Viscosupplementation may preserve tibial cartilage and collagen in osteoarthritis: findings from a preclinical model of osteoarthritis
Abstract Purpose Intraarticular (IA) hyaluronic acid (HA) injection is used to reduce pain and improve mobility in knee osteoarthritis (OA). Little is known about histopathological changes underlying HA efficacy. This study investigated dose-related effects of 1% sodium hyaluronate (BioHA) on knee j...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-05-01
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| Series: | Journal of Experimental Orthopaedics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40634-020-00256-4 |
| _version_ | 1797333221469323264 |
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| author | John Lokhnauth Kevin E. Driscoll Alison Bendele Faizan Niazi Alfred Liang Crilles C. Larsen |
| author_facet | John Lokhnauth Kevin E. Driscoll Alison Bendele Faizan Niazi Alfred Liang Crilles C. Larsen |
| author_sort | John Lokhnauth |
| collection | DOAJ |
| description | Abstract Purpose Intraarticular (IA) hyaluronic acid (HA) injection is used to reduce pain and improve mobility in knee osteoarthritis (OA). Little is known about histopathological changes underlying HA efficacy. This study investigated dose-related effects of 1% sodium hyaluronate (BioHA) on knee joint histopathology and pain responses in a medial meniscal tear (MMT) rat model of OA. Methods Following MMT surgery, rats were randomized into treatment groups: single IA injection of vehicle, BioHA, or an avian-derived hyaluronic acid (hylan G-F 20) on Day 7; or 3 weekly injections of vehicle or BioHA on Days 7, 14, and 21. On Day 35, joints were evaluated by microscopic histopathology for cartilage degeneration, collagen degeneration, synovitis, and cytokine expression (tumor necrosis factor α, transforming growth factor β). Results Joint pathology for control animals was consistent with that expected for the MMT model. Rats treated with 3 injections of IA-BioHA had significantly reduced collagen degeneration (21%) relative to control animals. No significant change in collagen degeneration was observed for rats given a single injection of hylan G-F 20 or IA-BioHA compared to control animals. HA treatment did not affect cytokine expression. Conclusions IA-BioHA viscosupplementation in a rat MMT model of OA showed preservation of joint cartilage and collagen. This effect was most pronounced on tibial surfaces having less severe injury, suggesting that treatment should be initiated early in the disease process. A comparison of responses to IA-BioHA or hylan G-F 20 in the MMT rat OA model suggest IA-BioHA may be more effective in preserving joint connective tissue. |
| first_indexed | 2024-03-08T08:00:32Z |
| format | Article |
| id | doaj.art-4dbf1cf1d9314118b7ed3ebb072c477f |
| institution | Directory Open Access Journal |
| issn | 2197-1153 |
| language | English |
| last_indexed | 2024-03-08T08:00:32Z |
| publishDate | 2020-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Experimental Orthopaedics |
| spelling | doaj.art-4dbf1cf1d9314118b7ed3ebb072c477f2024-02-02T12:24:49ZengSpringerOpenJournal of Experimental Orthopaedics2197-11532020-05-017111110.1186/s40634-020-00256-4Viscosupplementation may preserve tibial cartilage and collagen in osteoarthritis: findings from a preclinical model of osteoarthritisJohn Lokhnauth0Kevin E. Driscoll1Alison Bendele2Faizan Niazi3Alfred Liang4Crilles C. Larsen5Ferring Pharmaceuticals Inc.Healthcare Innovation PartnersBolder BioPATHFerring Pharmaceuticals Inc.Ferring Pharmaceuticals Inc.Ferring Pharmaceuticals Inc.Abstract Purpose Intraarticular (IA) hyaluronic acid (HA) injection is used to reduce pain and improve mobility in knee osteoarthritis (OA). Little is known about histopathological changes underlying HA efficacy. This study investigated dose-related effects of 1% sodium hyaluronate (BioHA) on knee joint histopathology and pain responses in a medial meniscal tear (MMT) rat model of OA. Methods Following MMT surgery, rats were randomized into treatment groups: single IA injection of vehicle, BioHA, or an avian-derived hyaluronic acid (hylan G-F 20) on Day 7; or 3 weekly injections of vehicle or BioHA on Days 7, 14, and 21. On Day 35, joints were evaluated by microscopic histopathology for cartilage degeneration, collagen degeneration, synovitis, and cytokine expression (tumor necrosis factor α, transforming growth factor β). Results Joint pathology for control animals was consistent with that expected for the MMT model. Rats treated with 3 injections of IA-BioHA had significantly reduced collagen degeneration (21%) relative to control animals. No significant change in collagen degeneration was observed for rats given a single injection of hylan G-F 20 or IA-BioHA compared to control animals. HA treatment did not affect cytokine expression. Conclusions IA-BioHA viscosupplementation in a rat MMT model of OA showed preservation of joint cartilage and collagen. This effect was most pronounced on tibial surfaces having less severe injury, suggesting that treatment should be initiated early in the disease process. A comparison of responses to IA-BioHA or hylan G-F 20 in the MMT rat OA model suggest IA-BioHA may be more effective in preserving joint connective tissue.http://link.springer.com/article/10.1186/s40634-020-00256-4OsteoarthritisHyaluronic acidCollagenViscosupplementation |
| spellingShingle | John Lokhnauth Kevin E. Driscoll Alison Bendele Faizan Niazi Alfred Liang Crilles C. Larsen Viscosupplementation may preserve tibial cartilage and collagen in osteoarthritis: findings from a preclinical model of osteoarthritis Journal of Experimental Orthopaedics Osteoarthritis Hyaluronic acid Collagen Viscosupplementation |
| title | Viscosupplementation may preserve tibial cartilage and collagen in osteoarthritis: findings from a preclinical model of osteoarthritis |
| title_full | Viscosupplementation may preserve tibial cartilage and collagen in osteoarthritis: findings from a preclinical model of osteoarthritis |
| title_fullStr | Viscosupplementation may preserve tibial cartilage and collagen in osteoarthritis: findings from a preclinical model of osteoarthritis |
| title_full_unstemmed | Viscosupplementation may preserve tibial cartilage and collagen in osteoarthritis: findings from a preclinical model of osteoarthritis |
| title_short | Viscosupplementation may preserve tibial cartilage and collagen in osteoarthritis: findings from a preclinical model of osteoarthritis |
| title_sort | viscosupplementation may preserve tibial cartilage and collagen in osteoarthritis findings from a preclinical model of osteoarthritis |
| topic | Osteoarthritis Hyaluronic acid Collagen Viscosupplementation |
| url | http://link.springer.com/article/10.1186/s40634-020-00256-4 |
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