Relationship between poultry biosecurity assessments and Escherichia coli prevalence in poultry farms
A crucial first step in preventing the introduction and spread of harmful microorganisms in poultry farms is biosecurity. The objectives of this investigation were to assess biosecurity measures and the prevalence of different E. coli species in commercial chicken farms in Sharkia province, Egypt....
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Assiut University
2024-03-01
|
| Series: | Journal of Advanced Veterinary Research |
| Subjects: | |
| Online Access: | https://www.advetresearch.com/index.php/AVR/article/view/1561 |
| _version_ | 1797267244062867456 |
|---|---|
| author | Mahmoud S. Zaki Hanan A. Fahmy Mariam H.A. Khedr Mai A.A. Goha Amira S.A. Attia |
| author_facet | Mahmoud S. Zaki Hanan A. Fahmy Mariam H.A. Khedr Mai A.A. Goha Amira S.A. Attia |
| author_sort | Mahmoud S. Zaki |
| collection | DOAJ |
| description |
A crucial first step in preventing the introduction and spread of harmful microorganisms in poultry farms is biosecurity. The objectives of this investigation were to assess biosecurity measures and the prevalence of different E. coli species in commercial chicken farms in Sharkia province, Egypt. Sensitivity of E. coli strains was identified, and their susceptibility to antibiotics and disinfection was evaluated as well. Four farms provided 21 samples each, for a total of 84 samples. Three samples of each were collected for water, feed, litter, cloaca, wall, hand, and foot boots. All the studied farms obtained a "poor biosecurity" grade, meaning that their overall biosecurity score, which is comprised of 40% external and 36.54% internal biosecurity, was less than 50%. Escherichia coli species were found in 79.76% of the samples. It was isolated from water, feed, litter, cloaca, walls, hands, and foot boots with percentages of 91.6; 83.3; 91.6; 100; 58.3; 58.3 and 75%, respectively. Biosecurity level had significant negative correlations with the isolated E. coil species. Escherichia coli O119 was the most common serotype in litter and the cloaca, while E. coli O1 was the most prevalent serotype in walls and hands. Additionally, serotypes for E. coli O26, O159, O128 and O78 were included. According to the patterns of antibiotic sensitivity, amikacin, nitrofurantoin, gentamycin, and levofloxacin showed intermediate sensitivity, whereas E. coli O119 bacteria were highly sensitive to ciprofloxacin. In the absence and presence of organic matter, Virocid, Cid 2000 and finally Virkon S were the most efficient against E. coli O119. According to this study, there were differences in the investigated farms' biosecurity levels in relation to the occurrence of Escherichia coli. To reduce the introduction and persistence of E. coli in poultry farms, several biosecurity practices and management, including stringent cleaning and disinfection measures should be applied.
|
| first_indexed | 2024-03-07T14:01:59Z |
| format | Article |
| id | doaj.art-4dc1807627974d34bb7da07438710d83 |
| institution | Directory Open Access Journal |
| issn | 2090-6269 2090-6277 |
| language | English |
| last_indexed | 2024-04-25T01:13:30Z |
| publishDate | 2024-03-01 |
| publisher | Assiut University |
| record_format | Article |
| series | Journal of Advanced Veterinary Research |
| spelling | doaj.art-4dc1807627974d34bb7da07438710d832024-03-10T05:05:13ZengAssiut UniversityJournal of Advanced Veterinary Research2090-62692090-62772024-03-01143Relationship between poultry biosecurity assessments and Escherichia coli prevalence in poultry farmsMahmoud S. Zaki0Hanan A. Fahmy1Mariam H.A. Khedr2Mai A.A. Goha3Amira S.A. Attia4Department of Veterinary Public Health, Faculty of Veterinary Medicine, Zagazig University, Egypt.Researcher and Environmental Affairs, Animal Health Research Institute (AHRI), Agriculture Research Centre (ARC), Dokki, Giza, Egypt.Department of Veterinary Public Health, Faculty of Veterinary Medicine, Zagazig University, Egypt.Department