The Effect of the <i>BCO2</i> Genotype on the Expression of Genes Related to Carotenoid, Retinol, and α-Tocopherol Metabolism in Rabbits Fed a Diet with Aztec Marigold Flower Extract

The Effect of the <i>BCO2</i> Genotype on the Expression of Genes Related to Carotenoid, Retinol, and α-Tocopherol Metabolism in Rabbits Fed a Diet with Aztec Marigold Flower Extract

This study investigated the effect of the <i>BCO2</i> genotype and the addition of Aztec marigold flower extract to rabbit diets on the expression of <i>BCO1</i>, <i>BCO2</i>, <i>LRAT</i>, and <i>TTPA</i> genes in the liver. The levels of l...

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Bibliographic Details
Main Authors: Janusz Strychalski, Andrzej Gugołek, Zofia Antoszkiewicz, Dorota Fopp-Bayat, Edyta Kaczorek-Łukowska, Anna Snarska, Grzegorz Zwierzchowski, Angelika Król-Grzymała, Paulius Matusevičius
Format: Article
Language:English
Published: MDPI AG 2022-09-01
Series:International Journal of Molecular Sciences
Subjects:
<i>BCO2</i> gene
rabbits
marigold
gene expression
carotenoids
Online Access:https://www.mdpi.com/1422-0067/23/18/10552
Description
Summary:This study investigated the effect of the <i>BCO2</i> genotype and the addition of Aztec marigold flower extract to rabbit diets on the expression of <i>BCO1</i>, <i>BCO2</i>, <i>LRAT</i>, and <i>TTPA</i> genes in the liver. The levels of lutein, zeaxanthin, β-carotene, retinol, and α-tocopherol in the liver and blood serum of rabbits, as well as plasma biochemical parameters and serum antioxidant enzyme activities were also determined. Sixty male Termond White growing rabbits were divided into three groups based on their genotype at codon 248 of the <i>BCO2</i> gene (ins/ins, ins/del and del/del); each group was divided into two subgroups: one subgroup received a standard diet, and the other subgroup was fed a diet supplemented with 6 g/kg of marigold flower extract. The obtained results indicate that the <i>BCO2</i> genotype may affect the expression levels of <i>BCO1</i> and <i>BCO2</i> genes in rabbits. Moreover, the addition of marigold extract to the diet of <i>BCO2</i> del/del rabbits may increase the expression level of the <i>BCO2</i> gene. Finally, an increase in the amount of lutein in the diet of rabbits with the <i>BCO2</i> del/del genotype contributes to its increased accumulation in the liver and blood of animals without compromising their health status or liver function.
ISSN:1661-6596
1422-0067

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