Investigation of Interaction between the Spike Protein of SARS-CoV-2 and ACE2-Expressing Cells Using an In Vitro Cell Capturing System
Abstract Background The Interaction between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein with Angiotensin converting enzyme 2 (ACE2) on the host cells is a crucial step for the viral entry and infection. Therefore, investigating the molecular mechanism underlying the in...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-08-01
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| Series: | Biological Procedures Online |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12575-021-00153-9 |
| _version_ | 1819120928678739968 |
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| author | Yuning Shang Feixiang Chen Shasha Li Lijuan Song Yunzhen Gao Xinhua Yu Junfeng Zheng |
| author_facet | Yuning Shang Feixiang Chen Shasha Li Lijuan Song Yunzhen Gao Xinhua Yu Junfeng Zheng |
| author_sort | Yuning Shang |
| collection | DOAJ |
| description | Abstract Background The Interaction between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein with Angiotensin converting enzyme 2 (ACE2) on the host cells is a crucial step for the viral entry and infection. Therefore, investigating the molecular mechanism underlying the interaction is of great importance for the prevention of the infection of SARS-CoV-2. In this study, we aimed to establish a virus-free in vitro system to study the interaction between the spike protein and host cells of SARS-CoV-2. Results Our results show that ACE2-overexpressing HEK293T cells are captured by immobilized spike S1 protein, and the cell capturing process can be inhibited by the receptor binding domain of the spike protein or antibodies against S protein. Furthermore, spike S1 protein variant with D614G mutant show a higher cell capturing ability than wild type spike S1 protein and stronger binding capacity of its receptor ACE2. In addition, the captured cells can be eluted as living cells for further investigation. Conclusions This study provides a new in vitro system for investigating the interaction between SARS-CoV-2 and host cells and purifying ACE2-expressing cells. |
| first_indexed | 2024-12-22T06:28:28Z |
| format | Article |
| id | doaj.art-4db7c1b72dce4363a84ae6302bb2a7ef |
| institution | Directory Open Access Journal |
| issn | 1480-9222 |
| language | English |
| last_indexed | 2024-12-22T06:28:28Z |
| publishDate | 2021-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Procedures Online |
| spelling | doaj.art-4db7c1b72dce4363a84ae6302bb2a7ef2022-12-21T18:35:47ZengBMCBiological Procedures Online1480-92222021-08-012311910.1186/s12575-021-00153-9Investigation of Interaction between the Spike Protein of SARS-CoV-2 and ACE2-Expressing Cells Using an In Vitro Cell Capturing SystemYuning Shang0Feixiang Chen1Shasha Li2Lijuan Song3Yunzhen Gao4Xinhua Yu5Junfeng Zheng6Institute of Psychiatry and Neuroscience, Xinxiang Medical UniversityInstitute of Psychiatry and Neuroscience, Xinxiang Medical UniversityInstitute of Psychiatry and Neuroscience, Xinxiang Medical UniversityInstitute of Psychiatry and Neuroscience, Xinxiang Medical UniversityInstitute of Psychiatry and Neuroscience, Xinxiang Medical UniversityPriority Area Asthma & Allergy, Research Center Borstel, Airway Research Center North (ARCN), Members of the German Center for Lung Research (DZL)Institute of Psychiatry and Neuroscience, Xinxiang Medical UniversityAbstract Background The Interaction between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein with Angiotensin converting enzyme 2 (ACE2) on the host cells is a crucial step for the viral entry and infection. Therefore, investigating the molecular mechanism underlying the interaction is of great importance for the prevention of the infection of SARS-CoV-2. In this study, we aimed to establish a virus-free in vitro system to study the interaction between the spike protein and host cells of SARS-CoV-2. Results Our results show that ACE2-overexpressing HEK293T cells are captured by immobilized spike S1 protein, and the cell capturing process can be inhibited by the receptor binding domain of the spike protein or antibodies against S protein. Furthermore, spike S1 protein variant with D614G mutant show a higher cell capturing ability than wild type spike S1 protein and stronger binding capacity of its receptor ACE2. In addition, the captured cells can be eluted as living cells for further investigation. Conclusions This study provides a new in vitro system for investigating the interaction between SARS-CoV-2 and host cells and purifying ACE2-expressing cells.https://doi.org/10.1186/s12575-021-00153-9SARS-CoV-2;spike proteinACE2In vitro cell-capture |
| spellingShingle | Yuning Shang Feixiang Chen Shasha Li Lijuan Song Yunzhen Gao Xinhua Yu Junfeng Zheng Investigation of Interaction between the Spike Protein of SARS-CoV-2 and ACE2-Expressing Cells Using an In Vitro Cell Capturing System Biological Procedures Online SARS-CoV-2;spike protein ACE2 In vitro cell-capture |
| title | Investigation of Interaction between the Spike Protein of SARS-CoV-2 and ACE2-Expressing Cells Using an In Vitro Cell Capturing System |
| title_full | Investigation of Interaction between the Spike Protein of SARS-CoV-2 and ACE2-Expressing Cells Using an In Vitro Cell Capturing System |
| title_fullStr | Investigation of Interaction between the Spike Protein of SARS-CoV-2 and ACE2-Expressing Cells Using an In Vitro Cell Capturing System |
| title_full_unstemmed | Investigation of Interaction between the Spike Protein of SARS-CoV-2 and ACE2-Expressing Cells Using an In Vitro Cell Capturing System |
| title_short | Investigation of Interaction between the Spike Protein of SARS-CoV-2 and ACE2-Expressing Cells Using an In Vitro Cell Capturing System |
| title_sort | investigation of interaction between the spike protein of sars cov 2 and ace2 expressing cells using an in vitro cell capturing system |
| topic | SARS-CoV-2;spike protein ACE2 In vitro cell-capture |
| url | https://doi.org/10.1186/s12575-021-00153-9 |
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