Anti-Inflammatory and Antiplatelet Activities of Plasma Are Conserved Across Twelve Mammalian Species
Human plasma inhibits arachidonic acid metabolism and platelet aggregation. This helps human form a haemostatic control system that prevents the progress of certain aggregatory or inflammatory reactions. Whether this property of plasma is unique to human or extends to other species is not well known...
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MDPI AG
2014-07-01
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| 丛编: | Molecules |
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| 在线阅读: | http://www.mdpi.com/1420-3049/19/8/11385 |
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| author | Sagheer Ahmed Saima Gul Fazean Idris Abrar Hussain Muhammad Zia-Ul-Haq Hawa Z. E. Jaafar Marius Moga |
| author_facet | Sagheer Ahmed Saima Gul Fazean Idris Abrar Hussain Muhammad Zia-Ul-Haq Hawa Z. E. Jaafar Marius Moga |
| author_sort | Sagheer Ahmed |
| collection | DOAJ |
| description | Human plasma inhibits arachidonic acid metabolism and platelet aggregation. This helps human form a haemostatic control system that prevents the progress of certain aggregatory or inflammatory reactions. Whether this property of plasma is unique to human or extends to other species is not well known. It is speculated that this protective ability of plasma remains evolutionarily conserved in different mammals. In order to confirm this, the effect of plasma from 12 different mammalian species was investigated for its inhibitory potential against arachidonic acid metabolism and platelet aggregation. Metabolism of arachidonic acid by cyclooxygenase and lipoxygenase pathways was studies using radio-immuno assay and thin layer chromatography while platelet aggregation in the plasma of various mammals was monitored following turbedmetric method in a dual channel aggregometer. Results indicate that inhibition of AA metabolism and platelet aggregation is a common feature of plasma obtained from different mammalian species, although there exists large interspecies variation. This shows that besides human, other mammals also possess general protective mechanisms against various aggregatory and inflammatory conditions and this anti-inflammatory property of the plasma is evolutionarily conserved in mammalian species. The most likely candidates responsible for these properties of plasma include haptoglobin, albumin and lipoproteins. |
| first_indexed | 2024-12-10T07:08:27Z |
| format | Article |
| id | doaj.art-4dba14b51daa4bfd9b6895a05ca2a7ed |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T07:08:27Z |
| publishDate | 2014-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-4dba14b51daa4bfd9b6895a05ca2a7ed2022-12-22T01:58:07ZengMDPI AGMolecules1420-30492014-07-01198113851139410.3390/molecules190811385molecules190811385Anti-Inflammatory and Antiplatelet Activities of Plasma Are Conserved Across Twelve Mammalian SpeciesSagheer Ahmed0Saima Gul1Fazean Idris2Abrar Hussain3Muhammad Zia-Ul-Haq4Hawa Z. E. Jaafar5Marius Moga6PAP RSB Institute of Health Sciences, University Brunei Darussalam, Gadong BE1410, Bander Seri Begawan, Brunei DarussalamPAP RSB Institute of Health Sciences, University Brunei Darussalam, Gadong BE1410, Bander Seri Begawan, Brunei DarussalamPAP RSB Institute of Health Sciences, University Brunei Darussalam, Gadong BE1410, Bander Seri Begawan, Brunei DarussalamDepartment of Biotechnology & Informtics, Baluchistan University of Information Technology, Engineering & Management Sciences, Quetta 87300, PakistanThe Patent Office, Karachi 74400, PakistanDepartment of Crop Science, Faculty of Agriculture, University Putra Malaysia, Selangor 43400, MalaysiaDepartment of Medical and Surgical Specialities, Transilvania University of Brasov, Brasov 500036, RomaniaHuman plasma inhibits arachidonic acid metabolism and platelet aggregation. This helps human form a haemostatic control system that prevents the progress of certain aggregatory or inflammatory reactions. Whether this property of plasma is unique to human or extends to other species is not well known. It is speculated that this protective ability of plasma remains evolutionarily conserved in different mammals. In order to confirm this, the effect of plasma from 12 different mammalian species was investigated for its inhibitory potential against arachidonic acid metabolism and platelet aggregation. Metabolism of arachidonic acid by cyclooxygenase and lipoxygenase pathways was studies using radio-immuno assay and thin layer chromatography while platelet aggregation in the plasma of various mammals was monitored following turbedmetric method in a dual channel aggregometer. Results indicate that inhibition of AA metabolism and platelet aggregation is a common feature of plasma obtained from different mammalian species, although there exists large interspecies variation. This shows that besides human, other mammals also possess general protective mechanisms against various aggregatory and inflammatory conditions and this anti-inflammatory property of the plasma is evolutionarily conserved in mammalian species. The most likely candidates responsible for these properties of plasma include haptoglobin, albumin and lipoproteins.http://www.mdpi.com/1420-3049/19/8/11385platelet aggregationarachidonic acidanti-inflammatorymammalsevolution |
| spellingShingle | Sagheer Ahmed Saima Gul Fazean Idris Abrar Hussain Muhammad Zia-Ul-Haq Hawa Z. E. Jaafar Marius Moga Anti-Inflammatory and Antiplatelet Activities of Plasma Are Conserved Across Twelve Mammalian Species Molecules platelet aggregation arachidonic acid anti-inflammatory mammals evolution |
| title | Anti-Inflammatory and Antiplatelet Activities of Plasma Are Conserved Across Twelve Mammalian Species |
| title_full | Anti-Inflammatory and Antiplatelet Activities of Plasma Are Conserved Across Twelve Mammalian Species |
| title_fullStr | Anti-Inflammatory and Antiplatelet Activities of Plasma Are Conserved Across Twelve Mammalian Species |
| title_full_unstemmed | Anti-Inflammatory and Antiplatelet Activities of Plasma Are Conserved Across Twelve Mammalian Species |
| title_short | Anti-Inflammatory and Antiplatelet Activities of Plasma Are Conserved Across Twelve Mammalian Species |
| title_sort | anti inflammatory and antiplatelet activities of plasma are conserved across twelve mammalian species |
| topic | platelet aggregation arachidonic acid anti-inflammatory mammals evolution |
| url | http://www.mdpi.com/1420-3049/19/8/11385 |
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