At the same time, there was a considerable discrepancy in rearing behaviors (P < 0.006) and the number of fecal boli (P < 0.004) recorded in OFM between these groups. Treatment with Cinn led to decreased nociception (P < 0.038), decreased rearing behaviors (P < 0.01), and reduced defecation (P < 0.004) in group SD + FT+ Cinn in comparison to the group SD+FT+. There were no differences in anxiety test results between the first and second groups (P ≥ 0.05). Conclusion: SD can lead to elevated anxiety, while intra-CeA injection of Cinn ameliorated both perceptions of acute pain and anxiety. Besides, the conduction of FT before the anxiety test led to no disturbance in the results of anxiety tests.http://www.sciencedirect.com/science/article/pii/S2590142723000095CinnamaldehydeSleep deprivationAnxietyPainAmygdala |
| spellingShingle | Seyed Kaveh Hadeiy Solomon Habtemariam Zeinab Shankayi Shima Shahyad Hedayat Sahraei Milad Asghardoust Rezaei Farideh Bahrami Amelioration of pain and anxiety in sleep-deprived rats by intra-amygdala injection of cinnamaldehyde Sleep Medicine: X Cinnamaldehyde Sleep deprivation Anxiety Pain Amygdala |
| title | Amelioration of pain and anxiety in sleep-deprived rats by intra-amygdala injection of cinnamaldehyde |
| title_full | Amelioration of pain and anxiety in sleep-deprived rats by intra-amygdala injection of cinnamaldehyde |
| title_fullStr | Amelioration of pain and anxiety in sleep-deprived rats by intra-amygdala injection of cinnamaldehyde |
| title_full_unstemmed | Amelioration of pain and anxiety in sleep-deprived rats by intra-amygdala injection of cinnamaldehyde |
| title_short | Amelioration of pain and anxiety in sleep-deprived rats by intra-amygdala injection of cinnamaldehyde |
| title_sort | amelioration of pain and anxiety in sleep deprived rats by intra amygdala injection of cinnamaldehyde |
| topic | Cinnamaldehyde Sleep deprivation Anxiety Pain Amygdala |
| url | http://www.sciencedirect.com/science/article/pii/S2590142723000095 |
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