Modified Baihu decoction therapeutically remodels gut microbiota to inhibit acute gouty arthritis
Background: Acute gouty arthritis (AGA) is the most common first symptom of gout, and the development of gout as a metabolic and immune inflammatory disease is also correlated with the gut microbiota. However, the mechanism of the effect of changes in the gut microbiota on AGA remains unclear. The i...
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2022.1023453/full |
| _version_ | 1798006385449893888 |
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| author | Xianyang Wang Haishan Long Ming Chen Zongbo Zhou Qinlin Wu Shijie Xu Geng Li Zhifu Lu |
| author_facet | Xianyang Wang Haishan Long Ming Chen Zongbo Zhou Qinlin Wu Shijie Xu Geng Li Zhifu Lu |
| author_sort | Xianyang Wang |
| collection | DOAJ |
| description | Background: Acute gouty arthritis (AGA) is the most common first symptom of gout, and the development of gout as a metabolic and immune inflammatory disease is also correlated with the gut microbiota. However, the mechanism of the effect of changes in the gut microbiota on AGA remains unclear. The intestinal flora can not only affect purine metabolism or regulate inflammation, but also influence the therapeutic effect of drugs on AGA. The aim of this study was to investigate the exact mechanism of modified Baihu decoction (MBD) in the treatment of AGA and whether it is related to the regulation of the structure of the intestinal flora.Methods: On the 21st day of MBD administration by continuous gavage, a rat acute gouty arthritis model was constructed using sodium urate (0.1 mL/rat, 50 mg/mL), and the ankle joint swelling was measured before and 4 h, 8 h, 24 h, and 48 h after the injection of sodium urate. After 48 h of sodium urate injection, serum, liver, kidney, ankle synovial tissue and feces were collected from rats. The collected samples were examined and analyzed using H&E, Elisa, Immunohistochemistry, Histopathology, 16S rDNA, and Biochemical analysis. To investigate the mechanism of MBD to alleviate AGA using pro-inflammatory factors and intestinal flora.Results: MBD (5.84, 35 g/kg) was administered orally to AGA rats and diclofenac sodium tablets (DS-tablets) were used as standard treatment control. Serum biochemical assessment confirmed that MBD is a safe drug for the treatment of AGA. In addition, our findings confirmed that MBD relieved AGA-related symptoms, such as toe swelling. Lowering serum levels of uric acid, IL-1β, and TGF-β1 immunohistochemical results also confirmed that MBD reduced the expression of inflammatory elements such as IL-1β, NLRP3, ASC, and Caspase-1 in synovial tissue.Furthermore, compared with control group, the 16s rDNA sequencing of AGA rat faeces revealed an increase in the relative abundance of Lachnospiraceae, Muribaculaceae, and Bifidobacteriaceae species. While the relative abundance of Lactobacillaceae, Erysipelotrichaceae, Ruminococcaceae, Prevotellaceae and Enterobacteriaceae showed a relative decrease in species abundance. Of these, the reduction in species abundance of Enterobacteriaceae was associated with a reduction in amino acid metabolism and environmental perception. After MBD therapeutic intervention, the disturbance of the intestinal flora caused by AGA was restored.Conclusion: In summary, MBD is an effective agent for the treatment of AGA, with the potential mechanism being the regulation of intestinal flora to control inflammation. This would help to promote the therapeutic effect of MBD on AGA. |
| first_indexed | 2024-04-11T12:55:04Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-04-11T12:55:04Z |
| publishDate | 2022-12-01 |
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| spelling | doaj.art-4db3189596814329bcc64bf365e0b0f72022-12-22T04:23:06ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2022-12-011310.3389/fphys.2022.10234531023453Modified Baihu decoction therapeutically remodels gut microbiota to inhibit acute gouty arthritisXianyang Wang0Haishan Long1Ming Chen2Zongbo Zhou3Qinlin Wu4Shijie Xu5Geng Li6Zhifu Lu7Animal Experiment Center, Guangzhou University of Chinese Medicine, Guangzhou, ChinaAnimal Experiment Center, Guangzhou University of Chinese Medicine, Guangzhou, ChinaHaikou Hospital of Traditional Chinese Medicine, Haikou, Hainan, ChinaHaikou Hospital of Traditional Chinese Medicine, Haikou, Hainan, ChinaHaikou