Telomere Length in Valve Tissue Is Shorter in Individuals With Aortic Stenosis and in Calcified Valve Areas
BackgroundShort telomere length (TL) is associated with age-related diseases, in particular cardiovascular diseases. However, whether the onset and course of aortic stenosis (AS) is linked to TL in aortic valves remains unknown.ObjectivesTo assess telomere dynamics (TL and telomerase activity) in ao...
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| Format: | Article |
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2021.618335/full |
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| author | Ilona Saraieva Athanase Benetos Athanase Benetos Carlos Labat Anders Franco-Cereceda Anders Franco-Cereceda Magnus Bäck Magnus Bäck Magnus Bäck Magnus Bäck Simon Toupance |
| author_facet | Ilona Saraieva Athanase Benetos Athanase Benetos Carlos Labat Anders Franco-Cereceda Anders Franco-Cereceda Magnus Bäck Magnus Bäck Magnus Bäck Magnus Bäck Simon Toupance |
| author_sort | Ilona Saraieva |
| collection | DOAJ |
| description | BackgroundShort telomere length (TL) is associated with age-related diseases, in particular cardiovascular diseases. However, whether the onset and course of aortic stenosis (AS) is linked to TL in aortic valves remains unknown.ObjectivesTo assess telomere dynamics (TL and telomerase activity) in aortic valves and the possible implication of TL in onset and course of AS.MethodsDNA was extracted from aortic valves obtained from 55 patients (78.2% men; age, 37–79 years), who had undergone replacement surgery due to AS (AS group, n = 32), aortic valve regurgitation and aortic dilation (Non-AS group, n = 23). TL was measured by telomere restriction fragment analysis (TRF) in calcified and non-calcified aortic valve areas. Telomerase activity was evaluated using telomerase repeat amplification protocol (TRAP) in protein extracts from non-calcified and calcified areas of valves obtained from 4 additional patients (50% men; age, 27–70 years).ResultsTL was shorter in calcified aortic valve areas in comparison to non-calcified areas (n = 31, 8.58 ± 0.73 kb vs. 8.12 ± 0.75 kb, p < 0.0001), whereas telomerase activity was not detected in any of those areas. Moreover, patients from AS group displayed shorter telomeres in non-calcified areas than those from the Non-AS group (8.40 ± 0.64 kb vs. 8.85 ± 0.65, p = 0.01).ConclusionsShort telomeres in aortic valves may participate in the development of AS, while concurrently the calcification process seems to promote further local decrease of TL in calcified areas of valves. |
| first_indexed | 2024-12-19T06:12:21Z |
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| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-19T06:12:21Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-4db558d6ddc4430f9ab39450bc8e67c72022-12-21T20:32:58ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-03-01910.3389/fcell.2021.618335618335Telomere Length in Valve Tissue Is Shorter in Individuals With Aortic Stenosis and in Calcified Valve AreasIlona Saraieva0Athanase Benetos1Athanase Benetos2Carlos Labat3Anders Franco-Cereceda4Anders Franco-Cereceda5Magnus Bäck6Magnus Bäck7Magnus Bäck8Magnus Bäck9Simon Toupance10INSERM, DCAC, Université de Lorraine, Nancy, FranceINSERM, DCAC, Université de Lorraine, Nancy, FranceCHRU-Nancy, Pôle “Maladies du Vieillissement, Gérontologie et Soins Palliatifs”, Université de Lorraine, Nancy, FranceINSERM, DCAC, Université de Lorraine, Nancy, FranceKarolinska University Hospital, Theme Heart and Vessels, Stockholm, SwedenDepartment of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, SwedenINSERM, DCAC, Université de Lorraine, Nancy, FranceCHRU-Nancy, Pôle “Maladies du Vieillissement, Gérontologie et Soins Palliatifs”, Université de Lorraine, Nancy, FranceKarolinska University Hospital, Theme Heart and Vessels, Stockholm, SwedenDepartment of Medicine, Karolinska Institutet, Solna, Stockholm, SwedenINSERM, DCAC, Université de Lorraine, Nancy, FranceBackgroundShort telomere length (TL) is associated with age-related diseases, in particular cardiovascular diseases. However, whether the onset and course of aortic stenosis (AS) is linked to TL in aortic valves remains unknown.ObjectivesTo assess telomere dynamics (TL and telomerase activity) in aortic valves and the possible implication of TL in onset and course of AS.MethodsDNA was extracted from aortic valves obtained from 55 patients (78.2% men; age, 37–79 years), who had undergone replacement surgery due to AS (AS group, n = 32), aortic valve regurgitation and aortic dilation (Non-AS group, n = 23). TL was measured by telomere restriction fragment analysis (TRF) in calcified and non-calcified aortic valve areas. Telomerase activity was evaluated using telomerase repeat amplification protocol (TRAP) in protein extracts from non-calcified and calcified areas of valves obtained from 4 additional patients (50% men; age, 27–70 years).ResultsTL was shorter in calcified aortic valve areas in comparison to non-calcified areas (n = 31, 8.58 ± 0.73 kb vs. 8.12 ± 0.75 kb, p < 0.0001), whereas telomerase activity was not detected in any of those areas. Moreover, patients from AS group displayed shorter telomeres in non-calcified areas than those from the Non-AS group (8.40 ± 0.64 kb vs. 8.85 ± 0.65, p = 0.01).ConclusionsShort telomeres in aortic valves may participate in the development of AS, while concurrently the calcification process seems to promote further local decrease of TL in calcified areas of valves.https://www.frontiersin.org/articles/10.3389/fcell.2021.618335/fulltelomere lengthaortic valveaortic stenosiscalcificationaging |
| spellingShingle | Ilona Saraieva Athanase Benetos Athanase Benetos Carlos Labat Anders Franco-Cereceda Anders Franco-Cereceda Magnus Bäck Magnus Bäck Magnus Bäck Magnus Bäck Simon Toupance Telomere Length in Valve Tissue Is Shorter in Individuals With Aortic Stenosis and in Calcified Valve Areas Frontiers in Cell and Developmental Biology telomere length aortic valve aortic stenosis calcification aging |
| title | Telomere Length in Valve Tissue Is Shorter in Individuals With Aortic Stenosis and in Calcified Valve Areas |
| title_full | Telomere Length in Valve Tissue Is Shorter in Individuals With Aortic Stenosis and in Calcified Valve Areas |
| title_fullStr | Telomere Length in Valve Tissue Is Shorter in Individuals With Aortic Stenosis and in Calcified Valve Areas |
| title_full_unstemmed | Telomere Length in Valve Tissue Is Shorter in Individuals With Aortic Stenosis and in Calcified Valve Areas |
| title_short | Telomere Length in Valve Tissue Is Shorter in Individuals With Aortic Stenosis and in Calcified Valve Areas |
| title_sort | telomere length in valve tissue is shorter in individuals with aortic stenosis and in calcified valve areas |
| topic | telomere length aortic valve aortic stenosis calcification aging |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2021.618335/full |
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