of Veterinary Public Health, Faculty of Veterinary Medicine, Zagazig University, Egypt.Department of Veterinary Public Health, Faculty of Veterinary Medicine, Zagazig University, Egypt. A crucial first step in preventing the introduction and spread of harmful microorganisms in poultry farms is biosecurity. The objectives of this investigation were to assess biosecurity measures and the prevalence of different E. coli species in commercial chicken farms in Sharkia province, Egypt. Sensitivity of E. coli strains was identified, and their susceptibility to antibiotics and disinfection was evaluated as well. Four farms provided 21 samples each, for a total of 84 samples. Three samples of each were collected for water, feed, litter, cloaca, wall, hand, and foot boots. All the studied farms obtained a "poor biosecurity" grade, meaning that their overall biosecurity score, which is comprised of 40% external and 36.54% internal biosecurity, was less than 50%. Escherichia coli species were found in 79.76% of the samples. It was isolated from water, feed, litter, cloaca, walls, hands, and foot boots with percentages of 91.6; 83.3; 91.6; 100; 58.3; 58.3 and 75%, respectively. Biosecurity level had significant negative correlations with the isolated E. coil species. Escherichia coli O119 was the most common serotype in litter and the cloaca, while E. coli O1 was the most prevalent serotype in walls and hands. Additionally, serotypes for E. coli O26, O159, O128 and O78 were included. According to the patterns of antibiotic sensitivity, amikacin, nitrofurantoin, gentamycin, and levofloxacin showed intermediate sensitivity, whereas E. coli O119 bacteria were highly sensitive to ciprofloxacin. In the absence and presence of organic matter, Virocid, Cid 2000 and finally Virkon S were the most efficient against E. coli O119. According to this study, there were differences in the investigated farms' biosecurity levels in relation to the occurrence of Escherichia coli. To reduce the introduction and persistence of E. coli in poultry farms, several biosecurity practices and management, including stringent cleaning and disinfection measures should be applied. https://www.advetresearch.com/index.php/AVR/article/view/1561Escherichia coli Biosecurity Antibiotics Disinfectants |
| spellingShingle | Mahmoud S. Zaki Hanan A. Fahmy Mariam H.A. Khedr Mai A.A. Goha Amira S.A. Attia Relationship between poultry biosecurity assessments and Escherichia coli prevalence in poultry farms Journal of Advanced Veterinary Research Escherichia coli Biosecurity Antibiotics Disinfectants |
| title | Relationship between poultry biosecurity assessments and Escherichia coli prevalence in poultry farms |
| title_full | Relationship between poultry biosecurity assessments and Escherichia coli prevalence in poultry farms |
| title_fullStr | Relationship between poultry biosecurity assessments and Escherichia coli prevalence in poultry farms |
| title_full_unstemmed | Relationship between poultry biosecurity assessments and Escherichia coli prevalence in poultry farms |
| title_short | Relationship between poultry biosecurity assessments and Escherichia coli prevalence in poultry farms |
| title_sort | relationship between poultry biosecurity assessments and escherichia coli prevalence in poultry farms |
| topic | Escherichia coli Biosecurity Antibiotics Disinfectants |
| url | https://www.advetresearch.com/index.php/AVR/article/view/1561 |
| work_keys_str_mv | AT mahmoudszaki relationshipbetweenpoultrybiosecurityassessmentsandescherichiacoliprevalenceinpoultryfarms AT hananafahmy relationshipbetweenpoultrybiosecurityassessmentsandescherichiacoliprevalenceinpoultryfarms AT mariamhakhedr relationshipbetweenpoultrybiosecurityassessmentsandescherichiacoliprevalenceinpoultryfarms AT maiaagoha relationshipbetweenpoultrybiosecurityassessmentsandescherichiacoliprevalenceinpoultryfarms AT amirasaattia relationshipbetweenpoultrybiosecurityassessmentsandescherichiacoliprevalenceinpoultryfarms |