Hospital of Traditional Chinese Medicine, Haikou, Hainan, ChinaGuangzhou University of Chinese Medicine, Guangzhou, ChinaAnimal Experiment Center, Guangzhou University of Chinese Medicine, Guangzhou, ChinaHaikou Hospital of Traditional Chinese Medicine, Haikou, Hainan, ChinaBackground: Acute gouty arthritis (AGA) is the most common first symptom of gout, and the development of gout as a metabolic and immune inflammatory disease is also correlated with the gut microbiota. However, the mechanism of the effect of changes in the gut microbiota on AGA remains unclear. The intestinal flora can not only affect purine metabolism or regulate inflammation, but also influence the therapeutic effect of drugs on AGA. The aim of this study was to investigate the exact mechanism of modified Baihu decoction (MBD) in the treatment of AGA and whether it is related to the regulation of the structure of the intestinal flora.Methods: On the 21st day of MBD administration by continuous gavage, a rat acute gouty arthritis model was constructed using sodium urate (0.1 mL/rat, 50 mg/mL), and the ankle joint swelling was measured before and 4 h, 8 h, 24 h, and 48 h after the injection of sodium urate. After 48 h of sodium urate injection, serum, liver, kidney, ankle synovial tissue and feces were collected from rats. The collected samples were examined and analyzed using H&E, Elisa, Immunohistochemistry, Histopathology, 16S rDNA, and Biochemical analysis. To investigate the mechanism of MBD to alleviate AGA using pro-inflammatory factors and intestinal flora.Results: MBD (5.84, 35 g/kg) was administered orally to AGA rats and diclofenac sodium tablets (DS-tablets) were used as standard treatment control. Serum biochemical assessment confirmed that MBD is a safe drug for the treatment of AGA. In addition, our findings confirmed that MBD relieved AGA-related symptoms, such as toe swelling. Lowering serum levels of uric acid, IL-1β, and TGF-β1 immunohistochemical results also confirmed that MBD reduced the expression of inflammatory elements such as IL-1β, NLRP3, ASC, and Caspase-1 in synovial tissue.Furthermore, compared with control group, the 16s rDNA sequencing of AGA rat faeces revealed an increase in the relative abundance of Lachnospiraceae, Muribaculaceae, and Bifidobacteriaceae species. While the relative abundance of Lactobacillaceae, Erysipelotrichaceae, Ruminococcaceae, Prevotellaceae and Enterobacteriaceae showed a relative decrease in species abundance. Of these, the reduction in species abundance of Enterobacteriaceae was associated with a reduction in amino acid metabolism and environmental perception. After MBD therapeutic intervention, the disturbance of the intestinal flora caused by AGA was restored.Conclusion: In summary, MBD is an effective agent for the treatment of AGA, with the potential mechanism being the regulation of intestinal flora to control inflammation. This would help to promote the therapeutic effect of MBD on AGA.https://www.frontiersin.org/articles/10.3389/fphys.2022.1023453/fullBaihu decoctionacute gouty arthritisgut microbiotaproinflammatory cytokinestraditional medicine |
| spellingShingle | Xianyang Wang Haishan Long Ming Chen Zongbo Zhou Qinlin Wu Shijie Xu Geng Li Zhifu Lu Modified Baihu decoction therapeutically remodels gut microbiota to inhibit acute gouty arthritis Frontiers in Physiology Baihu decoction acute gouty arthritis gut microbiota proinflammatory cytokines traditional medicine |
| title | Modified Baihu decoction therapeutically remodels gut microbiota to inhibit acute gouty arthritis |
| title_full | Modified Baihu decoction therapeutically remodels gut microbiota to inhibit acute gouty arthritis |
| title_fullStr | Modified Baihu decoction therapeutically remodels gut microbiota to inhibit acute gouty arthritis |
| title_full_unstemmed | Modified Baihu decoction therapeutically remodels gut microbiota to inhibit acute gouty arthritis |
| title_short | Modified Baihu decoction therapeutically remodels gut microbiota to inhibit acute gouty arthritis |
| title_sort | modified baihu decoction therapeutically remodels gut microbiota to inhibit acute gouty arthritis |
| topic | Baihu decoction acute gouty arthritis gut microbiota proinflammatory cytokines traditional medicine |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2022.1023453/full |
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